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Öğe A study of three bacteria isolated from marine sediment and description of Micromonospora globispora sp. nov.(Elsevier Gmbh, 2019) Carro, Lorena; Veyisoglu, Aysel; Cetin, Demet; Igual, Jose M.; Klenk, Hans-Peter; Trujillo, Martha E.; Sahin, NevzatDuring a study looking for the isolation of new actinobacteria strains with potential for antibiotic production from deep marine sediment, three strains were collected with a morphology similar to the one described for the Micromonospora genus. A polyphasic study was designed to determine the taxonomic affiliation of the strains S2901(T), S2903, and S2904. All the strains showed chemotaxonomic properties in line with their classification in the genus Micromonospora, meso-diaminopimelic acid in the wall peptidoglycan, a tetrahydrogenated menaquinone with nine isoprene units as major respiratory quinone, iso-C-15:0 and iso-C-16:0 as major fatty acids and diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol as major polar lipids. The 16S rRNA gene sequences of strain S2901(T), S2903, and S2904 showed the highest similarity (99.2%) with the type strain of Micromonospora halophytica DSM 43171(T), forming an independent branch in the phylogenetic gene tree. Their independent position was confirmed with gyrB gene and MLSA phylogenies. Whole genome sequences confirmed by digital DNA-DNA hybridization analysis that the isolates should be assigned to a new species within the genus Micromonospora for which the name Micromonospora globispora sp. nov. (S2901(T), S2903 and S2904) is proposed. (C) 2018 Elsevier GmbH. All rights reserved.Öğe Antimicrobial magnetic poly(GMA) microparticles: synthesis, characterization and lysozyme immobilization(Walter De Gruyter Gmbh, 2021) Erol, Kadir; Tatar, Demet; Veyisoglu, Aysel; Tokatli, AliMicron-sized magnetic particles currently find a wide range of applications in many areas including biotechnology, biochemistry, colloid sciences and medicine. In this study, magnetic poly(glycidyl methacrylate) microparticles were synthesized by providing a polymerization around Fe(II)-Ni(II) magnetic double salt. Adsorption of lysozyme protein from aqueous systems was studied with these particles. Adsorption studies were performed with changing pH values, variable amount of adsorbent, different interaction times and lysozyme amounts. The adsorption capacity of the particles was investigated, and a value of about 95.6 mg lysozyme/g microparticle was obtained. The enzyme activity of the immobilized lysozyme was examined and found to be more stable and reusable compared to the free enzyme. The immobilized enzyme still showed 80% activity after five runs and managed to maintain 78% of its initial activity at the end of 60 days. Besides, in the antimicrobial analysis study for six different microorganisms, the minimum inhibitory concentration value of lysozyme immobilized particles was calculated as 125 tg/mL like free lysozyme. Finally, the adsorption interaction was found to be compatible with the Langmuir isotherm model. Accordingly, it can be said that magnetic poly(GMA) microparticles are suitable materials for lysozyme immobilization and immobilized lysozyme can be used in biotechnological studies.Öğe Design, synthesis and spectroscopic and structural characterization of novel N-(2-hydroxy-5-methyl-phenyl)-2,3-dimethoxybenzamide: DFT, Hirshfeld surface analysis, antimicrobial activity, molecular docking and toxicology(Int Union Crystallography, 2022) Cakmak, Sukriye; Aycan, Tugba; Ozturk, Filiz; Veyisoglu, AyselThe novel compound N-(2-hydroxy-5-methylphenyl)-2,3-dimethoxybenzamide, C16H17NO4, I, was prepared by a two-step reaction and then characterized by elemental analysis and X-ray diffraction (XRD) methods. Moreover, its spectroscopic properties were investigated by FT-IR and H-1 and C-13 NMR. Compound I crystallized in the