Glucose biosensor based on immobilization of glucose oxidase on a carbon paste electrode modified with microsphere-attached L-glycine

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dc.authoridSARI, NURSEN/0000-0002-4764-7747
dc.authoridARSLAN, Halit/0000-0002-8275-0002
dc.authoridHASANOGLU OZKAN, ELVAN/0000-0001-7338-4015
dc.authoridDonmez, Soner/0000-0003-0328-6481
dc.contributor.authorDonmez, Soner
dc.contributor.authorArslan, Fatma
dc.contributor.authorSari, Nursen
dc.contributor.authorOzkan, Elvan Hasanoglu
dc.contributor.authorArslan, Halit
dc.date.accessioned2025-03-23T19:47:15Z
dc.date.available2025-03-23T19:47:15Z
dc.date.issued2017
dc.departmentSinop Üniversitesi
dc.description.abstractIn the present study, a novel biosensor that is sensitive to glucose was prepared using the microspheres modified with (4-formyl-3-methoxyphenoxymethyl) polystyrene (FMPS) with L-glycine. Polymeric microspheres having Schiff bases were prepared from FMPS using the glycine condensation method. Glucose oxidase enzyme was immobilized onto modified carbon paste electrode by cross-linking with glutaraldehyde. Oxidation of enzymatically produced H2O2 (+0.5 V vs. Ag/AgCl) was used for determination of glucose. Optimal temperature and pH were found as 50 degrees C and 8.0, respectively. The glucose biosensor showed a linear working range from 5.0 x 10(-4) to 1.0 x 10(-2) M, R-2 = 0.999. Storage and operational stability of the biosensor were also investigated. The biosensor gave perfect reproducible results after 20 measurements with 3.3% relative standard deviation. It also had good storage stability. (C) 2016 International Union of Biochemistry and Molecular Biology, Inc.
dc.identifier.doi10.1002/bab.1533
dc.identifier.endpage753
dc.identifier.issn0885-4513
dc.identifier.issn1470-8744
dc.identifier.issue5
dc.identifier.pmid27629272
dc.identifier.scopus2-s2.0-85018647138
dc.identifier.scopusqualityQ2
dc.identifier.startpage745
dc.identifier.urihttps://doi.org/10.1002/bab.1533
dc.identifier.urihttps://hdl.handle.net/11486/7320
dc.identifier.volume64
dc.identifier.wosWOS:000423086600015
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherWiley
dc.relation.ispartofBiotechnology and Applied Biochemistry
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WOS_20250323
dc.subjectbiosensor
dc.subjectcarbon paste
dc.subjectglucose oxidase
dc.subjectmicrosphere
dc.subjectpolystyrene
dc.subjectSchiff base
dc.titleGlucose biosensor based on immobilization of glucose oxidase on a carbon paste electrode modified with microsphere-attached L-glycine
dc.typeArticle

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