Synthesis, characterization, biological activity and electrochemistry studies of iron(III) complex with curcumin-oxime ligand

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dc.contributor.authorOzbolat, Guluzar
dc.contributor.authorYegani, Arash Alizadeh
dc.date.accessioned2025-03-23T19:32:04Z
dc.date.available2025-03-23T19:32:04Z
dc.date.issued2020
dc.departmentSinop Üniversitesi
dc.description.abstractIron overload is a key target in drug development. This study aimed to investigate the coordination of Fe(III) ions with a curcumin-oxime ligand that may be used in the treatment of iron overload. The synthesis of the curcumin-oxime ligand and curcumin-oxime-Fe(III) complex was successfully made and characterized in its solid-state and solution-state using FT-IR, UV-Vis, elemental analysis, and H-1-NMR. However, in this study, we investigated the apoptotic effects of the curcumin-oxime Fe (III) complex on SW480. SW480 cells were exposed to 99.2% medium for 48 hours. After 48 hours, the incubation period, cells were harvested by centrifugation and washed in phosphate-buffered saline (PBS) and lysed in radio-immunoprecipitation assay (RIPA) buffer for 20 minutes and supernatants were taken and pellets were discarded. ELISA test was used to examine the expression, and activity of cleaved caspase-3, Bax, and Bcl-2 proteins in SW480 cells. ELISA test results indicated that the activities of apoptotic proteins Bax, caspase 3 and Bcl-2 in human SW480 cell lines significantly increased in 48 hours treatment. Also, the activity of Bcl-2 was observed to decrease significantly. Catalase activities of the complex were investigated. The findings showed that the complex has a catalase activity. The findings suggest that this type of complex may constitute a new and interesting basis for the future search of new and more potent drugs. The SOD activity of the result showed that the complexes possessed a considerable SOD activity with an IC50 value of 7.685 mu M. Also, when compared with the control, a complex increased the SOD levels (P < .05). Electrochemistry studies in the literature have shown that the Fe3+/Fe(2+)couple redox process occurs in low potential. This value is within the range of compounds that are expected to show superoxide dismutase activity. The I-pc/I(pa)shows that one electron transport takes place in the complex. Our results suggest that curcumin-oxime may represent a new approach in the treatment of iron overload.
dc.identifier.doi10.1111/1440-1681.13359
dc.identifier.endpage1842
dc.identifier.issn0305-1870
dc.identifier.issn1440-1681
dc.identifier.issue11
dc.identifier.pmid32497256
dc.identifier.scopus2-s2.0-85089539947
dc.identifier.scopusqualityQ2
dc.identifier.startpage1834
dc.identifier.urihttps://doi.org/10.1111/1440-1681.13359
dc.identifier.urihttps://hdl.handle.net/11486/5405
dc.identifier.volume47
dc.identifier.wosWOS:000563877600001
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherWiley
dc.relation.ispartofClinical and Experimental Pharmacology and Physiology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WOS_20250323
dc.subjectcatalase
dc.subjectcaspase-3
dc.subjectchelation
dc.subjectcurcumin-oxime
dc.subjectiron overload
dc.subjectligand
dc.titleSynthesis, characterization, biological activity and electrochemistry studies of iron(III) complex with curcumin-oxime ligand
dc.typeArticle

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