MOLECULAR-BIOLOGICAL PROBLEMS OF DRUG DESIGN AND MECHANISM OF DRUG ACTION PROTECTIVE EFFECTS OF LIGANDROL AGAINST CISPLATIN-INDUCEDMOUSE MUSCLE CELL LINE TOXICITY

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dc.contributor.authorUstun, Zisan
dc.contributor.authorAkkan, Tamer
dc.contributor.authorGuner, Adem
dc.date.accessioned2026-04-25T14:19:49Z
dc.date.available2026-04-25T14:19:49Z
dc.date.issued2025
dc.departmentSinop Üniversitesi
dc.description.abstractCisplatin is an effective chemotherapeutic agent, but skeletal muscle dysfunction and fatigue are serious side effects after its administration. Ligandrol is a new nonsteroidal oral selective androgen receptor modulator used for muscle strengthening. The present study aimed to demonstrate the protective effects of ligandrol against cisplatin-induced muscle toxicity. Cytotoxic effects were determined by cell viability (MTT) and lactate dehydrogenase (LDH) assays in mouse muscle cell lines (C2C12). The oxidant/antioxidant status was demonstrated by total antioxidant capacity (TAC) and total oxidative status (TOS). Cell cycle analysis, DNA fragmentation, caspase 3/7 activities, lipid peroxidation, and intracellular calcium concentration levels were evaluated. Irritant effects were determined by the egg chorioallantoic membrane method. Our results showed that combination treatment with cisplatin of ligandrol significantly (p < 0.05) increased cell viability, TAC levels, and caspase 3/7 gene expression, while significantly (p < 0.05) reducing TOS, LDH release, DNA fragmentation, lipid peroxidation, and intracellular calcium concentration levels compared to the cisplatin alone. Ligandrol significantly ameliorated cisplatin-induced irritant effects. This result suggested that ligandrol had a significant protective effect against cisplatin-induced muscle toxicity.
dc.identifier.doi10.1007/s11094-025-03337-9
dc.identifier.endpage1785
dc.identifier.issn0091-150X
dc.identifier.issn1573-9031
dc.identifier.issue12
dc.identifier.orcid0000-0003-3295-3538
dc.identifier.scopus2-s2.0-105003202999
dc.identifier.scopusqualityQ4
dc.identifier.startpage1775
dc.identifier.urihttps://doi.org/10.1007/s11094-025-03337-9
dc.identifier.urihttps://hdl.handle.net/11486/8205
dc.identifier.volume58
dc.identifier.wosWOS:001467806500001
dc.identifier.wosqualityQ4
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherSpringer
dc.relation.ispartofPharmaceutical Chemistry Journal
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WOS_20260420
dc.subjectCisplatin
dc.subjectligandrol
dc.subjectmouse muscle cell line
dc.subjectoxidative stress
dc.subjecttoxicity
dc.titleMOLECULAR-BIOLOGICAL PROBLEMS OF DRUG DESIGN AND MECHANISM OF DRUG ACTION PROTECTIVE EFFECTS OF LIGANDROL AGAINST CISPLATIN-INDUCEDMOUSE MUSCLE CELL LINE TOXICITY
dc.typeArticle

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