Yazar "Veyisoglu, Aysel" seçeneğine göre listele

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    A study of three bacteria isolated from marine sediment and description of Micromonospora globispora sp. nov.
    (Elsevier Gmbh, 2019) Carro, Lorena; Veyisoglu, Aysel; Cetin, Demet; Igual, Jose M.; Klenk, Hans-Peter; Trujillo, Martha E.; Sahin, Nevzat
    During a study looking for the isolation of new actinobacteria strains with potential for antibiotic production from deep marine sediment, three strains were collected with a morphology similar to the one described for the Micromonospora genus. A polyphasic study was designed to determine the taxonomic affiliation of the strains S2901(T), S2903, and S2904. All the strains showed chemotaxonomic properties in line with their classification in the genus Micromonospora, meso-diaminopimelic acid in the wall peptidoglycan, a tetrahydrogenated menaquinone with nine isoprene units as major respiratory quinone, iso-C-15:0 and iso-C-16:0 as major fatty acids and diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol as major polar lipids. The 16S rRNA gene sequences of strain S2901(T), S2903, and S2904 showed the highest similarity (99.2%) with the type strain of Micromonospora halophytica DSM 43171(T), forming an independent branch in the phylogenetic gene tree. Their independent position was confirmed with gyrB gene and MLSA phylogenies. Whole genome sequences confirmed by digital DNA-DNA hybridization analysis that the isolates should be assigned to a new species within the genus Micromonospora for which the name Micromonospora globispora sp. nov. (S2901(T), S2903 and S2904) is proposed. (C) 2018 Elsevier GmbH. All rights reserved.
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    Actinobacteria with heavy metal tolerance from the Sinop coast: A molecular characterization approach
    (Ege Univ, Fac Fisheries, 2025) Veyisoglu, Aysel; Tatar, Demet; Tokatli, Ali; Duyar, Hunkar Avni
    This study aimed to isolate and molecularly characterize marine actinobacteria tolerant to heavy metals from sediment samples collected from the Meydankap & imath; coast in Sinop, T & uuml;rkiye. Sediment samples were obtained from three different stations and cultured on five selective media, leading to the isolation of 54 actinobacteria strains. The highest isolation efficiency was achieved on R2A and SM3 media. The tolerance of the isolates to heavy metals, including lead (Pb), copper (Cu), zinc (Zn), mercury (Hg), and cadmium (Cd), was tested. The results revealed that all isolates were sensitive to Hg and Cd but exhibited high tolerance to Pb. Twelve isolates, showing the highest resistance to heavy metals, were selected for genomic DNA isolation. The 16S rRNA gene region was amplified using universal primers and sequenced. Sequence analysis identified the isolates as belonging to the genera Streptomyces, Actinomadura, Nocardia and Nonomuraea. The approximately 1500-bp 16S rRNA gene sequences were compared with the EzTaxon database, and the closest species were determined. The obtained sequences were deposited in the GenBank database. The results indicate that marine actinobacteria isolated from polluted sediments of the Black Sea possess significant potential for bioremediation and environmental biotechnology applications due to their resistance mechanisms to heavy metals.
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    Antimicrobial magnetic poly(GMA) microparticles: synthesis, characterization and lysozyme immobilization
    (Walter De Gruyter Gmbh, 2021) Erol, Kadir; Tatar, Demet; Veyisoglu, Aysel; Tokatli, Ali
    Micron-sized magnetic particles currently find a wide range of applications in many areas including biotechnology, biochemistry, colloid sciences and medicine. In this study, magnetic poly(glycidyl methacrylate) microparticles were synthesized by providing a polymerization around Fe(II)-Ni(II) magnetic double salt. Adsorption of lysozyme protein from aqueous systems was studied with these particles. Adsorption studies were performed with changing pH values, variable amount of adsorbent, different interaction times and lysozyme amounts. The adsorption capacity of the particles was investigated, and a value of about 95.6 mg lysozyme/g microparticle was obtained. The enzyme activity of the immobilized lysozyme was examined and found to be more stable and reusable compared to the free enzyme. The immobilized enzyme still showed 80% activity after five runs and managed to maintain 78% of its initial activity at the end of 60 days. Besides, in the antimicrobial analysis study for six different microorganisms, the minimum inhibitory concentration value of lysozyme immobilized particles was calculated as 125 tg/mL like free lysozyme. Finally, the adsorption interaction was found to be compatible with the Langmuir isotherm model. Accordingly, it can be said that magnetic poly(GMA) microparticles are suitable materials for lysozyme immobilization and immobilized lysozyme can be used in biotechnological studies.
