DEVELOPMENT OF NOVEL MARKERS, USING COMPUTATIONALLY EXTRACTED CLASSI TYPE EST-SSRS, IN WHEAT LEAF RUST FUNGUS Puccinia triticina
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Tarih
2015
Yazarlar
Dergi Başlığı
Dergi ISSN
Cilt Başlığı
Yayıncı
Serbian Genetics Soc
Erişim Hakkı
info:eu-repo/semantics/openAccess
Özet
This study focused on the development of EST-simple sequence repeats markers and the detection of their transferability and their utility for evaluating wheat leaf rust pathogen diversity. A total of 44,407 publicly available EST sequences derived from Puccinia triticina were computationally mined. Di-nucleotide repeat density covered the vast majority of assembled ESTs (45%). The tri-repeat motif (TCT) and penta-repeat motif (TTCTT) were the most repeated motif. A set of 103 Class I type sequences containing simple sequence repeats were further analyzed by BLASTX similarity. Nineteen primer pairs flanking regions of EST-SSRs were designed. Of the 19 primer pairs tested, 10 successfully amplified fragments. Their polymorphism was evaluated with 8 Puccinia triticina (Pt) single-uredinal isolates collected from the different regions of Turkey. These newly developed EST-SSR primer pairs can be implicated as stable markers for pathogen diversity analysis. It was also shown that some leaf rust EST-SSR markers were capable of cross-amplification in P. graminis f. sp. tritici.
Açıklama
Anahtar Kelimeler
Puccinia triticina, EST, simple sequence repeats
Kaynak
Genetika-Belgrade
WoS Q Değeri
N/A
Scopus Q Değeri
Q3
Cilt
47
Sayı
3
