Detection of Cryptosporidium Species in the Sea and Tap Water Samples of Black Sea, Turkey
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Tarih
2013
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Allen Press Inc
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
The aim of this study was to evaluate Cryptosporidium spp. contamination of sea and tap water samples from Sinop and Ordu Provinces, Black Sea, Turkey. The samples (10 L) were collected in spring, summer, autumn, and winter in 2011. A total of 128 water samples was analyzed using an immunofluorescence test (IFT), as well as loop-mediated isothermal amplification (LAMP) and nested polymerase chain reaction (PCR). Cryptosporidium spp. oocysts were detected by IFT in 43 of the 70 samples (61.4%; 1-40 oocysts per 0.5 L) and 35 of the 58 samples (60.3%; 1-23 oocysts per 0.5 L) in the sea water samples from Ordu and Sinop, respectively. The highest number of oocysts by IFT were detected in spring and winter in Ordu and Sinop, respectively. The results of the S-adenosylmethionine synthetase (SAM) gene LAMP assays were 65.5% positive for Cryptosporidium parvum, Cryptosporidium hominis, and Cryptosporidium meleagridis in all examined samples, while the SSUrRNA gene nested PCR assay was 31.0% positive. Six C. parvum nested PCR products from all positive samples were successfully sequenced.
Açıklama
Anahtar Kelimeler
Mediated Isothermal Amplification, Polymerase-Chain-Reaction, Giardia Cysts, Toxoplasma-Gondii, Waste-Water, Oocysts, Supplies, Raw, Pcr, Dna
Kaynak
Journal of Parasitology
WoS Q Değeri
Q4
Scopus Q Değeri
Q3
Cilt
99
Sayı
3
