Community structures and comparison of nosZ and 16S rRNA genes from culturable denitrifying bacteria

Basit Öğe Kaydı
dc.authoridAvsar, cumhur/0000-0002-4095-0022
dc.contributor.authorAvsar, Cumhur
dc.contributor.authorAras, E. Sumer
dc.date.accessioned2025-03-23T19:42:25Z
dc.date.available2025-03-23T19:42:25Z
dc.date.issued2020
dc.departmentSinop Üniversitesi
dc.description.abstractThe objectives of this study were (i) to isolate and characterize of cultivable denitrifying bacteria using classic microbiological and molecular methods, (ii) to compare of 16S rRNA and nosZ genes as molecular markers, (iii) to determine bacterial community structure and diversity in soil samples using single-strand conformation polymorphism (SSCP) analysis. In this study, 49 bacterial isolates were cultivated and phylogenetic analyses grouped them into two phyla: Proteobacteria (37 species) and Firmicutes (12 species). Our study showed that the nosZ functional gen could be used to identify denitrifying bacteria abundance in environment but could not be used to identify pure bacterial cultures. In addition, the bacterial community structure showed significant differences among the various soil types. Phylogenetic analysis of community structure indicated that 51 clones could be divided into 2 phylotypes. Uncultured bacteria (80.4%) and Gammaproteobacteria (19.6%) were the dominant components of the soil bacterial community. For 16S rRNA, PCR products of 49 bacteria were obtained with 27F-1492R primer pairs. For nosZ, PCR products were obtained with primers 1F-1R (259 bp), 2F-2R (267 bp), and F-1622R (453 bp) of 39 bacteria that the single nosZ band provided on the agarose gel. The bacterial 16S rRNA gene clone library was dominated by Gammaproteobacteria and Bacilli. The nosZ clone sequences did not represent the bacteria from which they were obtained but were found to be closer to the environmental clones. Our study showed that the nosZ functional gene could be used to identify denitrification abundance in environment but could not be used to identify pure bacterial cultures. It was also found that the nosZ sequences showed uncultured denitrifier species.
dc.description.sponsorshipAnkara University Scientific Research Project Coordination Unit [17L0430004]
dc.description.sponsorshipThis research has been supported by Ankara University Scientific Research Project Coordination Unit. Project Number: 17L0430004, 2017-2018.
dc.identifier.doi10.1007/s12223-019-00754-8
dc.identifier.endpage510
dc.identifier.issn0015-5632
dc.identifier.issn1874-9356
dc.identifier.issue3
dc.identifier.pmid31677094
dc.identifier.scopus2-s2.0-85074835801
dc.identifier.scopusqualityQ2
dc.identifier.startpage497
dc.identifier.urihttps://doi.org/10.1007/s12223-019-00754-8
dc.identifier.urihttps://hdl.handle.net/11486/6788
dc.identifier.volume65
dc.identifier.wosWOS:000493629100001
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherSpringer
dc.relation.ispartofFolia Microbiologica
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WOS_20250323
dc.subjectNitrous-Oxide Reductase
dc.subjectStrand-Conformation Polymorphism
dc.subjectMicrobial Community
dc.subjectMolecular Methods
dc.subjectN2o Emissions
dc.subjectDiversity
dc.subjectSoil
dc.subjectDenitrification
dc.subjectIdentification
dc.subjectSscp
dc.titleCommunity structures and comparison of nosZ and 16S rRNA genes from culturable denitrifying bacteria
dc.typeArticle

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
PubMed İndeksli Yayınlar Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu