Method Validation and Simultaneous Determination of Retinol, Retinyl Palmitate, β-Carotene, α-Tocopherol and Vitamin C in Rat Serum Treated with 7,12 Dimethylbenz[a]Anthracene and Plantago major L. by High-Performance Liquid Chromatography Using Diode-Array Detection

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dc.authoridLevent, Abdulkadir/0000-0001-5792-419X
dc.authoridEkin, Suat/0000-0002-6502-5028
dc.contributor.authorLevent, Abdulkadi
dc.contributor.authorOto, Gokhan
dc.contributor.authorEkin, Suat
dc.contributor.authorBerber, Ismet
dc.date.accessioned2025-03-23T19:48:47Z
dc.date.available2025-03-23T19:48:47Z
dc.date.issued2013
dc.departmentSinop Üniversitesi
dc.description.abstractA new and simple high-performance liquid chromatography method was developed and validated for the simultaneous determination of retinol, retinyl palmitate, beta-carotene, alpha-tocopherol and vitamin C in rat serum treated with Plantago Major L. and 7,12 dimethylbenz[a] anthracene. High-performance liquid chromatography analysis was performed utilizing an Inertsil ODS3 reversed phase column with methanol-tetrahydrofuran-water as mobile phase under gradient conditions, at 1.5 mL min(-1) flow rate and 25 degrees C. Diode-array detection was at 325, 450, 290 and 270 nm (retinol and retinyl palmitate), beta-carotene, alpha-tocopherol and vitamin C, respectively and runnig time 18 min. The high-performance liquid chromatography assay and extraction procedure proposed are simple, rapid, sensitive and accurate. The method was then applied for the determination of retinol, retinyl palmitate, beta-carotene, alpha-tocopherol and vitamin C in rat serum. Results of this study demonstrated that; at 60th day DMBA-treated group, there was a significant decrease in vitamin levels compared to the levels of control group. A significant increase was observed in vitamin levels of 7,12 dimethylbenz[a]anthracene+Plantago Major L.-treated group compared to the DMBA-treated group. Additionally, the results obtained in the study are found to be in agreement with data reported in the literature.
dc.description.sponsorshipYuzuncu Yil and University Scientific Research Foundation [2008-FED-B083]
dc.description.sponsorshipWe are grateful to Yuzuncu Yil and University Scientific Research Foundation (Project no. 2008-FED-B083) concerning the support of this research.
dc.identifier.endpage149
dc.identifier.issn1386-2073
dc.identifier.issue2
dc.identifier.pmid23176060
dc.identifier.scopusqualityQ3
dc.identifier.startpage142
dc.identifier.urihttps://hdl.handle.net/11486/7650
dc.identifier.volume16
dc.identifier.wosWOS:000317050800008
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakPubMed
dc.language.isoen
dc.publisherBentham Science Publ Ltd
dc.relation.ispartofCombinatorial Chemistry & High Throughput Screening
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WOS_20250323
dc.subject12 dimethylbenz[a]anthracene
dc.subjectHPLC
dc.subjectPlantago major L. 7
dc.subjectserum
dc.subjectvitamin
dc.titleMethod Validation and Simultaneous Determination of Retinol, Retinyl Palmitate, β-Carotene, α-Tocopherol and Vitamin C in Rat Serum Treated with 7,12 Dimethylbenz[a]Anthracene and Plantago major L. by High-Performance Liquid Chromatography Using Diode-Array Detection
dc.typeArticle

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