Method validation and simultaneous determination of retinol, retinyl palmitate, β-Carotene, α-Tocopherol and vitamin C in Rat serum treated with 7,12 dimethylbenz[a]anthracene and plantago major L. By high- Performance liquid chromatography using diode-array detection

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dc.contributor.authorLevent, Abdulkadi
dc.contributor.authorOto, Gökhan
dc.contributor.authorEkin, Suat
dc.contributor.authorBerber, Ismet
dc.date.accessioned2025-03-23T19:17:02Z
dc.date.available2025-03-23T19:17:02Z
dc.date.issued2013
dc.departmentSinop Üniversitesi
dc.description.abstractA new and simple high-performance liquid chromatography method was developed and validated for the simultaneous determination of retinol, retinyl palmitate, β-carotene, α-tocopherol and vitamin C in rat serum treated with Plantago Major L. and 7,12 dimethylbenz[a]anthracene. High-performance liquid chromatography analysis was performed utilizing an Inertsil ODS3 reversed phase column with methanol-tetrahydrofuran-water as mobile phase under gradient conditions, at 1.5 mL min-1 flow rate and 25 °C. Diode-array detection was at 325, 450, 290 and 270 nm (retinol and retinyl palmitate), β-carotene, α-tocopherol and vitamin C, respectively and runnig time 18 min. The highperformance liquid chromatography assay and extraction procedure proposed are simple, rapid, sensitive and accurate. The method was then applied for the determination of retinol, retinyl palmitate, β-carotene, α-tocopherol and vitamin C in rat serum. Results of this study demonstrated that; at 60th day DMBA-treated group, there was a significant decrease in vitamin levels compared to the levels of control group. A significant increase was observed in vitamin levels of 7,12 dimethylbenz[a]anthracene+Plantago Major L.-treated group compared to the DMBA-treated group. Additionally, the results obtained in the study are found to be in agreement with data reported in the literature. © 2013 Bentham Science Publishers.
dc.identifier.doi10.2174/1386207311316020008
dc.identifier.endpage149
dc.identifier.issn1386-2073
dc.identifier.issue2
dc.identifier.pmid23176060
dc.identifier.scopus2-s2.0-84877983973
dc.identifier.scopusqualityQ3
dc.identifier.startpage142
dc.identifier.urihttps://doi.org/10.2174/1386207311316020008
dc.identifier.urihttps://hdl.handle.net/11486/4252
dc.identifier.volume16
dc.indekslendigikaynakScopus
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherBentham Science Publishers
dc.relation.ispartofCombinatorial Chemistry and High Throughput Screening
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_Scopus_20250323
dc.subject12 dimethylbenz[a]anthracene
dc.subjectHPLC
dc.subjectPlantago major l. 7
dc.subjectSerum
dc.subjectVitamin
dc.titleMethod validation and simultaneous determination of retinol, retinyl palmitate, β-Carotene, α-Tocopherol and vitamin C in Rat serum treated with 7,12 dimethylbenz[a]anthracene and plantago major L. By high- Performance liquid chromatography using diode-array detection
dc.typeArticle

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