DEVELOPMENT OF NOVEL MARKERS, USING COMPUTATIONALLY EXTRACTED CLASSI TYPE EST-SSRS, IN WHEAT LEAF RUST FUNGUS Puccinia triticina

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dc.contributor.authorSipahi, Huelya
dc.contributor.authorYumurtaci, Aysen
dc.contributor.authorMert, Zafer
dc.date.accessioned2025-03-23T19:27:07Z
dc.date.available2025-03-23T19:27:07Z
dc.date.issued2015
dc.departmentSinop Üniversitesi
dc.description.abstractThis study focused on the development of EST-simple sequence repeats markers and the detection of their transferability and their utility for evaluating wheat leaf rust pathogen diversity. A total of 44,407 publicly available EST sequences derived from Puccinia triticina were computationally mined. Di-nucleotide repeat density covered the vast majority of assembled ESTs (45%). The tri-repeat motif (TCT) and penta-repeat motif (TTCTT) were the most repeated motif. A set of 103 Class I type sequences containing simple sequence repeats were further analyzed by BLASTX similarity. Nineteen primer pairs flanking regions of EST-SSRs were designed. Of the 19 primer pairs tested, 10 successfully amplified fragments. Their polymorphism was evaluated with 8 Puccinia triticina (Pt) single-uredinal isolates collected from the different regions of Turkey. These newly developed EST-SSR primer pairs can be implicated as stable markers for pathogen diversity analysis. It was also shown that some leaf rust EST-SSR markers were capable of cross-amplification in P. graminis f. sp. tritici.
dc.identifier.doi10.2298/GENSR1503917S
dc.identifier.endpage926
dc.identifier.issn0534-0012
dc.identifier.issn1820-6069
dc.identifier.issue3
dc.identifier.scopusqualityQ3
dc.identifier.startpage917
dc.identifier.urihttps://doi.org/10.2298/GENSR1503917S
dc.identifier.urihttps://hdl.handle.net/11486/4844
dc.identifier.volume47
dc.identifier.wosWOS:000369703300014
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.language.isoen
dc.publisherSerbian Genetics Soc
dc.relation.ispartofGenetika-Belgrade
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_WOS_20250323
dc.subjectPuccinia triticina
dc.subjectEST
dc.subjectsimple sequence repeats
dc.titleDEVELOPMENT OF NOVEL MARKERS, USING COMPUTATIONALLY EXTRACTED CLASSI TYPE EST-SSRS, IN WHEAT LEAF RUST FUNGUS Puccinia triticina
dc.typeArticle

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