A novel Lactiplantibacillus plantarum strain: probiotic properties and optimization of the growth conditions by response surface methodology

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dc.authoridAslan, Emek/0000-0002-1287-3322
dc.authoridDUZEL, Ahmet/0000-0003-4106-4548
dc.authoridGOKMEN, GOKHAN GURUR/0000-0002-1180-0682
dc.authoridSARGIN, SAIT/0000-0002-5037-3193
dc.authoridGoksungur, Yekta/0000-0002-5166-6561
dc.authoridKISLA, Duygu/0000-0002-6993-3883
dc.contributor.authorGokmen, Goekhan Gurur
dc.contributor.authorSariyildiz, Seda
dc.contributor.authorCholakov, Remzi
dc.contributor.authorNalbantsoy, Ayse
dc.contributor.authorBaler, Biray
dc.contributor.authorAslan, Emek
dc.contributor.authorDuzel, Ahmet
dc.date.accessioned2025-03-23T19:44:00Z
dc.date.available2025-03-23T19:44:00Z
dc.date.issued2024
dc.departmentSinop Üniversitesi
dc.description.abstractThe objective of this study is to explore the probiotic properties and optimal growth conditions of Lactiplantibacillus plantarum BG24. L. plantarum BG24 exhibited a remarkable ability to utilize lactose, and to grow under acidic conditions and in the presence of high levels of bile salts. The strain showed the highest antibacterial activity against L. monocytogenes Scott A (zone of inhibition: 26 mm). L. plantarum BG24 was found to be resistant to 8 of the tested 19 antibiotics using the disc diffusion method.and its multiple antibiotic resistance (MAR) index was calculated as 0.421. The adhesion rate to human intestinal epithelial Caco-2 cells was determined as 37.51%. The enzyme profile of L. plantarum BG24 was investigated using API ZYM test kit and the highest enzymatic activities were found for Leucine arylamidase, beta-glucosidase, Valine arylamidase, beta-galactosidase and N-acetyl-beta-glucosaminidase. L. plantarum BG24 strain showed higher microbial growth under static conditions (6.60 OD600) compared to 100 rpm (5.73 OD600) and 200 rpm (5.02 OD600) shaking speed due to its facultative anaerobic characteristic. However, different inoculation rates and glucose addition did not make a statistically significant difference on biomass formation (p > 0.05). The specific growth rate of L. plantarum BG24 was 0.416 h(-1), the doubling time was 1.67 h, and the biomass productivity value was 0.14 gL(-1) h(-1) in the original MRS broth (pH 5.7) while higher values were found as 0.483 h(-1), 1.43 h and 0.17 gL(-1) h(-1), respectively, in MRS broth (pH 6.5) medium enriched with 5 g/L yeast extract. The stirred tank bioreactor was used to optimise the growth of BG24 strain. The process variables was optimized at 0.05 vvm of aeration rate, 479 rpm of agitation speed, 3% of inoculation rate and 18 h of incubation time. The maximum biomass (g/L) production was obtained as 3.84 g/L at the optimized conditions.
dc.description.sponsorshipEge University Research Foundation
dc.description.sponsorshipNo Statement Available
dc.identifier.doi10.1007/s11274-023-03862-3
dc.identifier.issn0959-3993
dc.identifier.issn1573-0972
dc.identifier.issue2
dc.identifier.pmid38194015
dc.identifier.scopus2-s2.0-85181720038
dc.identifier.scopusqualityQ2
dc.identifier.urihttps://doi.org/10.1007/s11274-023-03862-3
dc.identifier.urihttps://hdl.handle.net/11486/6844
dc.identifier.volume40
dc.identifier.wosWOS:001138441700001
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherSpringer
dc.relation.ispartofWorld Journal of Microbiology & Biotechnology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_WOS_20250323
dc.subjectProbiotic
dc.subjectLactiplantibacillus plantarum
dc.subjectBiomass production
dc.subjectResponse surface methodology
dc.titleA novel Lactiplantibacillus plantarum strain: probiotic properties and optimization of the growth conditions by response surface methodology
dc.typeArticle

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