Interface imprinted polymers with well-oriented recognition sites for selective purification of hemoglobin

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dc.authoridUzun, Lokman/0000-0002-3971-7725
dc.authoridCorman, Mehmet Emin/0000-0001-9062-4201
dc.contributor.authorArmutcu, Canan
dc.contributor.authorOzgur, Erdogan
dc.contributor.authorCorman, M. Emin
dc.contributor.authorUzun, Lokman
dc.date.accessioned2025-03-23T19:41:50Z
dc.date.available2025-03-23T19:41:50Z
dc.date.issued2021
dc.departmentSinop Üniversitesi
dc.description.abstractIn this study, we introduced a new strategy to design interface imprinted polymers for a novel aspect of molecular imprinting technique, utilization of sacrificial metal oxide particles. In the first step, bovine hemoglobin (BHb) was adsorbed on zinc oxide (ZnO) particles, which were then used to synthesize polyacrylic acid-based molecular imprinting membrane by bulk polymerization in the presence of ethylene glycol dimethacrylate as a cross-linking agent. After polymerization terminated, BHb-ZnO particles were removed to leave effective imprint sites onto the bulk polymeric network which is responsible for the formation of template orientation. The characterization of membranes was investigated by using Fourier transform infrared (FTIR), Raman spectroscopy (RS), scanning electron microscopy (SEM), surface area measurements (BET analyses) and thermogravimetric analysis (TGA). The interface molecularly imprinted membranes (iMIMs) have a relatively high specific rebinding capacity of 65.98 mg/g and excellent selectivity towards BHb with a separation factor of 6.78. The equilibrium adsorption isotherms fitted well to Langmuir isotherms (R-2 = 0.9944) and the value of adsorption capability (Q(max)) and equilibrium constant (b) were estimated to be 73.53 mg/g and 1.36 mg/mL for the iMIM, respectively. The kinetics of adsorption fitted best to pseudo-second order (R-2 = 0.9912). The ZnO particles were used not only to ensure the preservation of the imprint cavities in the polymer network but also to lead to high template removal and better rebinding kinetics. This novel design with multiple recognition sites is quite simple and suitable for the separation of biomacromolecules.
dc.description.sponsorshipTurkish Academy of Sciences
dc.description.sponsorshipLU thanks Turkish Academy of Sciences for their invaluable support in respect to The Young Scientists Award Programme, TUBA-GEBIP (2017). The study was partially supported with this award.
dc.identifier.doi10.1016/j.colsurfb.2020.111435
dc.identifier.issn0927-7765
dc.identifier.issn1873-4367
dc.identifier.pmid33142256
dc.identifier.scopus2-s2.0-85094593195
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.1016/j.colsurfb.2020.111435
dc.identifier.urihttps://hdl.handle.net/11486/6660
dc.identifier.volume197
dc.identifier.wosWOS:000602406400008
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherElsevier
dc.relation.ispartofColloids and Surfaces B-Biointerfaces
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WOS_20250323
dc.subjectInterface imprinting
dc.subjectSacrificial material
dc.subjectSelective hemoglobin recognition
dc.subjectWell-oriented cavities
dc.subjectOne-step template removal
dc.titleInterface imprinted polymers with well-oriented recognition sites for selective purification of hemoglobin
dc.typeArticle

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