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    Çorum İli Çevresinde Bulunan Tıbbi Öneme Sahip Bazı Bitkilerin Metanol Ekstraktlarının Antimikrobiyal Aktivitelerinin Belirlenmesi
    (2025) Tatar, Demet; Duyar, Hünkar Avni; Veyisoglu, Aysel; Kuruçay, Nermin
    Amaç: Bu çalışmada, Çorum ilinde geleneksel tedavi amacıyla kullanılan on tıbbi bitkinin metanol ekstraktlarının antimikrobiyal aktivitelerinin araştırılması amaçlanmıştır. Materyal ve Yöntem: Bitki örnekleri ilkbahar döneminde toplanmış, kurutulduktan sonra %70 metanol ile ekstraksiyon yapılmıştır. Elde edilen ekstraktlar disk difüzyon yöntemi ile üç Gram-pozitif bakteri (Bacillus subtilis, Staphylococcus aureus, Enterococcus faecalis), üç Gram-negatif bakteri (Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa) ve iki maya-küf türü (Candida albicans, Aspergillus niger) üzerinde test edilmiştir. Pozitif kontroller olarak Ampisilin, Polimiksin B ve Nistatin kullanılmıştır. Araştırma Bulguları: Sonuçlar, dokuz bitki ekstraktının K. pneumoniae üzerinde, tüm ekstraktların ise B. subtilis üzerinde farklı düzeylerde inhibisyon gösterdiğini ortaya koymuştur. Yedi ekstrakt E. faecalis üzerinde etkili olurken, yalnızca meşe palamudu ve iğde ekstraktları A. niger üzerinde sınırlı antifungal aktivite göstermiştir. Buna karşın, S. aureus, E. coli, P. aeruginosa ve C. albicans üzerinde herhangi bir antimikrobiyal etki gözlenmemiştir. Sonuç: Çalışma, Çorum yöresinde kullanılan bazı bitkilerin antimikrobiyal potansiyel taşıdığını göstermektedir. Özellikle B. subtilis ve E. faecalis üzerinde etkili olan ekstraktlar, doğal ürünlerin yeni antimikrobiyal ajanlar geliştirilmesinde değerlendirilebileceğini işaret etmektedir. İlerleyen çalışmalarda bu bitkilerden elde edilen aktif bileşenlerin izolasyonu ve karakterizasyonu önerilmektedir.