monoclinic space group P2(1)/c and the molecular geometry is not planar, being divided into three planar regions. Supramolecular structures are formed by connecting units via hydrogen bonds. The ground-state molecular structure of I was optimized by the DFT-B3LYP/6-31G(d,p) method and the theoretical structure was compared with that obtained by X-ray diffraction. Intermolecular interactions in the crystal network were studied by two-dimensional (2D) and three-dimensional (3D) Hirshfeld analyses. The calculated electronic transition results were examined and the molecular electrostatic potentials (MEPs) were also determined. The in vitro antimicrobial activities of I against three Gram-positive bacteria, three Gram-negative bacteria and two fungi were determined. The compound was compared with several control drugs and showed better activity than the amoxicillin standard against Gram-positive bacteria B. subtilis, S. aureus and E. faecalis, and Gramnegative bacteria E. coli, K. pneumoniae and P. aeruginosa. The density functional theory (DFT)-optimized structure of the small molecule was used to perform molecular docking studies with proteins from experimentally studied bacterial and fungal organisms using AutoDock to determine the most preferred binding mode of the ligand within the protein cavity. A druglikeness assay and ADME (absorption, distribution, metabolism and excretion) and toxicology studies were carried out and predict a good drug-like character.Öğe Detection of Bacterial Diversity of Various Habitats in Çorum Province and Its Criminalistics Contribution to Possible Crime Scene Studies(2024) Balci, Esra; Tatar, Demet; Veyisoglu, Aysel; Tokatlı, AliMicroorganisms are not homogeneously distributed in environments, soil systems are heterogeneous. Soil can be an important evidence value in forensic investigations. It is among the important evidences that contribute to the solution of forensic events in forensic sciences. Bacteria contained in the soil are microbiological evidences. Not all bacteria can be cultured by conventional methods and the amount of cultured bacteria remains limited. Metagenomic studies have been carried out for non-culturable Bacteria. The aim of this study is to perform DNA isolation from soil samples taken from Yeşil Lake (swamp), Faculty of Arts and Sciences garden, agricultural land, Sıklık (forest area) regions of Çorum Province in Türkiye and to determine bacterial diversity by metagenomic analysis of DNA isolated from soil samples. Density and differences of isolates according to habitats were determined. It is thought that the result of this study can shed light on previous crime scene studies in the determined habitats and will contribute to possible future crime scene studies and forensic science that may occur later.Öğe Diversity and antimicrobial activity of culturable actinobacteria isolated from the sediment of Sarikum Lake(Taylor & Francis Ltd, 2021) Veyisoglu, Aysel; Tatar, DemetIn this study, actinobacteria isolated from Sarikum Lake sediment were characterized (Sinop-Turkey). A total of 48 actinobacteria were isolated using ten selective media with the dilution-plating method. The phylogenetic analysis according to 16S rRNA gene sequencing showed that 40% of the isolated strains belong to the widely distributed genus of Streptomyces, 36% belong to genus of Micromonospora, 24% of the isolates belong to rare genera such as Rhodococcus, Plantactinospora, Nonomuraea, Actinomadura and Streptosporangium. Most of the isolated strains belong to the genus Streptomyces (40%) and two isolates may be new species. All of the isolates were tested for antimicrobial activity; only 12 isolates exhibited antimicrobial activity. Nevertheless, 11 isolates were active against gram-positive, 5 were potential against gram-negative and no isolates had any effect against pathogenic fungi. All of the 48 isolates were analysed for genes encoding nonribosomal peptide synthetases (NRPS) and polyketide synthases (PKS). In all of the 48 isolates were detected NRPS sequences, PKS-I in 28 isolates and PKS-II in 22 isolates. PKS-I - PKS-II - NRPS genes were identified in 17 isolates.Öğe Experimental and theoretical investigations on a furan-2-carboxamide-bearing thiazole: synthesis, molecular characterization by IR/NMR/XRD, electronic characterization by DFT, Hirshfeld surface analysis and biological activity(Int Union Crystallography, 2022) Cakmak, Sukriye; Kirca, Basak Kosar; Veyisoglu, Aysel; Yakan, Hasan; Ersanli, Cem Cuneyt; Kutuk, HalilA thiazole-based heterocyclic amide, namely, N-(thiazol-2-yl)furan-2-carbox-amide, C8H6N2O2S, was synthesized and investigated for its antimicrobial activity. The structure was characterized by elemental analysis and IR, H-1 NMR, and C-13 NMR spectroscopy. The molecular and electronic structures were investigated experimentally by single-crystal X-ray diffraction (XRD) and theoretically by density functional theory (DFT) modelling. The compound crystallized in the monoclinic space group P2(1)/n and the asymmetric unit contains two symmetrically independent molecules. Several noncovalent interactions were recorded by XRD and analysed with Hirshfeld surface analysis (HSA) calculations. Natural bond orbital, molecular electrostatic potential, second-order nonlinear optical and thermodynamic property analyses were also carried out using the DFT/B3LYP method. The title compound was evaluated for antimicrobial activity against eight microorganisms consisting of Gram-negative bacteria, Gram-positive bacteria and fungi. The compound showed good antimicrobial activity against the eight tested microorganisms. This suggests that the compound merits further study for potential pharmacological and medical applications.Öğe Genomic Analysis of a Novel Heavy Metal Resistant Isolate from a Black Sea Contaminated Sediment with the Potential to Degrade Alkanes: Plantactinospora alkalitolerans sp. nov.(Mdpi, 2022) Carro, Lorena; Veyisoglu, Aysel; Guven, Kiymet; Schumann, Peter; Klenk, Hans-Peter; Sahin, NevzatMicroorganisms that grow in poorly studied environments are of special interest when new biotechnological applications are searched. The Melet river offshore sediments at the Black Sea have been described to contain an important number of contaminants from upstream industries which have been accumulating for years. Bacteria of such habitats must be adapted to the presence of those compounds and in some cases, are able to use them as carbon sources. In the analysis of some samples recovered from this environment, an actinobacterial strain was isolated, named as S1510(T), and its taxonomic position was determined using a combination of phenotypic and genotypic properties. Strain S1510(T) presented phenotypic properties typical of members of the family Micromonosporaceae and was assigned to the Plantactinospora genus, based on the phylogenetic analyses of the 16S rRNA gene and whole-genome sequences. Low dDDH (digital DNA-DNA hybridization) values with other members of the genus confirmed that Plantactinospora sp. S1510(T) represents a novel species, and is proposed with the new name Plantactinospora alkalitolerans. The strain presented characteristics not previously described for other species in the genus, such as its high tolerance to alkaline pHs, the presence of genes related to the production and degradation of alkanes (oleABCD, ssuAD, almA), the degradation of several aromatic compounds, and the tolerance to high heavy metal concentrations. In addition, Plantactinospora sp. S1510(T) presents several bioclusters to produce nonribosomal peptide-synthetases, terpenes, polyketide synthases, and bacteriocins, that possess low similarities with known compounds.Öğe Heterocyclic amides derived from 2-thiopheneacetic acid: Synthesis, characterization and antimicrobial activity evaluation(TUBITAK, 2021) Cakmak, Sukriye; Veyisoglu, AyselSynthesized the heterocyclic amide derivatives (I-IV) were from 2-thiophene acetic acid in two steps. In the first step, the intermediate acylation agent formed and isolated, then subjected to aminolysis and obtained the corresponding amide derivatives. The structures of compounds obtained were characterized by FT-IR,13C NMR,1H NMR, spectroscopies and elemental analysis techniques. The antimicrobial activities of these four compounds against Gram-negative bacteria, Gram-positive bacteria and fungi were investigated using the minimum inhibition concentration method. As a result, compounds (I and III) exhibited better good antibacterial activities against Staphylococcus aureus, Enterococcus faecalis and Pseudomonas aeruginosa compared to the commercially standard antibacterial agent of amoxicillin. © 2021, TUBITAK. All rights reserved.Öğe Investigation of the antimicrobial activity of water and methanol extracts of Salvadora persica L. (Miswak) plant against some pathogenic microorganisms(Gültekin ÖZDEMIR, 2024) Veyisoglu, Aysel; Balta, Yusuf Emre; Tatar, DemetThe toothbrush tree Salvadora persica L, also called miswak, belonging to the family Salvadoraceae, is one of the most important of the 182 plant species used as chewing sticks. It is widely used in many Asian, African and Middle Eastern countries. The roots, branches and stems of this plant have been used for oral hygiene and small miswak sticks have been used as toothpicks for oral hygiene. In this study, commercially purchased Salvadora persica, L. (Miswak) plant used in oral hygiene were tested against seven pathogenic bacteria (Bacillus cereus ATCC 10987, Bacillus subtilis ATCC 6623, Staphylococcus aureus ATCC 25923, Enterococcus faecalis ATCC 29212, Escherichia coli ATCC 25922, Klebsiella pneumoniae ATCC 70060, Pseudomonas aeruginosa ATCC 27853) and two fungi (Candida albicans ATCC 10231 and Aspergillus niger ATCC 16404) at eight different concentrations (200 mg/ml, 100 mg/ml, 50 mg/ml, 25 mg/ml, 12. 5 mg/ml, 6 mg/ml, 3 mg/ml and 1 mg/ml) were determined. While the aqueous extract did not show any antimicrobial activity against seven pathogens, the methanol extract showed activity against three pathogens. The methanol extract of S. persica showed antimicrobial activity against Bacillus cereus ATCC 10987, Bacillus subtilis ATCC 6623 and Klebsiella pneumoniae ATCC 70060 strains.Öğe Isolation, Molecular Characterization and Determination of Antagonistic Properties of Alkalitolerant Streptomyces Members from Van Lake-Çarpanak Island Soil(2024) Veyisoglu, Aysel; Tatar, Demet; Duyar, Hünkar Avni; Tokatlı, Ali; Tokatlı, AliÇarpanak Island is one of the islands in Lake Van, the easternmost and largest lake in Türkiye. In this study, soil samples taken from three different locations of Çarpanak island, Lake Van, were inoculated into 4 different selective isolation media using the dilution plate technique, and 42 actinomycete isolates were stocked. PCR amplification of the 16S rRNA gene of 13 isolates selected according to their morphological differences was performed with 27F and 1525R primers. Phylogenetic trees were constructed with the neighbor-joining algorithm in the MEGA 7.0 software. According to 16S rRNA gene sequence analysis, it was determined that 13 isolates belonged to the genus Streptomyces. The antimicrobial activities of 13 isolates against 8 pathogens and the ability of these isolates to produce lipase, amylase, protease and pectinase were determined. Except for CA43 and CA62 isolates, other Streptomyces isolates have the ability to produce lipase. Other Streptomyces isolates except CA59 and CA94 can synthesize amylase. In addition, 3 of the 13 Streptomyces isolates, CA40, CA61 and CA94, do not have the ability to synthesize protease. No isolate is capable of producing pectinase enzyme. As a result of this study, it was observed that most of the Streptomyces isolates had higher lipase enzyme production abilities than other enzyme groups.