Kargi ve Osmancik Çevresindeki Yayla Topraklarinin Aktinomiset Çesitliligi
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Tarih
2020
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Bilecik Seyh Edebali Üniversitesi
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
Bu çalismada Çorum iline bagli Kargi (Eginönü), Abdullah ve Osmancik-Baspinar Karaca yaylalarindan alinan toprak örneklerinden izole edilen aktinomiset suslarinin 16S rRNA gen bölgesi analizlerine göre aktinomiset çesitliliginin saptanmasi amaçlandi. Dilüsyon plak tekniginin uygulandigi izolasyon çalismasinda 5 farkli seçici besiyeri kullanildi. Izolatlarin bes farkli kültür ortaminda kültürel karakterizasyonlari yapildi. Test izolatlarinin 16S rRNA gen bölgesinin PZR amplifikasyonu 27F ve 1525R primerleri ile gerçeklestirildi. Filogenetik agaçlar, MEGA 7.0 yazilim programinda neighbor-joining algoritmasiyla olusturuldu. 5 farkli izolasyon besiyerinden toplam 15 mikroorganizma seçildi ve 16S rRNA gen dizi analizine göre 15 izolatin, altisi Nocardia cinsi üyesi, besi Streptomyces cinsi üyesi, biri Haloactinopolyspora cinsi üyesi, biri Micromonospora cinsi üyesi, biri Nocardiopsis cinsi üyesi ve birinin de Rhodococcus cinsi üyesi oldugu tespit edildi.
In this study, it was aimed to determine the diversity of actinomycetes according to 16S rRNA analysis of actinomycete isolates obtained from soil samples taken from Kargi (Eginönü), Abdullah and Osmancik-Baspinar Karaca plateaus in Çorum Province. The isolates obtained were isolated using a dilution plate technique on 5 different selective media. Cultural characterizations of the isolates were carried out in five different culture media. PCR amplification belonging to 16S rRNA gene region of the isolates was realized out with primers of 27F and 1525R. Phylogenetic trees were formed with the neighbor-joining algorithm in MEGA 7.0 software. A total of 15 microorganisms were selected from 5 different isolation media and according to 16S rRNA gene sequence analysis of 15 isolates, six Nocardia isolates, five Streptomyces isolates, one Haloactinopolyspora isolate, one Micromonospora isolate, one Nocardiopsis isolate and one Rhodococcus isolate were determined.
In this study, it was aimed to determine the diversity of actinomycetes according to 16S rRNA analysis of actinomycete isolates obtained from soil samples taken from Kargi (Eginönü), Abdullah and Osmancik-Baspinar Karaca plateaus in Çorum Province. The isolates obtained were isolated using a dilution plate technique on 5 different selective media. Cultural characterizations of the isolates were carried out in five different culture media. PCR amplification belonging to 16S rRNA gene region of the isolates was realized out with primers of 27F and 1525R. Phylogenetic trees were formed with the neighbor-joining algorithm in MEGA 7.0 software. A total of 15 microorganisms were selected from 5 different isolation media and according to 16S rRNA gene sequence analysis of 15 isolates, six Nocardia isolates, five Streptomyces isolates, one Haloactinopolyspora isolate, one Micromonospora isolate, one Nocardiopsis isolate and one Rhodococcus isolate were determined.
Açıklama
ODMYO19003.18.001
Anahtar Kelimeler
Aktinomiset, Çesitlilik, Yayla Topragi, 16S rRNA geni
Kaynak
Bilecik Seyh Edebali Üniversitesi Fen Bilimleri Dergisi
WoS Q Değeri
Scopus Q Değeri
Cilt
7
Sayı
2
