Diversity and antimicrobial activity of culturable actinobacteria isolated from the sediment of Sarikum Lake
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Date
2021
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Taylor & Francis Ltd
Access Rights
info:eu-repo/semantics/openAccess
Abstract
In this study, actinobacteria isolated from Sarikum Lake sediment were characterized (Sinop-Turkey). A total of 48 actinobacteria were isolated using ten selective media with the dilution-plating method. The phylogenetic analysis according to 16S rRNA gene sequencing showed that 40% of the isolated strains belong to the widely distributed genus of Streptomyces, 36% belong to genus of Micromonospora, 24% of the isolates belong to rare genera such as Rhodococcus, Plantactinospora, Nonomuraea, Actinomadura and Streptosporangium. Most of the isolated strains belong to the genus Streptomyces (40%) and two isolates may be new species. All of the isolates were tested for antimicrobial activity; only 12 isolates exhibited antimicrobial activity. Nevertheless, 11 isolates were active against gram-positive, 5 were potential against gram-negative and no isolates had any effect against pathogenic fungi. All of the 48 isolates were analysed for genes encoding nonribosomal peptide synthetases (NRPS) and polyketide synthases (PKS). In all of the 48 isolates were detected NRPS sequences, PKS-I in 28 isolates and PKS-II in 22 isolates. PKS-I - PKS-II - NRPS genes were identified in 17 isolates.
Description
Keywords
Sarikum, actinobacteria, 16S rRNA, antimicrobial activity, NRPS, PKS-I and PKS-II
Journal or Series
Biotechnology & Biotechnological Equipment
WoS Q Value
Q4
Scopus Q Value
Q3
Volume
35
Issue
1