Öğe Kargi ve Osmancik Çevresindeki Yayla Topraklarinin Aktinomiset Çesitliligi(Bilecik Seyh Edebali Üniversitesi, 2020) Tatar, Demet; Veyisoglu, AyselBu çalismada Çorum iline bagli Kargi (Eginönü), Abdullah ve Osmancik-Baspinar Karaca yaylalarindan alinan toprak örneklerinden izole edilen aktinomiset suslarinin 16S rRNA gen bölgesi analizlerine göre aktinomiset çesitliliginin saptanmasi amaçlandi. Dilüsyon plak tekniginin uygulandigi izolasyon çalismasinda 5 farkli seçici besiyeri kullanildi. Izolatlarin bes farkli kültür ortaminda kültürel karakterizasyonlari yapildi. Test izolatlarinin 16S rRNA gen bölgesinin PZR amplifikasyonu 27F ve 1525R primerleri ile gerçeklestirildi. Filogenetik agaçlar, MEGA 7.0 yazilim programinda neighbor-joining algoritmasiyla olusturuldu. 5 farkli izolasyon besiyerinden toplam 15 mikroorganizma seçildi ve 16S rRNA gen dizi analizine göre 15 izolatin, altisi Nocardia cinsi üyesi, besi Streptomyces cinsi üyesi, biri Haloactinopolyspora cinsi üyesi, biri Micromonospora cinsi üyesi, biri Nocardiopsis cinsi üyesi ve birinin de Rhodococcus cinsi üyesi oldugu tespit edildi.Öğe Kargı ve Osmancık Çevresindeki Yayla Topraklarının Aktinomiset Çeşitliliği(2020) Tatar, Demet; Veyisoglu, AyselBu çalışmada Çorum iline bağlı Kargı (Eğinönü), Abdullah ve Osmancık-Başpınar Karaca yaylalarından alınan toprak örneklerinden izole edilen aktinomiset suşlarının 16S rRNA gen bölgesi analizlerine göre aktinomiset çeşitliliğinin saptanması amaçlandı. Dilüsyon plak tekniğinin uygulandığı izolasyon çalışmasında 5 farklı seçici besiyeri kullanıldı. İzolatların beş farklı kültür ortamında kültürel karakterizasyonları yapıldı. Test izolatlarının 16S rRNA gen bölgesinin PZR amplifikasyonu 27F ve 1525R primerleri ile gerçekleştirildi. Filogenetik ağaçlar,MEGA 7.0 yazılım programında neighbor-joining algoritmasıyla oluşturuldu. 5 farklı izolasyon besiyerinden toplam 15 mikroorganizma seçildi ve 16S rRNA gen dizi analizine göre 15 izolatın, altısı Nocardia cinsi üyesi, beşi Streptomyces cinsi üyesi, biri Haloactinopolyspora cinsi üyesi, biri Micromonospora cinsi üyesi, biriNocardiopsis cinsi üyesi ve birinin de Rhodococcus cinsi üyesi olduğu tespit edildi.Öğe Micromonospora orduensis sp. nov., isolated from deep marine sediment(Springer, 2020) Veyisoglu, Aysel; Carro, Lorena; Cetin, Demet; Igual, Jose M.; Klenk, Hans-Peter; Sahin, NevzatA novel actinobacterial strain, designated S2509(T), was isolated from marine sediment collected by a dredge at a depth of 45 m along Melet River offshore of the southern Black Sea coast, Ordu, Turkey. The cell wall peptidoglycan of strain was found to contain meso-diaminopimelic acid and 3-OH-diaminopimelic acid. The whole cell sugars detected were arabinose, glucose, rhamnose, ribose and xylose. The diagnostic phospholipids of strain S2509(T) were found to be diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, a glycolipid and two unidentified phospholipids. The predominant menaquinones were identified as MK-9(H-8), MK-9(H-6), MK-10(H-8), MK-9(H-4), MK-10(H-4) and MK-10(H-6). The major cellular fatty acids were found to be iso-C-16:0, iso-C-15:0 and 10-methyl C-17:0. The taxonomic position of the strain was established using a polyphasic approach, showing that S2509(T) strain belongs to the genus Micromonospora. Phylogenetic analysis based on the 16S rRNA gene sequence of strain S2509(T) showed that it is closely related to the type strain of Micromonospora chokoriensis DSM 45160(T) (99.37% sequence similarity), and phylogenetically clustered with Micromonospora inaquosa LB39(T) (99.37%), Micromonospora lupini Lupac 14N(T) (99.16%), Micromonospora violae NEAU-zh8(T) (99.23%) and Micromonospora taraxaci NEAU-P5(T) (99.03%). The phylogenetic analysis based on the gyrB gene sequence of strain S2509(T) confirmed its close relationship with M. chokoriensis JCM 13247(T) (96.5% sequence similarity). Whole genome sequences confirmed by digital DNA-DNA hybridization analysis that the strain S2509(T) represents a novel species in the genus Micromonospora, for which the name Micromonospora orduensis sp. nov. is proposed. The type strain is S2509(T) (=DSM 45926(T) = KCTC 29201(T)).