Expression profiles of proto-oncogene TWIST1 and tumor metastasis suppressor gene LASS2 in bladder cancer

Basit Öğe Kaydı
dc.contributor.authorYegin, Zeynep
dc.contributor.authorAydin, Oguz
dc.contributor.authorKoc, Haydar
dc.contributor.authorBuyukalpelli, Recep
dc.date.accessioned2025-03-23T19:30:30Z
dc.date.available2025-03-23T19:30:30Z
dc.date.issued2018
dc.departmentSinop Üniversitesi
dc.description.abstractTranscription factor proto-oncogene TWIST1 and tumor metastasis suppressor gene LASS2 have been reported to be involved in various carcinomas but their expression profiles and prognostic significances in bladder cancer are largely unknown. We aimed to determine these genes' expression levels both at mRNA and protein level in bladder cancer. mRNA expression levels of TWIST1 and LASS2 genes were examined using real-time quantitative PCR (qPCR) in human bladder tumors and paired normal adjacent tissues obtained from 44 patients. Protein expression profiles of both genes were detected by immunohistochemical staining in formalin-fixed and paraffin-embedded tissues from the same patients. The expression profiles of TWIST1 mRNA in bladder tumors were significantly lower than the normal adjacent tissues and linked to both the stage and the grade. The expression profiles of LASS2 mRNA in bladder tumors were also significantly lower than the normal adjacent tissues reflecting the potential tumor suppressor profile of the gene, independently from stage or grade. By immunohistochemistry, TWIST1 and LASS2 positive expression rates were found as 14.3% (6/42) and 38.1% (16/42), respectively. As potential molecular markers for bladder carcinoma, both TWIST1 and LASS2 transcripts seem to play role during the tumorigenesis and development of bladder cancer. Lack of a functional link and/or weak inverse link between TWIST1 and LASS2 transcripts and immunohistochemical findings may reflect the potential associations of transcription regulation mechanisms and merit further investigations. To the best of our knowledge, this is the first report investigating the combined expression profile of TWIST1 and LASS2 in bladder cancer both at mRNA and protein level.
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TUBITAK) [114S417]
dc.description.sponsorshipThe authors acknowledge Sinop University, Scientific and Technological Research Application and Research Center (SUBITAM) (Dedicated to the precious memory of Professor Dr. Ismet Berber) and Ondokuz Mayis University Advanced Technology Research and Application Center (KITAM) (Very special thanks to Dr. Asli Dageri) for scientific collaboration. This study was supported by the Scientific and Technological Research Council of Turkey (TUBITAK) (Project no: 114S417).
dc.identifier.doi10.14715/cmb/2018.64.11.12
dc.identifier.endpage73
dc.identifier.issn0145-5680
dc.identifier.issn1165-158X
dc.identifier.issue11
dc.identifier.pmid30213291
dc.identifier.scopus2-s2.0-85056807987
dc.identifier.scopusqualityQ4
dc.identifier.startpage66
dc.identifier.urihttps://doi.org/10.14715/cmb/2018.64.11.12
dc.identifier.urihttps://hdl.handle.net/11486/5085
dc.identifier.volume64
dc.identifier.wosWOS:000444259700012
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherC M B Assoc
dc.relation.ispartofCellular and Molecular Biology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WOS_20250323
dc.subjectBladder cancer
dc.subjectTWIST1
dc.subjectLASS2
dc.subjectGene expression
dc.subjectImmunohistochemistry
dc.titleExpression profiles of proto-oncogene TWIST1 and tumor metastasis suppressor gene LASS2 in bladder cancer
dc.typeArticle

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
PubMed İndeksli Yayınlar Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu