Isolation and Description of Bacterial Strains from Iron-Enriched Media: Phylogenetic Perspectives, Functional Features, and Hunting for Iron-Oxidizers

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dc.contributor.authorUysal, Burak
dc.contributor.authorAvsar, Cumhur
dc.date.accessioned2025-03-23T19:35:21Z
dc.date.available2025-03-23T19:35:21Z
dc.date.issued2025
dc.departmentSinop Üniversitesi
dc.description.abstractThe main objective of this study was to isolate aerobic neutrophilic iron-oxidizing bacteria on iron-containing media. Of the 51 pure strains of bacteria examined in the study, every isolate had a reduction in NO3-, 34 (68%) had siderophores in the range of 0-101.2 +/- 11.1 mu mol, 16 (31.3%) had biofilm (two strains with high biofilm), and 10 (19.6%) had phosphate-solubilizing activity. Acinetobacter, Klebsiella, and Serratia species exhibited a significant degree of resemblance; however, the majority of the strains examined, as determined by 16S rRNA sequence analysis, belonged to the Bacillus genus. Furthermore, using the BstNI enzyme for AFLP analysis, 47 strains were discovered to exhibit significant diversity and more than 50% similarity. Moreover, it was discovered that 20 of the strains examined may cause corrosion on Winogradsky medium. The dried and powdered corrosion samples' FTIR spectra were strikingly comparable to those of Fe(OH)3. Although pure iron-oxidizing bacteria were not cultivated, microscopic observations indicated the unique morphologies of Gallionella and Leptothrix. Both isolates were regrettably lost when it was discovered that they could not be maintained under laboratory culture conditions. The ecological functions of bacteria in iron cycling and their possible uses in controlling corrosion and nutrient dynamics are highlighted in this work.
dc.description.sponsorshipTUBITAK 2209-A University Students Research Projects [1919B012338398]
dc.description.sponsorshipThis work was supported by the TUBITAK 2209-A University Students Research Projects support program under grant [1919B012338398].
dc.identifier.doi10.1080/01490451.2025.2462145
dc.identifier.issn0149-0451
dc.identifier.issn1521-0529
dc.identifier.scopus2-s2.0-85218838326
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.1080/01490451.2025.2462145
dc.identifier.urihttps://hdl.handle.net/11486/5849
dc.identifier.wosWOS:001419230000001
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherTaylor & Francis Inc
dc.relation.ispartofGeomicrobiology Journal
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WOS_20250323
dc.subjectIron-oxidizing bacteria
dc.subjectGallionella
dc.subjectLeptothrix
dc.subjectcorrosion
dc.subjectFTIR
dc.titleIsolation and Description of Bacterial Strains from Iron-Enriched Media: Phylogenetic Perspectives, Functional Features, and Hunting for Iron-Oxidizers
dc.typeArticle

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