THE MOLECULAR CHARACTERIZATION OF ESCHERICHIA COLI STRAINS ISOLATED FROM SEAWATER AND MUSSELS (MYTILUS GALLOPROVINCIALIS) IN SINOP PENINSULA, TURKEY

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dc.authoridAvsar, cumhur/0000-0002-4095-0022
dc.contributor.authorAvsar, Cumhur
dc.contributor.authorBerber, Ismet
dc.date.accessioned2025-03-23T19:47:35Z
dc.date.available2025-03-23T19:47:35Z
dc.date.issued2014
dc.departmentSinop Üniversitesi
dc.description.abstractIn this study, a total of 41 Escherichia coli strains were characterized according to phenotypic properties, whole cell and extracellular protein profiles, and randomly amplified polymorphic DNA (RAPD) method. The strains were isolated from seawater and mussels collected from 15 sampling sites in Sinop environs. The performing biochemical tests confirmed that all native isolates belonged to E. coli species. A numerical analysis of whole cell and extracellular protein profiles revealed that the strains showed high similarity with each other. A numerical analysis of the DNA band profiles obtained by using 2 different primers of 20 E. coli strains was carried out by using unweighted pair group method with arithmetic mean (UPGMA) method. A dendrogram generated by using RBM-6 primer revealed that 13 strains produced bands divided into 2 major clusters at similarity levels of >= 12%. However, a dendrogram generated by using COL-I primer displayed that 14 strains produced bands scattered into 2 major clusters at similarity levels of >= 26%. As a result, the numerical analysis of whole cell and extracellular protein profiles was enough for the differentiation of E. coli isolates at the species level, but not at strain level; however, RAPD-PCR technique might be provide fast and more useful discrimination at the strain level.
dc.identifier.endpage2487
dc.identifier.issn1018-4619
dc.identifier.issn1610-2304
dc.identifier.issue10
dc.identifier.scopus2-s2.0-84928111341
dc.identifier.scopusqualityN/A
dc.identifier.startpage2481
dc.identifier.urihttps://hdl.handle.net/11486/7385
dc.identifier.volume23
dc.identifier.wosWOS:000349805200015
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherParlar Scientific Publications (P S P)
dc.relation.ispartofFresenius Environmental Bulletin
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WOS_20250323
dc.subjectSeawater
dc.subjectE. coli
dc.subjectmussel
dc.subjectSDS-PAGE
dc.subjectRAPD-PCR
dc.titleTHE MOLECULAR CHARACTERIZATION OF ESCHERICHIA COLI STRAINS ISOLATED FROM SEAWATER AND MUSSELS (MYTILUS GALLOPROVINCIALIS) IN SINOP PENINSULA, TURKEY
dc.typeArticle

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