Öğe Micromonospora phytophila sp nov and Micromonospora luteiviridis sp nov., isolated as natural inhabitants of plant nodules(Microbiology Soc, 2018) Carro, Lorena; Veyisoglu, Aysel; Riesco, Raul; Sproeer, Cathrin; Klenk, Hans-Peter; Sahin, Nevzat; Trujillo, Martha E.Two actinobacterial isolates, strains SG15(T) and SGB14(T), were recovered through a microbial diversity study of nitrogen fixing nodules from Pisum sativum plants collected in Salamanca (Spain). The taxonomic status of these isolates was determined using a polyphasic approach and both presented chemotaxonomic and morphological properties consistent with their classification in the genus Micromonospora. For strains SG15(T) and SGB14(T), the highest 16S rRNA gene sequence similarities were observed with Micromonospora coxensis JCM 13248(T) (99.2 %) and Micromonospora purpureochromogenes DSM 43821(T) (99.4 %), respectively. However, strains SG15(T) and SGB14(T) were readily distinguished from their phylogenetic neighbours both genetically and phenotypically indicating that they represent two new Micromonospora species. The following names are proposed for these species: Micromonospora phytophila sp. nov. type strain SG15(T) (=CECT 9369(T); =DSM 105363(T)), and Micromonospora luteiviridis sp. nov. type strain SGB14(T) (=CECT 9370(T); =DSM 105362(T)).Öğe Micromonospora profundi sp nov., isolated from deep marine sediment(Microbiology Soc, 2016) Veyisoglu, Aysel; Carro, Lorena; Cetin, Demet; Guven, Kiymet; Sproeer, Cathrin; Poetter, Gabriele; Klenk, Hans-PeterA novel actinobacterial strain, designated DS3010(T), was isolated from a Black Sea marine sediment and characterized using a polyphasic approach. The strain was shown to have chemotaxonomic, morphological and phylogenetic properties consistent with classification as representing a member of the genus Micromonospora. Comparative 16S rRNA gene sequence studies showed that the strain was most closely related to the type strains of Micromonospora saelicesensis (99.5 %), Micromonospora chokoriensis (99.4 %) and Micromonospora violae (99.3 %). Similarly, a corresponding analysis based on partial gyrB gene sequences showed that it formed a distinct phyletic branch in a subclade that included the type strains of Micromonosporazamorensis, 'Micromonospora zeae', 'Micromonospora jinlongensis', M. saelicesensis and Micromonospora lupini. DS3010(T) was distinguished from its closest phylogenetic neighbours by low levels of DNA-DNA relatedness and by a combination of chemotaxonomic and phenotypic properties. On the basis of these data, it is proposed that the isolate should be assigned to the genus Micromonospora as Micromonospora profundi sp. nov. with isolate DS3010(T) (=DSM 45981(T) =KCTC 29243(T)) as the type strain.Öğe Micromonospora yasonensis sp nov., isolated from a Black Sea sediment(Springer, 2016) Veyisoglu, Aysel; Carro, Lorena; Guven, Kiymet; Cetin, Demet; Sproeer, Cathrin; Schumann, Peter; Klenk, Hans-PeterA Micromonospora strain, designated DS3186(T), isolated from sediment collected from the Black Sea off the Yason Peninsula, Ordu, Turkey, was examinated using a polyphasic approach. The strain was found to have chemotaxonomic, morphological and phylogenetic properties consistent with its clasification in the genus Micromonospora. A comparative 16S rRNA gene sequence analysis showed that the strain was closely related to the type strains of Micromonospora olivasterospora (99.0 %), Micromonospora equina (98.8 %), Micromonospora rhizosphaerae (98.8 %) and Micromonospora viridifaciens (98.8 %); low levels of DNA-DNA relatednes were found between the isolate and the M. olivasterospora and M. rhizosphaerae strains. Corresponding phylogenetic analysis based on partial gyrB gene sequences showed that strain DS3186(T) formed a subclade with the type strains of Micromonospora eburnea, M. equina, Micromonospora narathiwatensis and M. viridifaciens. Strain DS3186(T) was distinguished from its close phylogenetic neighbours using a combination of chemotaxonomic, morphological and physiological properties. Consequently, it is proposed that strain DS3186(T) represents a novel Micromonospora species for which the name Micromonospora yasonensis sp. nov. is proposed. The type strain is DS3186(T) (=DSM 45980(T) = KCTC 29433(T)).Öğe Microvirga makkahensis sp nov., and Microvirga arabica sp nov., isolated from sandy arid soil(Springer, 2016) Veyisoglu, Aysel; Tatar, Demet; Saygin, Hayrettin; Inan, Kadriye; Cetin, Demet; Guven, Kiymet; Tuncer, MunirThe taxonomic positions of two Gram-negative strains, SV1470(T) and SV2184P(T), isolated from arid soil samples, were determined using a polyphasic approach. Analysis of the 16S rRNA gene and the concatenated sequences of three housekeeping gene loci (dnaK, rpoB and gyrB) confirmed that the strains belong to the genus Microvirga. Strain SV1470(T) was found to be closely related to Microvirga vignae BR3299(T) (98.8 %), Microvirga flocculans TFBT (98.3 %) and Microvirga lupini Lut6(T) (98.2 %), whilst similarity to other type strains of the genus ranged from 97.8 to 96.3 %; strain SV2184P(T) was found to be closely related to Microvirga aerilata 5420S-16(T) (98.0 %), Microvirga zambiensis WSM3693(T) (97.8 %) and M. flocculans ATCC BAA-817(T) (97.4 %), whilst similarity to other type strains of the genus ranged from 97.2 to 95.9 %. The G + C content of the genomic DNA was determined to be 61.5 mol % for strain SV1470(T) and 62.1 mol % for strain SV2184P(T). Both strains were found to have the same quinone system, with Q-10 as the major ubiquinone. The polar lipid profile of strain SV1470(T) was found to consist of phosphatidylcholine, phosphatidylglycerol, phosphatidylethanolamine, one unidentified phospholipid and one unidentified aminolipid, while that of strain SV2184P(T) consisted of phosphatidylcholine, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylmethylethanolamine, one unidentified aminolipid, one unidentified aminophospholipid and two unidentified phospholipids. DNA-DNA relatedness studies showed that the two strains belong to different genomic species. The strains were also distinguished using a combination of phenotypic properties. Based on the genotypic and phenotypic data, the novel species Microvirga makkahensis sp. nov. (type strain SV1470(T) = DSM 25394(T) = KCTC 23863(T) = NRRL-B 24875(T)) and Microvirga arabica sp. nov. (type strain SV2184P(T) = DSM 25393(T) = KCTC 23864(T) = NRRL-B 24874(T)) are proposed.Öğe New 5-methylisatin including thiocarbohydrazones: preparation, structure elucidation and antimicrobial activity(Springer, 2022) Yakan, Hasan; Cakmak, Sukriye; Buruk, Osman; Veyisoglu, Aysel; Muglu, Halit; Karakullukcu, Nalan TurkozNew 5-methylisatin including thiocarbohydrazones (1-5) have been synthesized. The chemical structure of synthesized compounds was elucidated with IR, H-1 NMR, C-13 NMR spectroscopic methods, and elemental analysis. Moreover, the synthesized compounds have been screened for antimicrobial activity. Their antibacterial activities were tested against Gram-positive (Bacillus subtilis ATCC 6623, Staphylococcus aureus ATCC 25923, Enterococcus faecalis ATCC 29212), Gram-negative (Escherichia coli ATCC 25922, Klebsiella pneumoniae ATCC 70060, Pseudomonas aeruginosa ATCC 27853), and fungal (Candida albicans ATCC 10231, Aspergillus niger ATCC 16404) microbial strains using the microdilution method. In the isatin