Enhanced kinetic performance and stability of catalase immobilized on epoxy-functionalized kaolinite

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dc.contributor.authorErol, Kadir
dc.contributor.authorVeyisoglu, Aysel
dc.contributor.authorTatar, Demet
dc.contributor.authorKocabas, Buket Bulut
dc.contributor.authorAlacabey, Ihsan
dc.contributor.authorGokmese, Ebru
dc.date.accessioned2026-04-25T14:20:05Z
dc.date.available2026-04-25T14:20:05Z
dc.date.issued2026
dc.departmentSinop Üniversitesi
dc.description.abstractThe immobilization of catalase onto stable, reusable supports is crucial for efficient peroxide-based biocatalytic applications. In this study, catalase was immobilized for the first time onto epoxy-functionalized kaolinite particles prepared via surface silanization with (3-glycidyloxypropyl)trimethoxysilane. Structural and surface characterizations confirmed successful organosilane grafting while preserving the layered kaolinite framework. The modified support exhibited rapid enzyme uptake and a high immobilization capacity of approximately 300 mg g(-1). Kinetic analysis showed a substantial decrease in K-m from 57.3 mM (free catalase) to 21.6 mM after immobilization, indicating enhanced substrate affinity. In contrast, V-max decreased due to diffusion limitations typical of heterogeneous systems. Despite this, catalytic efficiency increased nearly 1.8-fold. Moreover, immobilized catalase demonstrated significantly improved operational reusability and long-term storage stability compared to the free enzyme. These results highlight silanized kaolinite as a robust, low-cost, and efficient mineral-based support for catalase immobilization, with strong potential for environmental and industrial biocatalytic applications.
dc.identifier.doi10.1038/s41598-026-38910-z
dc.identifier.issn2045-2322
dc.identifier.issue1
dc.identifier.orcid0000-0002-3080-2296
dc.identifier.pmid41663741
dc.identifier.scopus2-s2.0-105032233437
dc.identifier.scopusqualityN/A
dc.identifier.urihttps://doi.org/10.1038/s41598-026-38910-z
dc.identifier.urihttps://hdl.handle.net/11486/8346
dc.identifier.volume16
dc.identifier.wosWOS:001714885800015
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherNature Portfolio
dc.relation.ispartofScientific Reports
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_WOS_20260420
dc.subjectCatalase
dc.subjectImmobilization
dc.subjectKaolinite
dc.subjectKinetics
dc.subjectSilanization
dc.subjectStability
dc.titleEnhanced kinetic performance and stability of catalase immobilized on epoxy-functionalized kaolinite
dc.typeArticle

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