Detection of multidrug-resistant Pseudomonas isolates and distribution of denitrifying functional genes

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dc.authoridAvsar, cumhur/0000-0002-4095-0022
dc.contributor.authorOnal, Nur
dc.contributor.authorAvsar, Cumhur
dc.contributor.authorAras, E. SumerSumer
dc.date.accessioned2025-03-23T19:35:11Z
dc.date.available2025-03-23T19:35:11Z
dc.date.issued2021
dc.departmentSinop Üniversitesi
dc.description.abstractThe aim of this study was to characterize 59 Pseudomonas strains isolated from soil samples in terms of Inducible Beta-Lactamase (IBL), fluorescence production, antibiotic resistance, presence of plasmids and genetic diversity, as well as denitrification functional genes. Fourteen out of fifty-nine (23.7%) Pseudomonas isolates were identified as multidrug-resistant. To evaluate the contribution of denitrification functional genes to genetic diversity, PCR products were screened by RFLP. It was determined that the 18, 6 and 22 out of 59 isolate harbored nirS, nirK and nosZ genes, respectively. It was found that the 37 isolates were nosZ-negative. Thus, these results suggest that nosZ gene-missing pseudomonad denitrifiers are partly contribute to N2O emission. Moreover, nirS, nirK and nosZ genes were found to be positive with IBL and negatively correlated with fluorescence production. These results suggest that Pseudomonas species have important roles in soil and even in biosphere due to their diversity and genetic factors.
dc.identifier.doi10.1080/09603123.2020.1720619
dc.identifier.endpage1031
dc.identifier.issn0960-3123
dc.identifier.issn1369-1619
dc.identifier.issue8
dc.identifier.pmid31994901
dc.identifier.scopus2-s2.0-85078471643
dc.identifier.scopusqualityQ1
dc.identifier.startpage1015
dc.identifier.urihttps://doi.org/10.1080/09603123.2020.1720619
dc.identifier.urihttps://hdl.handle.net/11486/5804
dc.identifier.volume31
dc.identifier.wosWOS:000511636200001
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherTaylor & Francis Ltd
dc.relation.ispartofInternational Journal of Environmental Health Research
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WOS_20250323
dc.subjectPseudomonas
dc.subjectMultidrug-resistant
dc.subjectnosZ
dc.subjectnirS
dc.subjectnirK
dc.subjectRFLP
dc.subjectSDS-PAGE
dc.titleDetection of multidrug-resistant Pseudomonas isolates and distribution of denitrifying functional genes
dc.typeArticle

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