Investigation of the protective effect of thymoquinone of U87 cells induced by beta-amyloid

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dc.contributor.authorOzbolat, G.
dc.contributor.authorAlizade, A.
dc.date.accessioned2025-03-23T19:26:00Z
dc.date.available2025-03-23T19:26:00Z
dc.date.issued2021
dc.departmentSinop Üniversitesi
dc.description.abstractBACKGROUND: Thymoquinone (TQ) is the active compound extracted from Nigella sativa which has been reported to possess various pharmacological attributes. This study shows that Thymoquinone (TQ) is an Alzheimer's disease (AD) model cell line on amyloid-beta (A beta)-induced U87 (human astrocytoma cell line) in beta-amyloid (A beta)-induced in vitro Alzheimer's Disease (AD) model. AIM: We aimed to investigate the effects on antioxidant and apoptotic pathways. METHODS: In the study three groups were formed, the control group, the A beta group, and the A beta+ TQ group obtained by adding TO to the A beta group. Firstly, the cytotoxic potential of TQ in U87 cells was investigated by the colorimetric MTT (3-4,5-dimethyl-thiazolyl-2,5 diphenyltetrazolium bromide) test. To determine the antioxidant status in the cell line treated with Thymoquinone, to examine the effects of superoxide dismutase (SOD) and catalase (CAT) activities, on apoptosis Caspase-3 protein levels were measured by ELISA method. RESULTS: When compared to the control group, the SOD and CAT levels were significantly decreased in the U87 cell line exposed to A beta; Caspase-3 levels were found to increase significantly. However, application of TQ to the A beta-U87 cell line significantly increased SOD and CAT levels; it was found that it decreased the caspase-3 level. CONCLUSION: In in vitro experiments, we determined that TQ has a protective effect by increasing antioxidant parameters in the amyloid beta-induced cell line
dc.identifier.doi10.4149/BLL_2021_120
dc.identifier.endpage752
dc.identifier.issn0006-9248
dc.identifier.issn1336-0345
dc.identifier.issue10
dc.identifier.pmid34570578
dc.identifier.scopus2-s2.0-85116489347
dc.identifier.scopusqualityQ2
dc.identifier.startpage748
dc.identifier.urihttps://doi.org/10.4149/BLL_2021_120
dc.identifier.urihttps://hdl.handle.net/11486/4603
dc.identifier.volume122
dc.identifier.wosWOS:000755925500011
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherAepress Sro
dc.relation.ispartofBratislava Medical Journal-Bratislavske Lekarske Listy
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_WOS_20250323
dc.subjectthymoquinone
dc.subjectamyloid-beta
dc.subjectU87
dc.subjectSOD
dc.subjectCAT
dc.subjectcaspase-3
dc.titleInvestigation of the protective effect of thymoquinone of U87 cells induced by beta-amyloid
dc.typeArticle

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