Quantification of denitrifier genes population size and its relationship with environmental factors

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dc.authoridAvsar, cumhur/0000-0002-4095-0022
dc.contributor.authorAvsar, Cumhur
dc.contributor.authorAras, E. Sumer
dc.date.accessioned2025-03-23T19:44:41Z
dc.date.available2025-03-23T19:44:41Z
dc.date.issued2020
dc.departmentSinop Üniversitesi
dc.description.abstractThe objectives of this study were to use real-time PCR for culture-independent quantification of the copy numbers of 16S rRNA and denitrification functional genes, and also the relationships between gene copy numbers and soil physicochemical properties. In this study, qPCR analysis of the soil samples showed 16S rRNA, nirS, nirK, nosZI and nosZII average densities of 3.0 x 10(8), 2.25 x 10(7), 2.9 x 10(5), 4.0 x 10(6) and 1.75 x 10(7) copies per gram of dry soil, respectively. In addition, the abundances of (nirS + nirK), nosZI and nosZII relative to 16S rRNA genes were 1.4-34.1%, 0.06-3.95% and 1.3-39%, respectively, confirming the low proportion of denitrifiers to total bacteria in soil. This showed that the non-denitrifying nosZII-type bacteria may contribute significantly to N2O consumption in the soil. Furthermore, the shifts in abundance and diversity of the total bacteria and denitrification functional gene copy numbers correlated significantly with the various soil factors. It is the first study in Turkey about the population size of denitrification functional genes in different soil samples. It also aims to draw attention to nitrous oxide-associated global warming.
dc.description.sponsorshipAnkara University Scientific Research Project Coordination Unit [17L0430004]
dc.description.sponsorshipThis research was supported by Ankara University Scientific Research Project Coordination Unit. Project number: 17L0430004, 2017-2018.
dc.identifier.doi10.1007/s00203-020-01826-x
dc.identifier.endpage1192
dc.identifier.issn0302-8933
dc.identifier.issn1432-072X
dc.identifier.issue5
dc.identifier.pmid32076734
dc.identifier.scopus2-s2.0-85079756410
dc.identifier.scopusqualityQ3
dc.identifier.startpage1181
dc.identifier.urihttps://doi.org/10.1007/s00203-020-01826-x
dc.identifier.urihttps://hdl.handle.net/11486/6995
dc.identifier.volume202
dc.identifier.wosWOS:000516452000001
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherSpringer
dc.relation.ispartofArchives of Microbiology
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WOS_20250323
dc.subjectDenitrification
dc.subjectNitrous oxide
dc.subjectReal-time PCR
dc.subjectGreenhouse effect
dc.subjectSoil
dc.titleQuantification of denitrifier genes population size and its relationship with environmental factors
dc.typeArticle

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