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    Design, synthesis and spectroscopic and structural characterization of novel N-(2-hydroxy-5-methyl-phenyl)-2,3-dimethoxybenzamide: DFT, Hirshfeld surface analysis, antimicrobial activity, molecular docking and toxicology
    (Int Union Crystallography, 2022) Cakmak, Sukriye; Aycan, Tugba; Ozturk, Filiz; Veyisoglu, Aysel
    The novel compound N-(2-hydroxy-5-methylphenyl)-2,3-dimethoxybenzamide, C16H17NO4, I, was prepared by a two-step reaction and then characterized by elemental analysis and X-ray diffraction (XRD) methods. Moreover, its spectroscopic properties were investigated by FT-IR and H-1 and C-13 NMR. Compound I crystallized in the monoclinic space group P2(1)/c and the molecular geometry is not planar, being divided into three planar regions. Supramolecular structures are formed by connecting units via hydrogen bonds. The ground-state molecular structure of I was optimized by the DFT-B3LYP/6-31G(d,p) method and the theoretical structure was compared with that obtained by X-ray diffraction. Intermolecular interactions in the crystal network were studied by two-dimensional (2D) and three-dimensional (3D) Hirshfeld analyses. The calculated electronic transition results were examined and the molecular electrostatic potentials (MEPs) were also determined. The in vitro antimicrobial activities of I against three Gram-positive bacteria, three Gram-negative bacteria and two fungi were determined. The compound was compared with several control drugs and showed better activity than the amoxicillin standard against Gram-positive bacteria B. subtilis, S. aureus and E. faecalis, and Gramnegative bacteria E. coli, K. pneumoniae and P. aeruginosa. The density functional theory (DFT)-optimized structure of the small molecule was used to perform molecular docking studies with proteins from experimentally studied bacterial and fungal organisms using AutoDock to determine the most preferred binding mode of the ligand within the protein cavity. A druglikeness assay and ADME (absorption, distribution, metabolism and excretion) and toxicology studies were carried out and predict a good drug-like character.
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    Detection of Bacterial Diversity of Various Habitats in Çorum Province and Its Criminalistics Contribution to Possible Crime Scene Studies
    (2024) Balci, Esra; Tatar, Demet; Veyisoglu, Aysel; Tokatlı, Ali
    Microorganisms are not homogeneously distributed in environments, soil systems are heterogeneous. Soil can be an important evidence value in forensic investigations. It is among the important evidences that contribute to the solution of forensic events in forensic sciences. Bacteria contained in the soil are microbiological evidences. Not all bacteria can be cultured by conventional methods and the amount of cultured bacteria remains limited. Metagenomic studies have been carried out for non-culturable Bacteria. The aim of this study is to perform DNA isolation from soil samples taken from Yeşil Lake (swamp), Faculty of Arts and Sciences garden, agricultural land, Sıklık (forest area) regions of Çorum Province in Türkiye and to determine bacterial diversity by metagenomic analysis of DNA isolated from soil samples. Density and differences of isolates according to habitats were determined. It is thought that the result of this study can shed light on previous crime scene studies in the determined habitats and will contribute to possible future crime scene studies and forensic science that may occur later.
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    Determination of Amylolytic and Proteolytic Enzyme Activity Characteristics of Thermophilic Actinobacteria Isolated from Amasya- Terziköy Thermal Spring
    (2025) Tatar, Demet; Duyar, Hünkar Avni; Veyisoglu, Aysel; Yılmaz, Adem Barış
    Actinobacteria represent a group of Gram-positive microorganisms characterized by high G+C content and are commonly found in both terrestrial and aquatic ecosystems. These bacteria are notable for their capacity to synthesize a wide range of bioactive compounds, including antibiotics and industrial enzymes. Their ability to withstand extreme environmental conditions allows them to colonize challenging habitats, such as thermal springs. These geothermal environments are critical not only for harboring microbial diversity but also for the discovery of novel bioactive substances with therapeutic potential. This study focused on isolating thermophilic actinobacteria from Terziköy Thermal Spring in Amasya, Türkiye, followed by phylogenetic analysis through 16S rRNA gene sequencing. The enzymatic capacities of the isolates—specifically amylase and protease production—were also assessed. Fourteen of the 21 isolates underwent 16S rRNA sequencing, resulting in the identification of eight Streptomyces, four Nocardia, one Actinomadura, and one Pseudonocardia species. Among these, seven isolates demonstrated amylase activity, and one displayed protease production. Notably, one Streptomyces isolate exhibited both enzymatic activities. These results highlight the phylogenetic diversity of thermophilic actinobacteria in geothermal environments and their potential as sources of thermostable enzymes for industrial applications.
