Molecular imprinted nanosensor based on surface plasmon resonance: Application to the sensitive determination of amoxicillin
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Tarih
2014
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Elsevier Science Sa
Erişim Hakkı
info:eu-repo/semantics/closedAccess
Özet
In this report, we developed surface plasmon resonance (SPR) sensor for the sensitive determination of amoxicillin (AMOX) in chicken egg and human plasma. Firstly, the modification of gold surface of SPR chip was performed by allyl mercaptane. Then, AMOX-imprinted poly(2-hydroxyethyl methacrylate-methacryloylamidoglutamic acid) [p(HEMAGA)] nanofilm was generated on the allyl mercaptane modified gold surface. The unmodified and imprinted surfaces were characterized by fourier transform infrared (FTIR) spectroscopy, ellipsometry and contact angle measurements. The developed method was validated according to the ICH guideline. The linearity range and the detection limit were obtained as 0.1-2.0 ng/mL and 0.022 ng/mL, respectively. The developed imprinted nanosensor was applied to the chicken egg and human plasma samples for the determination of AMOX. In addition, isotherm models were applied to data to explain adsorption process. (C) 2014 Elsevier B.V. All rights reserved.
Açıklama
Anahtar Kelimeler
Amoxicillin, SPR, Molecular imprinting, Validation, Nanosensor
Kaynak
Sensors and Actuators B-Chemical
WoS Q Değeri
Q1
Scopus Q Değeri
N/A
Cilt
195
