Elevated expression levels of COX-2, IL-8 and VEGF in colon adenocarcinoma

Simple item page
dc.contributor.authorUslukaya, Omer
dc.contributor.authorYegin, Zeynep
dc.contributor.authorTaskesen, Fatih
dc.contributor.authorYildirim, Ibrahim Halil
dc.date.accessioned2025-03-23T19:30:23Z
dc.date.available2025-03-23T19:30:23Z
dc.date.issued2023
dc.departmentSinop Üniversitesi
dc.description.abstractThere is growing evidence of a connection between inflammation and tumor development and NF-kappa B is an important transcription factor in the inflammation pathway. Genetic approaches have proven the role of NF-kappa B responsive genes in tumorigenesis. The NF-kappa B responsive genes products such as IL-8, VEGF and COX-2 are the key components of angiogenesis. MMP-2 and MMP-9 are playing important roles in the disruption of the extracellular matrix that may contribute to the metastasis of tumor cells. This study aimed to investigate gene expression levels of COX-2, IL-8, VEGF, MMP-2 and MMP-9 in colon tumors. A total of 34 fresh colon carcinoma specimens and paired normal adjacent tissues (NAT) were collected during the surgery and RNA isolations were carried out from specimens. Synthesis of cDNA was carried out from these RNAs with oligo dT18 primers. The transcribed cDNA was used for PCR amplification reactions for the investigated genes with beta-actin being the internal reference via the semi-quantitative RT-PCR method. A statistically significant difference was observed for COX-2, IL-8 and VEGF which were all upregulated in colon tumors compared with adjacent normal tissues (p<0.05). However, MMP-2 and MMP-9 expression levels did not change between tumor and normal tissues (p>0.05). Upregulated expression levels of COX-2, IL-8 and VEGF might occur in the early stages of tumorigenesis and detection of these mRNA levels may be beneficial for early diagnosis and management of colon tumors.
dc.description.sponsorshipDicle University Scientific Research Projects Coordination Unit; [11-VF-76]
dc.description.sponsorshipThis work was supported by Dicle University Scientific Research Projects Coordination Unit. Project Number: 11-VF-76.
dc.identifier.doi10.14715/cmb/2023.69.6.22
dc.identifier.endpage150
dc.identifier.issn0145-5680
dc.identifier.issn1165-158X
dc.identifier.issue6
dc.identifier.pmid37605577
dc.identifier.scopus2-s2.0-85168498482
dc.identifier.scopusqualityQ4
dc.identifier.startpage146
dc.identifier.urihttps://doi.org/10.14715/cmb/2023.69.6.22
dc.identifier.urihttps://hdl.handle.net/11486/5084
dc.identifier.volume69
dc.identifier.wosWOS:001059104800022
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherC M B Assoc
dc.relation.ispartofCellular and Molecular Biology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_WOS_20250323
dc.subjectColon cancer
dc.subjectCOX-2
dc.subjectIL-8
dc.subjectVEGF
dc.subjectMMP-2
dc.subjectMMP-9
dc.titleElevated expression levels of COX-2, IL-8 and VEGF in colon adenocarcinoma
dc.typeArticle

Files

Collections

WoS İndeksli Yayınlar Koleksiyonu
PubMed İndeksli Yayınlar Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu

We collect and process your personal information for the following purposes: Authentication, Preferences, Acknowledgement and Statistics.
To learn more, please read our privacy policy.

Customize