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    Determination of the Antimicrobial Potential of Naturally Grown Rosehip (Rosa canina L.) Fruit in the Giresun Region
    (2025) Tatar, Demet; Veyisoglu, Aysel
    The rise of antibiotic-resistant microorganisms has become a major global public health challenge, prompting the search for alternative antimicrobial agents. In this study, the antimicrobial activity of Rosa canina (rosehip) fruit extract, collected from the Alucra Plateau in Giresun, Türkiye, was evaluated under in vitro conditions. Methanolic extracts were tested against Gram-positive (Bacillus subtilis, Staphylococcus aureus) and Gram-negative (Escherichia coli, Enterobacter aerogenes) bacteria, as well as fungal strains (Candida albicans, Candida glabrata, Candida tropicalis) using the disk diffusion method. At a concentration of 30 mg/mL, the extract demonstrated significant inhibition zones against both bacterial and fungal pathogens. The most notable antifungal effects were observed against C. glabrata (20 mm) and C. tropicalis (16 mm). The absence of antimicrobial activity in the 25% DMSO control confirmed that the effects were solely attributable to the plant extract. These findings suggest that R. canina possesses promising natural antimicrobial properties and could serve as a complementary treatment option, particularly against multidrug-resistant pathogens.
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    Diversity and antimicrobial activity of culturable actinobacteria isolated from the sediment of Sarikum Lake
    (Taylor & Francis Ltd, 2021) Veyisoglu, Aysel; Tatar, Demet
    In this study, actinobacteria isolated from Sarikum Lake sediment were characterized (Sinop-Turkey). A total of 48 actinobacteria were isolated using ten selective media with the dilution-plating method. The phylogenetic analysis according to 16S rRNA gene sequencing showed that 40% of the isolated strains belong to the widely distributed genus of Streptomyces, 36% belong to genus of Micromonospora, 24% of the isolates belong to rare genera such as Rhodococcus, Plantactinospora, Nonomuraea, Actinomadura and Streptosporangium. Most of the isolated strains belong to the genus Streptomyces (40%) and two isolates may be new species. All of the isolates were tested for antimicrobial activity; only 12 isolates exhibited antimicrobial activity. Nevertheless, 11 isolates were active against gram-positive, 5 were potential against gram-negative and no isolates had any effect against pathogenic fungi. All of the 48 isolates were analysed for genes encoding nonribosomal peptide synthetases (NRPS) and polyketide synthases (PKS). In all of the 48 isolates were detected NRPS sequences, PKS-I in 28 isolates and PKS-II in 22 isolates. PKS-I - PKS-II - NRPS genes were identified in 17 isolates.
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    Enhanced kinetic performance and stability of catalase immobilized on epoxy-functionalized kaolinite
    (Nature Portfolio, 2026) Erol, Kadir; Veyisoglu, Aysel; Tatar, Demet; Kocabas, Buket Bulut; Alacabey, Ihsan; Gokmese, Ebru
    The immobilization of catalase onto stable, reusable supports is crucial for efficient peroxide-based biocatalytic applications. In this study, catalase was immobilized for the first time onto epoxy-functionalized kaolinite particles prepared via surface silanization with (3-glycidyloxypropyl)trimethoxysilane. Structural and surface characterizations confirmed successful organosilane grafting while preserving the layered kaolinite framework. The modified support exhibited rapid enzyme uptake and a high immobilization capacity of approximately 300 mg g(-1). Kinetic analysis showed a substantial decrease in K-m from 57.3 mM (free catalase) to 21.6 mM after immobilization, indicating enhanced substrate affinity. In contrast, V-max decreased due to diffusion limitations typical of heterogeneous systems. Despite this, catalytic efficiency increased nearly 1.8-fold. Moreover, immobilized catalase demonstrated significantly improved operational reusability and long-term storage stability compared to the free enzyme. These results highlight silanized kaolinite as a robust, low-cost, and efficient mineral-based support for catalase immobilization, with strong potential for environmental and industrial biocatalytic applications.
