Use of the Different Polyacrylamide Gel Electrophoresis (PAGE) Methods in the Characterization of Human Pathogenic Staphylococcus aureus Strains

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dc.authoridEkin, Suat/0000-0002-6502-5028
dc.contributor.authorBerber, Ismet
dc.contributor.authorAlan, Negmetullah
dc.contributor.authorEkin, Suat
dc.contributor.authorOnlu, Harun
dc.date.accessioned2025-03-23T19:47:55Z
dc.date.available2025-03-23T19:47:55Z
dc.date.issued2010
dc.departmentSinop Üniversitesi
dc.description.abstractIn the study, a total of 41 strains of Staphylococcus aureus including 38 clinical isolates and 3 reference strains were characterized according to biotyping, plasmid profiles and the numerical analysis of the protein profiles. The antibiogram results showed that the isolates were resistant against the tested antibiotics, except for vancomycin. Furthermore, plasmid profile analysis confirmed that the strains consisted of only one plasmid. Present findings indicated that the whole-cell and extracellular protein profiles obtained by using SDS-PAGE methods to be good typing tool for the differentiation of S. aureus strains at the species level, not strain level. However, Native-PAGE of whole-cell proteins was the most reliable and rapid method for differentiation between MRSA and ordinary S. aureus strains comparing to other applying PAGE techniques. In addition, it was determined that the same clone was responsible for most cases of MRSA and other S. aureus epidermic among surveyed hospitals. In conclusion, this study supposed that together application at least two different polyacrylamide gel electrophoresis (PAGE) techniques can be offer accurate and an effective approach to the investigation of taxonomic relationships within human pathogenic S. aureus strains.
dc.description.sponsorshipYuzuncu Yil University [2006-FBE-070]
dc.description.sponsorshipThis study was supported by a grant from the Scientific Research Projects Presidency of Yuzuncu Yil University (2006-FBE-070).
dc.identifier.endpage3185
dc.identifier.issn0970-7077
dc.identifier.issn0975-427X
dc.identifier.issue4
dc.identifier.scopus2-s2.0-77955537654
dc.identifier.scopusqualityQ4
dc.identifier.startpage3173
dc.identifier.urihttps://hdl.handle.net/11486/7448
dc.identifier.volume22
dc.identifier.wosWOS:000274581800101
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherAsian Journal Of Chemistry
dc.relation.ispartofAsian Journal of Chemistry
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WOS_20250323
dc.subjectNosocomial infections
dc.subjectStaphylococcus aureus
dc.subjectPAGE
dc.subjectprotein profiles
dc.titleUse of the Different Polyacrylamide Gel Electrophoresis (PAGE) Methods in the Characterization of Human Pathogenic Staphylococcus aureus Strains
dc.typeArticle

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