Expression of GRP78 and its copartners in HEK293 and pancreatic cancer cell lines (BxPC-3/PANC-1) exposed to MRI and CT contrast agents

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dc.authoridDusak, Abdurrahim/0000-0002-4363-5861
dc.authoridazzawri, Ali/0000-0001-5311-8887
dc.contributor.authorAzzawri, Ali Ahmed
dc.contributor.authorYildirim, Ibrahim Halil
dc.contributor.authorYegin, Zeynep
dc.contributor.authorDusak, Abdurrahim
dc.date.accessioned2025-03-23T19:34:47Z
dc.date.available2025-03-23T19:34:47Z
dc.date.issued2024
dc.departmentSinop Üniversitesi
dc.description.abstractEndoplasmic reticulum (ER) stress-associated chaperones trigger a defense mechanism called as unfolded protein response (UPR) which can manage apoptosis and be determinative in cell fate. Both anticancer drug effects and potential toxicity effects of magnetic resonance imaging (MRI) and computed tomography (CT) contrast agents were aimed to be evaluated. For this purpose, we investigated expression profiles of endoplasmic reticulum stress-associated chaperone molecules in human pancreatic tumor lines BxPC-3 and PANC-1 and control human embryonic kidney cells 293 (HEK293) induced with a variety of gadolinium and iohexol contrast agents. Protein expression levels of ER stress-associated chaperones (master regulator: GRP78/Bip and its copartners: Calnexin, Ero1, PDI, CHOP, IRE1 alpha and PERK) were evaluated with Western blotting. Expression levels at mRNA level were also assessed for GRP78/Bip and CHOP with real-time PCR. Induction of cells was carried out with four different Gd-based contrast agents (GBCAs): (Dotarem, Optimark, Primovist and Gadovist) and two different iohexol agents (Omnipol, Omnipaque). CT contrast agents tested in the study did not result in significant ER stress in HEK293 cells. However, they do not seem to have theranostic potential in pancreas cancer through ER pathway. The potential efficiency of macrocyclic MRI contrast agents to provoke apoptosis via ER stress-associated chaperones in BxPC-3 cells lends credibility for their future theranostic use in pancreas cancer as long as undesired toxicity effects were carefully considered. ER stress markers and/or contrast agents seem to have promising potential to be translated into the clinical practice to manage pancreas cancer progression.
dc.identifier.doi10.1080/15257770.2023.2263496
dc.identifier.endpage416
dc.identifier.issn1525-7770
dc.identifier.issn1532-2335
dc.identifier.issue5
dc.identifier.pmid37787049
dc.identifier.scopus2-s2.0-85173550564
dc.identifier.scopusqualityQ4
dc.identifier.startpage391
dc.identifier.urihttps://doi.org/10.1080/15257770.2023.2263496
dc.identifier.urihttps://hdl.handle.net/11486/5734
dc.identifier.volume43
dc.identifier.wosWOS:001075541200001
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherTaylor & Francis Inc
dc.relation.ispartofNucleosides Nucleotides & Nucleic Acids
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WOS_20250323
dc.subjectPancreatic cancer
dc.subjectendoplasmic reticulum stress
dc.subjectunfolded protein response
dc.subjectGRP78/bip
dc.subjectgadolinium
dc.subjectiohexol
dc.titleExpression of GRP78 and its copartners in HEK293 and pancreatic cancer cell lines (BxPC-3/PANC-1) exposed to MRI and CT contrast agents
dc.typeArticle

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