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    Experimental and theoretical investigations on a furan-2-carboxamide-bearing thiazole: synthesis, molecular characterization by IR/NMR/XRD, electronic characterization by DFT, Hirshfeld surface analysis and biological activity
    (Int Union Crystallography, 2022) Cakmak, Sukriye; Kirca, Basak Kosar; Veyisoglu, Aysel; Yakan, Hasan; Ersanli, Cem Cuneyt; Kutuk, Halil
    A thiazole-based heterocyclic amide, namely, N-(thiazol-2-yl)furan-2-carbox-amide, C8H6N2O2S, was synthesized and investigated for its antimicrobial activity. The structure was characterized by elemental analysis and IR, H-1 NMR, and C-13 NMR spectroscopy. The molecular and electronic structures were investigated experimentally by single-crystal X-ray diffraction (XRD) and theoretically by density functional theory (DFT) modelling. The compound crystallized in the monoclinic space group P2(1)/n and the asymmetric unit contains two symmetrically independent molecules. Several noncovalent interactions were recorded by XRD and analysed with Hirshfeld surface analysis (HSA) calculations. Natural bond orbital, molecular electrostatic potential, second-order nonlinear optical and thermodynamic property analyses were also carried out using the DFT/B3LYP method. The title compound was evaluated for antimicrobial activity against eight microorganisms consisting of Gram-negative bacteria, Gram-positive bacteria and fungi. The compound showed good antimicrobial activity against the eight tested microorganisms. This suggests that the compound merits further study for potential pharmacological and medical applications.
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    Genomic Analysis of a Novel Heavy Metal Resistant Isolate from a Black Sea Contaminated Sediment with the Potential to Degrade Alkanes: Plantactinospora alkalitolerans sp. nov.
    (Mdpi, 2022) Carro, Lorena; Veyisoglu, Aysel; Guven, Kiymet; Schumann, Peter; Klenk, Hans-Peter; Sahin, Nevzat
    Microorganisms that grow in poorly studied environments are of special interest when new biotechnological applications are searched. The Melet river offshore sediments at the Black Sea have been described to contain an important number of contaminants from upstream industries which have been accumulating for years. Bacteria of such habitats must be adapted to the presence of those compounds and in some cases, are able to use them as carbon sources. In the analysis of some samples recovered from this environment, an actinobacterial strain was isolated, named as S1510(T), and its taxonomic position was determined using a combination of phenotypic and genotypic properties. Strain S1510(T) presented phenotypic properties typical of members of the family Micromonosporaceae and was assigned to the Plantactinospora genus, based on the phylogenetic analyses of the 16S rRNA gene and whole-genome sequences. Low dDDH (digital DNA-DNA hybridization) values with other members of the genus confirmed that Plantactinospora sp. S1510(T) represents a novel species, and is proposed with the new name Plantactinospora alkalitolerans. The strain presented characteristics not previously described for other species in the genus, such as its high tolerance to alkaline pHs, the presence of genes related to the production and degradation of alkanes (oleABCD, ssuAD, almA), the degradation of several aromatic compounds, and the tolerance to high heavy metal concentrations. In addition, Plantactinospora sp. S1510(T) presents several bioclusters to produce nonribosomal peptide-synthetases, terpenes, polyketide synthases, and bacteriocins, that possess low similarities with known compounds.
