Detection of Cryptosporidium Species in the Sea and Tap Water Samples of Black Sea, Turkey

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dc.authoridAvsar, cumhur/0000-0002-4095-0022
dc.contributor.authorKoloren, Zeynep
dc.contributor.authorKaya, Derya
dc.contributor.authorAvsar, Cumhur
dc.date.accessioned2025-03-23T19:30:02Z
dc.date.available2025-03-23T19:30:02Z
dc.date.issued2013
dc.departmentSinop Üniversitesi
dc.description.abstractThe aim of this study was to evaluate Cryptosporidium spp. contamination of sea and tap water samples from Sinop and Ordu Provinces, Black Sea, Turkey. The samples (10 L) were collected in spring, summer, autumn, and winter in 2011. A total of 128 water samples was analyzed using an immunofluorescence test (IFT), as well as loop-mediated isothermal amplification (LAMP) and nested polymerase chain reaction (PCR). Cryptosporidium spp. oocysts were detected by IFT in 43 of the 70 samples (61.4%; 1-40 oocysts per 0.5 L) and 35 of the 58 samples (60.3%; 1-23 oocysts per 0.5 L) in the sea water samples from Ordu and Sinop, respectively. The highest number of oocysts by IFT were detected in spring and winter in Ordu and Sinop, respectively. The results of the S-adenosylmethionine synthetase (SAM) gene LAMP assays were 65.5% positive for Cryptosporidium parvum, Cryptosporidium hominis, and Cryptosporidium meleagridis in all examined samples, while the SSUrRNA gene nested PCR assay was 31.0% positive. Six C. parvum nested PCR products from all positive samples were successfully sequenced.
dc.identifier.doi10.1645/GE-3232.1
dc.identifier.endpage557
dc.identifier.issn0022-3395
dc.identifier.issn1937-2345
dc.identifier.issue3
dc.identifier.pmid23145771
dc.identifier.scopusqualityQ3
dc.identifier.startpage554
dc.identifier.urihttps://doi.org/10.1645/GE-3232.1
dc.identifier.urihttps://hdl.handle.net/11486/4991
dc.identifier.volume99
dc.identifier.wosWOS:000322521800034
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherAllen Press Inc
dc.relation.ispartofJournal of Parasitology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WOS_20250323
dc.subjectMediated Isothermal Amplification
dc.subjectPolymerase-Chain-Reaction
dc.subjectGiardia Cysts
dc.subjectToxoplasma-Gondii
dc.subjectWaste-Water
dc.subjectOocysts
dc.subjectSupplies
dc.subjectRaw
dc.subjectPcr
dc.subjectDna
dc.titleDetection of Cryptosporidium Species in the Sea and Tap Water Samples of Black Sea, Turkey
dc.typeArticle

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