A novel amperometric H2O2 biosensor constructed by cress peroxidase entrapped on BiFeO3 nanoparticles

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dc.authoridKELES GUNER, EDA/0000-0002-4421-1315
dc.authoridOzdemir, Agah Oktay/0000-0003-4488-746X
dc.authoridCirak, Prof.Dr. Cagri/0000-0002-4717-8602
dc.authoridAltay, Ahmet/0000-0001-8120-8900
dc.contributor.authorCaglar, Bulent
dc.contributor.authorIcer, Fatih
dc.contributor.authorOzdokur, Kemal Volkan
dc.contributor.authorCaglar, Sema
dc.contributor.authorOzdemir, Agah Oktay
dc.contributor.authorGuner, Eda Keles
dc.contributor.authorBeser, Burcu Meryem
dc.date.accessioned2025-03-23T19:40:56Z
dc.date.available2025-03-23T19:40:56Z
dc.date.issued2021
dc.departmentSinop Üniversitesi
dc.description.abstractHerein, we synthesized ferromagnetic BiFeO3 nanoparticles with sizes of 20-60 nm and rhombohedral distorted perovskite structure without any co-formed impurity phases by sol-gel method. Structural, morphological, magnetic, electronic and textural properties of BiFeO3 nanoparticles were characterized by using XRD, SEM-EDX, TEM, FTIR, XPS, VSM, UV-DRS, PL and BET techniques. The peroxidase was isolated from the Cress (Lepidium sativum) and purified by using precipitation of ammonium sulfate and chromatographic techniques; gel filtration and ion exchange chromatography which is showed wide range of thermal stability, maximum activity in the acidic environment and close to the body temperature. Afterwards, a novel amperometric biosensor based on Cress peroxidase entrapped on BiFeO3 nanoparticles modified carbon paste electrode was prepared for the first time to sensitive detection of H2O2. The prepared H2O2 biosensor was operated with the aid of chomamperometry technique by applying -0.8 V vs Ag/AgCl electrode. The developed biosensor exhibited a linear response towards to H2O2 in the concentration range of 2.0 10(-7) to 1.0 10(-5) M under the optimal conditions and limit of detection and quantification were estimated as 7.0 10(-8)M (2.7 mu g L-1) and 2.0 10(-7) M (8.7 mu g L-1), respectively. The repeatability of the electrode was calculated as 19.2 for 2.0 10(-7) M (N = 7) and the accuracy was 81.2% for this concentration. In addition, the H2O2 biosensor exhibited excellent stability up to ten days. Finally, the sensor developed was used for the analysis of H2O2 in milk samples and satisfactory recoveries were obtained under the operating conditions.
dc.identifier.doi10.1016/j.matchemphys.2021.124287
dc.identifier.issn0254-0584
dc.identifier.issn1879-3312
dc.identifier.scopus2-s2.0-85100606232
dc.identifier.scopusqualityQ1
dc.identifier.urihttps://doi.org/10.1016/j.matchemphys.2021.124287
dc.identifier.urihttps://hdl.handle.net/11486/6462
dc.identifier.volume262
dc.identifier.wosWOS:000620873200002
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherElsevier Science Sa
dc.relation.ispartofMaterials Chemistry and Physics
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WOS_20250323
dc.subjectBiosensor
dc.subjectH2O2
dc.subjectBiFeO3 nanoparticles
dc.subjectCress peroxidase
dc.subjectAmperometry
dc.titleA novel amperometric H2O2 biosensor constructed by cress peroxidase entrapped on BiFeO3 nanoparticles
dc.typeArticle

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