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    Heterocyclic amides derived from 2-thiopheneacetic acid: Synthesis, characterization and antimicrobial activity evaluation
    (TUBITAK, 2021) Cakmak, Sukriye; Veyisoglu, Aysel
    Synthesized the heterocyclic amide derivatives (I-IV) were from 2-thiophene acetic acid in two steps. In the first step, the intermediate acylation agent formed and isolated, then subjected to aminolysis and obtained the corresponding amide derivatives. The structures of compounds obtained were characterized by FT-IR,13C NMR,1H NMR, spectroscopies and elemental analysis techniques. The antimicrobial activities of these four compounds against Gram-negative bacteria, Gram-positive bacteria and fungi were investigated using the minimum inhibition concentration method. As a result, compounds (I and III) exhibited better good antibacterial activities against Staphylococcus aureus, Enterococcus faecalis and Pseudomonas aeruginosa compared to the commercially standard antibacterial agent of amoxicillin. © 2021, TUBITAK. All rights reserved.
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    Investigation of the antimicrobial activity of water and methanol extracts of Salvadora persica L. (Miswak) plant against some pathogenic microorganisms
    (Gültekin ÖZDEMIR, 2024) Veyisoglu, Aysel; Balta, Yusuf Emre; Tatar, Demet
    The toothbrush tree Salvadora persica L, also called miswak, belonging to the family Salvadoraceae, is one of the most important of the 182 plant species used as chewing sticks. It is widely used in many Asian, African and Middle Eastern countries. The roots, branches and stems of this plant have been used for oral hygiene and small miswak sticks have been used as toothpicks for oral hygiene. In this study, commercially purchased Salvadora persica, L. (Miswak) plant used in oral hygiene were tested against seven pathogenic bacteria (Bacillus cereus ATCC 10987, Bacillus subtilis ATCC 6623, Staphylococcus aureus ATCC 25923, Enterococcus faecalis ATCC 29212, Escherichia coli ATCC 25922, Klebsiella pneumoniae ATCC 70060, Pseudomonas aeruginosa ATCC 27853) and two fungi (Candida albicans ATCC 10231 and Aspergillus niger ATCC 16404) at eight different concentrations (200 mg/ml, 100 mg/ml, 50 mg/ml, 25 mg/ml, 12. 5 mg/ml, 6 mg/ml, 3 mg/ml and 1 mg/ml) were determined. While the aqueous extract did not show any antimicrobial activity against seven pathogens, the methanol extract showed activity against three pathogens. The methanol extract of S. persica showed antimicrobial activity against Bacillus cereus ATCC 10987, Bacillus subtilis ATCC 6623 and Klebsiella pneumoniae ATCC 70060 strains.
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    Investigation of the Antimicrobial Effects of Traditionally Prepared Vinegars from Plantago major L. and Hypericum perforatum L. Collected in Ordu Province on Selected Pathogenic Microorganisms
    (2025) Tatar, Demet; Veyisoglu, Aysel; Tepeköy, İrem
    In this study, the antimicrobial effects of traditionally fermented vinegars produced from Plantago major L. (common plantain) and Hypericum perforatum L. (St. John’s Wort), collected from the Ünye district of Ordu Province, Türkiye, were evaluated. The vinegar samples were tested against 11 pathogenic microorganisms, including Gram-positive and Gram-negative bacteria as well as Candida species, using the agar disk diffusion method. The results showed that both types of vinegar exhibited significant antimicrobial activity, particularly at undiluted (1:1) and 1/2 dilutions. The highest antibacterial effect for P. major vinegar was observed against Proteus vulgaris (15.33±0.33 mm). Antifungal activity, however, was generally lower compared to bacterial inhibition. The pH values of P. major and H. perforatum vinegars were measured as 2.62 and 2.72, respectively, indicating strong acidity, which likely contributes to their antimicrobial properties. These findings suggest that vinegars produced from medicinal plants through traditional fermentation methods have the potential to serve as natural antimicrobial agents. Further biochemical studies are recommended to explore their potential applications in pharmaceutical and food safety fields.
