Structural analysis and biological functionalities of iron(III)- and manganese(III)-thiosemicarbazone complexes: in vitro anti-proliferative activity on human cancer cells, DNA binding and cleavage studies

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dc.authorid/0000-0002-3224-1920
dc.authoridKaya, Busra/0000-0003-2706-172X
dc.contributor.authorKaya, Busra
dc.contributor.authorYilmaz, Zehra Kubra
dc.contributor.authorSahin, Onur
dc.contributor.authorAslim, Belma
dc.contributor.authorTukenmez, Ummugulsum
dc.contributor.authorUlkuseven, Bahri
dc.date.accessioned2025-03-23T19:44:33Z
dc.date.available2025-03-23T19:44:33Z
dc.date.issued2019
dc.departmentSinop Üniversitesi
dc.description.abstractOne iron(III) and two manganese(III) complexes based on thiosemicarbazone were synthesized and characterized using analytical and spectroscopic data. The crystallographic analysis showed the square pyramid structures of the complexes. Electronic spectra analysis was performed to determine the nature of the interaction between the complexes and calf thymus DNA (CT-DNA). DNA cleavage activities of the complexes were examined by gel electrophoresis (pBR322 DNA). The cytotoxicity of the complexes was determined against human cervical carcinoma (HeLa) and human colorectal adenocarcinoma (HT-29) cell lines by MTT assay. The results indicated that complex Fe1 is bound to CT-DNA via the intercalation mode, while complexes Mn1 and Mn2 are bound to CT-DNA via groove binding and/or electrostatic interactions rather than the intercalation mode. In addition, they showed good binding activity, which followed the order of Fe1>Mn2>Mn1. Complexes were found to promote the cleavage of DNA from supercoiled form (SC, Form I) to nicked circular form (NC, Form II) without concurrent formation of Form III, revealing the single-strand DNA cleavage. No significant cleavage was found in the presence of Mn1 and Mn2; however, it was observed at 2000 and 3000 mu M concentrations of Fe1. The ability of Fe1 to cleave DNA was greater than that of other complexes and these results are in conformity with their DNA-binding affinities. Cytotoxicity determination tests revealed that the complex Fe1 on HeLa and HT-29 cells exhibited a higher anti-proliferative effect than Mn1 and Mn2 (Fe1>Mn2>Mn1). These studies suggested that the complex Fe1 could be a good candidate as a chemotherapeutic drug targeting DNA. [GRAPHICS]
dc.description.sponsorshipScientific Research Projects Coordination Unit of Istanbul University-Cerrahpasa
dc.description.sponsorshipThis work was supported by the Scientific Research Projects Coordination Unit of Istanbul University-Cerrahpasa. The authors acknowledge Scientific and Technological Research Application and Research Center, Sinop University, Turkey, for the use of the Bruker D8-QUEST diffractometer.
dc.identifier.doi10.1007/s00775-019-01653-6
dc.identifier.endpage376
dc.identifier.issn0949-8257
dc.identifier.issn1432-1327
dc.identifier.issue3
dc.identifier.pmid30895485
dc.identifier.scopus2-s2.0-85063189955
dc.identifier.scopusqualityQ2
dc.identifier.startpage365
dc.identifier.urihttps://doi.org/10.1007/s00775-019-01653-6
dc.identifier.urihttps://hdl.handle.net/11486/6967
dc.identifier.volume24
dc.identifier.wosWOS:000466887300006
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherSpringer
dc.relation.ispartofJournal of Biological Inorganic Chemistry
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WOS_20250323
dc.subjectThiosemicarbazone
dc.subjectIron
dc.subjectManganese
dc.subjectDNA binding
dc.subjectDNA cleavage
dc.subjectAnti-proliferation
dc.titleStructural analysis and biological functionalities of iron(III)- and manganese(III)-thiosemicarbazone complexes: in vitro anti-proliferative activity on human cancer cells, DNA binding and cleavage studies
dc.typeArticle

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