Predictive value of KRAS/NRAS, IL-4 VNTR and HPV alterations in metastases of colorectal cancer

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dc.authoridAvsar, cumhur/0000-0002-4095-0022
dc.contributor.authorFiliz, Ozen
dc.contributor.authorZeynep, Yegin
dc.contributor.authorCumhur, Avsar
dc.contributor.authorTulay, Zenginkinet
dc.contributor.authorHaydar, Koc
dc.contributor.authorCelal, Ulasoglu
dc.date.accessioned2025-03-23T19:26:00Z
dc.date.available2025-03-23T19:26:00Z
dc.date.issued2022
dc.departmentSinop Üniversitesi
dc.description.abstractPURPOSE: The main aim of the study was to evaluate the potential roles of KRAS/NRAS proto-oncogenes, IL-4 VNTR variants and HPV prevalence in colorectal cancer metastasis. As the second aim, the interactions of the analyzed genes and viral sequences with both clinicopathological variables and each other were targeted.METHODS: DNA was extracted using AmoyDx FFPE DNA Extraction kit from paraffin-embedded colorectal tumor tissue samples (n = 60). NRAS/KRAS mutational profiles were determined with real-time polymerase chain reaction using AmoyDx KRAS/NRAS Mutation Detection Kit. Genotyping of IL-4 VNTR was made with PCR. HPV detection was analyzed by PCR with both GP5+/GP6+ consensus primers and type-specific primers for HPV-16 and HPV-18. SPSS v22 (IBM) statistics software was used for all statistical analyses.RESULTS: From the demographical/clinicopathological parameters, age and biopsy specimens revealed an association with metastasis. KRAS mutation rate was as high as 65 % in the patients and the most prevalent mutation type was G12D. Metastasis risk was 3.19-fold increased in KRAS-mutated patients compared to KRAS-negative ones. IL-4 VNTR genotypes/alleles were not associated with metastasis in our analysis. The frequency of HPVs in our colorectal cancer cohort was 36.7 %, but HPV positivity was not found to be associated with metastasis. A significant association was found between HPV and NRAS mutations; NRAS wild-type status acted as a protective factor by 7.5-fold for HPV negativity.CONCLUSION: Our study comprehensively and concomitantly evaluated several potential molecular risk factors. Future studies designed in such combined approaches will substantially contribute to better manage colorectal cancer tumorigenesis from molecular biological perspective (Tab. 6, Fig. 2, Ref. 40). Text in PDF www.elis.sk.
dc.identifier.doi10.4149/BLL_2022_122
dc.identifier.endpage768
dc.identifier.issn0006-9248
dc.identifier.issn1336-0345
dc.identifier.issue10
dc.identifier.pmid35913013
dc.identifier.scopus2-s2.0-85138205894
dc.identifier.scopusqualityQ2
dc.identifier.startpage758
dc.identifier.urihttps://doi.org/10.4149/BLL_2022_122
dc.identifier.urihttps://hdl.handle.net/11486/4602
dc.identifier.volume123
dc.identifier.wosWOS:000858637000012
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherAepress Sro
dc.relation.ispartofBratislava Medical Journal-Bratislavske Lekarske Listy
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_WOS_20250323
dc.subjectcolorectal cancer
dc.subjectKRAS
dc.subjectNRAS
dc.subjectIL-4
dc.subjecthuman papillomavirus
dc.subjectmetastasis
dc.titlePredictive value of KRAS/NRAS, IL-4 VNTR and HPV alterations in metastases of colorectal cancer
dc.typeArticle

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