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    Isolation, Molecular Characterization and Determination of Antagonistic Properties of Alkalitolerant Streptomyces Members from Van Lake-Çarpanak Island Soil
    (2024) Veyisoglu, Aysel; Tatar, Demet; Duyar, Hünkar Avni; Tokatlı, Ali; Tokatlı, Ali
    Çarpanak Island is one of the islands in Lake Van, the easternmost and largest lake in Türkiye. In this study, soil samples taken from three different locations of Çarpanak island, Lake Van, were inoculated into 4 different selective isolation media using the dilution plate technique, and 42 actinomycete isolates were stocked. PCR amplification of the 16S rRNA gene of 13 isolates selected according to their morphological differences was performed with 27F and 1525R primers. Phylogenetic trees were constructed with the neighbor-joining algorithm in the MEGA 7.0 software. According to 16S rRNA gene sequence analysis, it was determined that 13 isolates belonged to the genus Streptomyces. The antimicrobial activities of 13 isolates against 8 pathogens and the ability of these isolates to produce lipase, amylase, protease and pectinase were determined. Except for CA43 and CA62 isolates, other Streptomyces isolates have the ability to produce lipase. Other Streptomyces isolates except CA59 and CA94 can synthesize amylase. In addition, 3 of the 13 Streptomyces isolates, CA40, CA61 and CA94, do not have the ability to synthesize protease. No isolate is capable of producing pectinase enzyme. As a result of this study, it was observed that most of the Streptomyces isolates had higher lipase enzyme production abilities than other enzyme groups.
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    Kargı ve Osmancık Çevresindeki Yayla Topraklarının Aktinomiset Çeşitliliği
    (2020) Tatar, Demet; Veyisoglu, Aysel
    Bu çalışmada Çorum iline bağlı Kargı (Eğinönü), Abdullah ve Osmancık-Başpınar Karaca yaylalarından alınan toprak örneklerinden izole edilen aktinomiset suşlarının 16S rRNA gen bölgesi analizlerine göre aktinomiset çeşitliliğinin saptanması amaçlandı. Dilüsyon plak tekniğinin uygulandığı izolasyon çalışmasında 5 farklı seçici besiyeri kullanıldı. İzolatların beş farklı kültür ortamında kültürel karakterizasyonları yapıldı. Test izolatlarının 16S rRNA gen bölgesinin PZR amplifikasyonu 27F ve 1525R primerleri ile gerçekleştirildi. Filogenetik ağaçlar,MEGA 7.0 yazılım programında neighbor-joining algoritmasıyla oluşturuldu. 5 farklı izolasyon besiyerinden toplam 15 mikroorganizma seçildi ve 16S rRNA gen dizi analizine göre 15 izolatın, altısı Nocardia cinsi üyesi, beşi Streptomyces cinsi üyesi, biri Haloactinopolyspora cinsi üyesi, biri Micromonospora cinsi üyesi, biriNocardiopsis cinsi üyesi ve birinin de Rhodococcus cinsi üyesi olduğu tespit edildi.
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    Micromonospora orduensis sp. nov., isolated from deep marine sediment
    (Springer, 2020) Veyisoglu, Aysel; Carro, Lorena; Cetin, Demet; Igual, Jose M.; Klenk, Hans-Peter; Sahin, Nevzat
    A novel actinobacterial strain, designated S2509(T), was isolated from marine sediment collected by a dredge at a depth of 45 m along Melet River offshore of the southern Black Sea coast, Ordu, Turkey. The cell wall peptidoglycan of strain was found to contain meso-diaminopimelic acid and 3-OH-diaminopimelic acid. The whole cell sugars detected were arabinose, glucose, rhamnose, ribose and xylose. The diagnostic phospholipids of strain S2509(T) were found to be diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, a glycolipid and two unidentified phospholipids. The predominant menaquinones were identified as MK-9(H-8), MK-9(H-6), MK-10(H-8), MK-9(H-4), MK-10(H-4) and MK-10(H-6). The major cellular fatty acids were found to be iso-C-16:0, iso-C-15:0 and 10-methyl C-17:0. The taxonomic position of the strain was established using a polyphasic approach, showing that S2509(T) strain belongs to the genus Micromonospora. Phylogenetic analysis based on the 16S rRNA gene sequence of strain S2509(T) showed that it is closely related to the type strain of Micromonospora chokoriensis DSM 45160(T) (99.37% sequence similarity), and phylogenetically clustered with Micromonospora inaquosa LB39(T) (99.37%), Micromonospora lupini Lupac 14N(T) (99.16%), Micromonospora violae NEAU-zh8(T) (99.23%) and Micromonospora taraxaci NEAU-P5(T) (99.03%). The phylogenetic analysis based on the gyrB gene sequence of strain S2509(T) confirmed its close relationship with M. chokoriensis JCM 13247(T) (96.5% sequence similarity). Whole genome sequences confirmed by digital DNA-DNA hybridization analysis that the strain S2509(T) represents a novel species in the genus Micromonospora, for which the name Micromonospora orduensis sp. nov. is proposed. The type strain is S2509(T) (=DSM 45926(T) = KCTC 29201(T)).