series, particularly the compound 2 showed the best antimicrobial activity against E. faecalis strain with MIC values of 64 mu g/mL compared to other compounds. This high activity of compound 2 is due to the presence of two electron-donating methoxy groups in its structure. The remaining substituted compounds have shown good and moderate antimicrobial activity compared to standard drugs. The results may provide insights into the target compounds' structure-activity relationships, which may facilitate the development of pharmacological and biological applications for the target compounds. Graphical abstract New isatins bearing thiocarbohydrazone were synthesized. Structures of all com- pounds were elucidated with spectroscopic approaches. The antimicrobial activities of the tested compounds presented promising antimicrobial activity against the eight tested microorganisms.Öğe Nonomuraea cypriaca sp. nov., isolated from soil(Springer, 2021) Veyisoglu, AyselA novel actinobacterium, designated strain K274(T), was isolated from soil collected from Zafer Cape (Cape Apostolos Andreas), the easternmost tip of Cyprus on the Karpas peninsula, Magusa, Northern Cyprus, and a polyphasic approach was used for characterization of the strain. The isolate was found to have chemotaxonomic and morphological properties associated with members of the genus Nonomuraea. The strain has the highest similarity to Nonomuraea zeae DSM 100528(T) with 99.1% similarity value. In the phylogenetic dendogram based on 16S rRNA gene sequence, strain K274(T) was formed a distinct clade together N. zeae DSM 100528(T), 'Nonomuraea basaltis' 160415 (98.9% similarity), and 'Nonomuraea lycopersici' NEAU-DE8(1) (98.2% similarity). The genome sequence of strain K274(T) was 11.5 Mbp in size with a total of 11,848 protein-coding genes and 75 RNA genes. The genomic G + C content of the novel strain was 69.7 mol%. Both average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) results between the strain and phlyogenetic neighbours were well below the threshold value, and the novelty are supported by phenotypic and chemotaxonomic differences. Because of all these, strain K274(T) represents a novel species in the genus Nonomuraea, for which the name Nonomuraea cypriaca sp. nov. is proposed. The type strain is K274(T) (= DSM 45718(T) = KCTC 29095(T)).Öğe Phytomonospora cypria sp nov., isolated from soil(Springer, 2015) Sahin, Nevzat; Veyisoglu, Aysel; Tatar, Demet; Saygin, Hayrettin; Cetin, Demet; Guven, Kiymet; Klenk, Hans-PeterA Gram-stain positive actinobacterial strain, designated KT1403(T), was isolated from a soil sample, collected from Karpaz, Magusa, Northern Cyprus, and characterised using a polyphasic approach. Morphological characteristics and chemotaxonomic data indicated that the strain belongs to the genus Phytomonospora. The cell wall of the novel strain contained meso-diaminopimelic acid and galactose, glucose and mannose as the major sugars in whole cell hydrolysates. The polar lipids in the cell membrane were identified as diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, an unidentified aminophospholipid and three unidentified glycolipids. The predominant menaquinones were MK-10(H-6) and MK-10(H-4). The major fatty acids were found to be iso C-15:0 , anteiso C-15:0 and anteiso C-17:0. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain KT1403(T) belongs to the genus Phytomonospora with a sequence similarity of 99.73 % with Phytomonospora endophytica, the type species of the genus. DNA-DNA hybridization further differentiated strain KT1403(T) from its near phylogenetic neighbour, P. endophytica DSM 45386(T) (29.0 +/- A 2.2 % DNA relatedness). Therefore, it is proposed that strain KT1403(T) represents a novel species of the genus Phytomonospora, for which the name Phytomonospora cypria sp. nov. is proposed. The type strain is KT1403(T) (=KCTC 29479(T) = DSM 46767(T)).
