Isolation and Description of Bacterial Strains from Iron-Enriched Media: Phylogenetic Perspectives, Functional Features, and Hunting for Iron-Oxidizers
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Tarih
2025
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Taylor & Francis Inc
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
The main objective of this study was to isolate aerobic neutrophilic iron-oxidizing bacteria on iron-containing media. Of the 51 pure strains of bacteria examined in the study, every isolate had a reduction in NO3-, 34 (68%) had siderophores in the range of 0-101.2 +/- 11.1 mu mol, 16 (31.3%) had biofilm (two strains with high biofilm), and 10 (19.6%) had phosphate-solubilizing activity. Acinetobacter, Klebsiella, and Serratia species exhibited a significant degree of resemblance; however, the majority of the strains examined, as determined by 16S rRNA sequence analysis, belonged to the Bacillus genus. Furthermore, using the BstNI enzyme for AFLP analysis, 47 strains were discovered to exhibit significant diversity and more than 50% similarity. Moreover, it was discovered that 20 of the strains examined may cause corrosion on Winogradsky medium. The dried and powdered corrosion samples' FTIR spectra were strikingly comparable to those of Fe(OH)3. Although pure iron-oxidizing bacteria were not cultivated, microscopic observations indicated the unique morphologies of Gallionella and Leptothrix. Both isolates were regrettably lost when it was discovered that they could not be maintained under laboratory culture conditions. The ecological functions of bacteria in iron cycling and their possible uses in controlling corrosion and nutrient dynamics are highlighted in this work.
Açıklama
Anahtar Kelimeler
Iron-oxidizing bacteria, Gallionella, Leptothrix, corrosion, FTIR
Kaynak
Geomicrobiology Journal
WoS Q Değeri
Q2
Scopus Q Değeri
Q1