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    Micromonospora phytophila sp nov and Micromonospora luteiviridis sp nov., isolated as natural inhabitants of plant nodules
    (Microbiology Soc, 2018) Carro, Lorena; Veyisoglu, Aysel; Riesco, Raul; Sproeer, Cathrin; Klenk, Hans-Peter; Sahin, Nevzat; Trujillo, Martha E.
    Two actinobacterial isolates, strains SG15(T) and SGB14(T), were recovered through a microbial diversity study of nitrogen fixing nodules from Pisum sativum plants collected in Salamanca (Spain). The taxonomic status of these isolates was determined using a polyphasic approach and both presented chemotaxonomic and morphological properties consistent with their classification in the genus Micromonospora. For strains SG15(T) and SGB14(T), the highest 16S rRNA gene sequence similarities were observed with Micromonospora coxensis JCM 13248(T) (99.2 %) and Micromonospora purpureochromogenes DSM 43821(T) (99.4 %), respectively. However, strains SG15(T) and SGB14(T) were readily distinguished from their phylogenetic neighbours both genetically and phenotypically indicating that they represent two new Micromonospora species. The following names are proposed for these species: Micromonospora phytophila sp. nov. type strain SG15(T) (=CECT 9369(T); =DSM 105363(T)), and Micromonospora luteiviridis sp. nov. type strain SGB14(T) (=CECT 9370(T); =DSM 105362(T)).
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    Micromonospora profundi sp nov., isolated from deep marine sediment
    (Microbiology Soc, 2016) Veyisoglu, Aysel; Carro, Lorena; Cetin, Demet; Guven, Kiymet; Sproeer, Cathrin; Poetter, Gabriele; Klenk, Hans-Peter
    A novel actinobacterial strain, designated DS3010(T), was isolated from a Black Sea marine sediment and characterized using a polyphasic approach. The strain was shown to have chemotaxonomic, morphological and phylogenetic properties consistent with classification as representing a member of the genus Micromonospora. Comparative 16S rRNA gene sequence studies showed that the strain was most closely related to the type strains of Micromonospora saelicesensis (99.5 %), Micromonospora chokoriensis (99.4 %) and Micromonospora violae (99.3 %). Similarly, a corresponding analysis based on partial gyrB gene sequences showed that it formed a distinct phyletic branch in a subclade that included the type strains of Micromonosporazamorensis, 'Micromonospora zeae', 'Micromonospora jinlongensis', M. saelicesensis and Micromonospora lupini. DS3010(T) was distinguished from its closest phylogenetic neighbours by low levels of DNA-DNA relatedness and by a combination of chemotaxonomic and phenotypic properties. On the basis of these data, it is proposed that the isolate should be assigned to the genus Micromonospora as Micromonospora profundi sp. nov. with isolate DS3010(T) (=DSM 45981(T) =KCTC 29243(T)) as the type strain.