Abscisic acid-regulated responses of aba2-1 under osmotic stress: the abscisic acid-inducible antioxidant defence system and reactive oxygen species production

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dc.authoridSECKIN DINLER, BURCU/0000-0001-6289-380X
dc.authoridTURKAN, ISMAIL/0000-0001-9042-6870
dc.contributor.authorOzfidan, C.
dc.contributor.authorTurkan, I.
dc.contributor.authorSekmen, A. H.
dc.contributor.authorSeckin, B.
dc.date.accessioned2025-03-23T19:31:52Z
dc.date.available2025-03-23T19:31:52Z
dc.date.issued2012
dc.departmentSinop Üniversitesi
dc.description.abstractWe investigated the interaction among abscisic acid (ABA), reactive oxygen species (ROS) and antioxidant defence system in the transduction of osmotic stress signalling using Arabidopsis thaliana WT (Columbia ecotype, WT) and an ABA-deficient mutant (aba2-1). For this, 50 mu m ABA and osmotic stress, induced with 40% (w/v) polyethylene glycol (PEG8000; -0.7 MPa), were applied to WT and aba2-1 for 6, 12 or 24 h. Time course analysis was undertaken for determination of total/isoenzyme activity of the antioxidant enzymes, superoxide dismutase (SOD; EC 1.15.1.1), catalase (CAT; EC 1.11.1.6), ascorbate peroxidase (APX; EC 1.11.1.11), NADPH oxidase (NOX; EC 1.6.3.1) activity; scavenging activity of the hydroxyl radical (OH?), hydrogen peroxide (H2O2); endogenous ABA and malondialdehyde (MDA). The highest H2O2 and MDA content was found in PEG-treated groups of both genotypes, but with more in aba2-1. ABA treatment under stress reduced the accumulation of H2O2 and MDA, while it promoted activity of SOD, CAT and APX. APX activity was higher than CAT activity in ABA-treated WT and aba2-1, indicating a protective role of APX rather than CAT during osmotic stress-induced oxidative damage. Treatment with ABA also significantly induced increased NOX activity. Oxidative damage was lower in ABA-treated seedlings of both genotypes, which was associated with greater activity of SOD (Mn-SOD1 and 2 and Fe-SOD isoenzymes), CAT and APX in these seedlings after 24 h of stress. These results suggest that osmotic stress effects were overcome by ABA treatment because of increased SOD, CAT, APX and NOX.
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TUBITAK) [TBAG 107T671]; Ege University Science and Technology Center (EBILTEM) [2008-BIL/007]
dc.description.sponsorshipFinancial support for this work was provided by the Scientific and Technological Research Council of Turkey (TUBITAK; Project number: TBAG 107T671) and Ege University Science and Technology Center (EBILTEM; Project number, 2008-BIL/007). The authors express great gratitude to Prof. Dr Laszlo Szabados, Biological Research Center of the Hungarian Academy of Sciences, Szeged, Hungary, for providing the ABA-deficient mutant (aba2-1) of A. thaliana. The authors also thank Dr Ayse Nalbantsoy, Ege University, for help with ABA analyses.
dc.identifier.doi10.1111/j.1438-8677.2011.00496.x
dc.identifier.endpage346
dc.identifier.issn1435-8603
dc.identifier.issn1438-8677
dc.identifier.issue2
dc.identifier.pmid21973087
dc.identifier.scopus2-s2.0-84856696275
dc.identifier.scopusqualityQ1
dc.identifier.startpage337
dc.identifier.urihttps://doi.org/10.1111/j.1438-8677.2011.00496.x
dc.identifier.urihttps://hdl.handle.net/11486/5382
dc.identifier.volume14
dc.identifier.wosWOS:000299928900010
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherWiley
dc.relation.ispartofPlant Biology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WOS_20250323
dc.subjectaba2-1
dc.subjectABA-deficient mutant
dc.subjectabscisic acid
dc.subjectantioxidant enzymes
dc.subjectosmotic stress
dc.subjectreactive oxygen species
dc.titleAbscisic acid-regulated responses of aba2-1 under osmotic stress: the abscisic acid-inducible antioxidant defence system and reactive oxygen species production
dc.typeArticle

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