Determination of amikacin in human plasma by molecular imprinted SPR nanosensor

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dc.authoridAtar, Necip/0000-0001-8779-1412
dc.contributor.authorYola, Mehmet Lutfi
dc.contributor.authorAtar, Necip
dc.contributor.authorEren, Tanju
dc.date.accessioned2025-03-23T19:37:53Z
dc.date.available2025-03-23T19:37:53Z
dc.date.issued2014
dc.departmentSinop Üniversitesi
dc.description.abstractmolecular imprinting technique depends on the molecular recognition. It is polymerization method occuring around the target molecule. Thus this technique creates specific cavities in the cross-linked polymeric matrices. Amikacin (AMIK) is an important aminoglycoside (AG) antibiotic used to treat different types of bacterial infections. In this study, we prepared surface plasmon resonance (SPR) sensor for the determination of AMIK in human plasma. After the modification of gold surface of SPR chip was performed by allyl mercaptane, AMIK-imprinted poly(2-hydroxyethyl methacrylate-methacryloylamidoasparticacid) [p(HEMA-MAAsp)] film was generated on the allyl mercaptane modified gold surface. The non-modified and imprinted surfaces were characterized by Fourier transform infrared (FTIR) spectroscopy, atomic force microscopy (AFM) and contact angle measurements. The developed method was validated according to the ICH guideline. The linearity range and the detection limit were obtained as 0.01-0.15 mu g/mL and 0.0025 mu g/mL (4.3 Chi 10(-9) mol/L), respectively. The developed AMIK-imprinted nanosensor was successfully applied to the human plasma samples for the determination of AMIK. (C) 2014 Elsevier B.V. All rights reserved.
dc.identifier.doi10.1016/j.snb.2014.02.107
dc.identifier.endpage76
dc.identifier.issn0925-4005
dc.identifier.scopus2-s2.0-84897378048
dc.identifier.scopusqualityQ1
dc.identifier.startpage70
dc.identifier.urihttps://doi.org/10.1016/j.snb.2014.02.107
dc.identifier.urihttps://hdl.handle.net/11486/6040
dc.identifier.volume198
dc.identifier.wosWOS:000335580100011
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherElsevier Science Sa
dc.relation.ispartofSensors and Actuators B-Chemical
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WOS_20250323
dc.subjectAmikacin
dc.subjectSPR
dc.subjectMolecular imprinting
dc.subjectValidation
dc.subjectNanosensor
dc.titleDetermination of amikacin in human plasma by molecular imprinted SPR nanosensor
dc.typeArticle

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