Investigation of physiological, metabolomics analysis and HvSOS1 genes of priming with azelaic acid in barley seeds under salt stress

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dc.contributor.authorCetinkaya, Hatice
dc.contributor.authorStaykov, Nikola
dc.contributor.authorGechev, Tsanko
dc.contributor.authorDinler, Burcu Seckin
dc.date.accessioned2026-04-25T14:20:00Z
dc.date.available2026-04-25T14:20:00Z
dc.date.issued2025
dc.departmentSinop Üniversitesi
dc.description.abstractAzelaic acid (AzA) is a nine-carbon dicarboxylic acid that plays a role in the systemic acquired resistance (SAR) mechanism. Upon consulting the published data on the topic, many studies were available, dedicated on the mechanism of action of azelaic acid in plants under normal conditions and biotic stress, but remarkably few on the abiotic stress. Barley seeds (Hordeum vulgare L.) from the cultivars B & uuml;lb & uuml;l89 and Avc & imath;2002 (salt tolerant and sensitive), were pre-treated with three different concentrations of azelaic acid (2, 4, 8 ppm) and later the seedlings were exposed to salt stress with 300 mM NaCl. Physiological, biochemical and molecular biological effects of AzA-treatment were examined in leaf samples. Namely, growth, relative water content (RWC), relative electrolyte leakege (REL), leaf area (LA), proline amount, lipid peroxidation product malondialdehyde (MDA), reactive oxygen species and endogenic abscisic acid (ABA) and salicylic acid (SA) levels, as well as metabolomics and SOS1 gene expression levels were determined. According to the obtained results, AzA application on seeds, reduced the oxidative damage caused by salt stress in barley plants and induced improvement in stress parameters (MDA, H2O2, O2 center dot- content, proline and OH center dot- scavenging capacity). In addition, growth parameters (length and weight), chlorophyll content, RWC, LA, SA and GABA levels were suppressed, while proline, REL and metabolomics (methionine, alanine, serine, sucrose, raffinose and mannitol) increased under salt stress. Notably, after AzA application, these parameters showed significant rescue effect at a level approaching the control groups. However, AzA treatment under salt stress increased endogenous ABA levels in both cultivars, especially at higher concentrations (B & uuml;lb & uuml;l89 - 4 ppm, Avc & imath;2002 - 8 ppm). Based on these results, it was suggested that AzA can be used as an oxidative damage inhibitor or signaling molecule for increasing salt tolerance in plants. Overall, this study presents physiological, biochemical, metabolomic and gene expression data which shows that AzA protects against oxidative stress in both barley cultivars, with stronger effects in Avc & imath;2002.
dc.description.sponsorshipEuropean Regional Development Fund through the Bulgarian Program for Research, Innovation, and Digitalization for Smart Transformation (PRIDST) Operational Programme
dc.description.sponsorshipThis work was supported by the Sinop University Research Foundation, Turkiye (FEF-2023-SU102FEF). T.G. and N.S. acknowledge the project BIOCROPS, Grant No. & Kcy;& Pcy;-06-& Dcy;& Bcy;/1, funded by the Bulgarian National Science Fund and the BG16RFPR002-1.014-0003-C01 project, financed by the European Regional Development Fund through the Bulgarian Program for Research, Innovation, and Digitalization for Smart Transformation (PRIDST) Operational Programme.
dc.identifier.doi10.1016/j.plaphy.2025.110728
dc.identifier.issn0981-9428
dc.identifier.issn1873-2690
dc.identifier.orcid0000-0002-3328-0243
dc.identifier.orcid0000-0001-6289-380X
dc.identifier.pmid41202559
dc.identifier.scopus2-s2.0-105023903491
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.1016/j.plaphy.2025.110728
dc.identifier.urihttps://hdl.handle.net/11486/8320
dc.identifier.volume229
dc.identifier.wosWOS:001619334500006
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherElsevier France-Editions Scientifiques Medicales Elsevier
dc.relation.ispartofPlant Physiology and Biochemistry
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WOS_20260420
dc.subjectDicarboxylic acid
dc.subjectGene expression
dc.subjectHormone
dc.subjectMetabolomics
dc.subjectSalinity
dc.titleInvestigation of physiological, metabolomics analysis and HvSOS1 genes of priming with azelaic acid in barley seeds under salt stress
dc.typeArticle

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