Regulation of Nrf2 and Nrf2-related proteins by ganoderma lucidum in hepatocellular carcinoma

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dc.authoridAhmed, Omar Saad/0000-0002-3527-9238
dc.contributor.authorAslaminabad, Ramin
dc.contributor.authorRahimianshahreza, Negin
dc.contributor.authorHosseini, Seyed Amirhossein
dc.contributor.authorArmagan, Guliz
dc.contributor.authorKhan, Ahmad Kashif
dc.contributor.authorOzbolat, Guluzar
dc.contributor.authorAhmed, Omar Saad
dc.date.accessioned2025-03-23T19:44:08Z
dc.date.available2025-03-23T19:44:08Z
dc.date.issued2022
dc.departmentSinop Üniversitesi
dc.description.abstractBackground HCC is among the most common cancer. Ganoderma lucidum (G.lucidum) has been essential in preventing and treating cancer. The Nrf2 signaling cascade is a cell protective mechanism against further damage, such as cancer development. This signaling pathway upregulates the cytoprotective genes and is vital in eliminating xenobiotics and reactive oxygen. This study aimed to show the potential cytotoxic activity of G. lucidum aqueous extract in HCC. Methods and results MTT assay was used to detect cell viability. Nrf2-related proteins were measured by western blotting, and the flow cytometry method assayed cell population in different cycle phases. Cell viability was 49% and 47% following G. lucidum extract at 100 mu g/ml at 24 and 48 h treatments, respectively. G. lucidum extract (aqueous, 100 or 50 mu g/ml) treatments for 24, 48, or 72 h were able to significantly change the cytoplasmic/nuclear amount of Nrf2 and HO-1, NQO1 protein levels. Moreover, at both concentrations, arrest of the G0/G1 cell cycle was stimulated in HCC. Conclusions The activation of the Nrf2 signaling pathways seems to be among the mechanisms underlining the protective and therapeutic action of G. lucidum against HCC.
dc.description.sponsorshipEge Universitesi
dc.description.sponsorshipThis work was supported financially by Ege Universitesi.
dc.identifier.doi10.1007/s11033-022-07862-8
dc.identifier.endpage9612
dc.identifier.issn0301-4851
dc.identifier.issn1573-4978
dc.identifier.issue10
dc.identifier.pmid36038810
dc.identifier.scopus2-s2.0-85136943959
dc.identifier.scopusqualityQ2
dc.identifier.startpage9605
dc.identifier.urihttps://doi.org/10.1007/s11033-022-07862-8
dc.identifier.urihttps://hdl.handle.net/11486/6880
dc.identifier.volume49
dc.identifier.wosWOS:000847259800001
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherSpringer
dc.relation.ispartofMolecular Biology Reports
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WOS_20250323
dc.subjectHepatocellular carcinoma
dc.subjectMedicinal mushroom
dc.subjectNrf-2
dc.subjectCell cycle arrest
dc.titleRegulation of Nrf2 and Nrf2-related proteins by ganoderma lucidum in hepatocellular carcinoma
dc.typeArticle

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