Identification of differentially expressed proteins in wheat after benzothiadiazole treatment

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dc.authoridGunel, Aslihan/0000-0001-5301-2628
dc.authoridAkkaya, Mahinur/0000-0002-0454-5898
dc.contributor.authorGunel, Aslihan
dc.contributor.authorAsbahi, Adnan
dc.contributor.authorOzgazi, Nese
dc.contributor.authorAkkaya, Mahinur S.
dc.date.accessioned2025-03-23T19:47:04Z
dc.date.available2025-03-23T19:47:04Z
dc.date.issued2012
dc.departmentSinop Üniversitesi
dc.description.abstractThe systemic acquired resistance (SAR) mechanism is stimulated by biological and chemical agents in response to pathogen infection as a part of the innate immunity response system of plants. The externally applied synthetic chemical, benzo-(1,2,3)-thiadiazole-7-carbothioic acid-S-methyl ester (BTH), is also known to induce a SAR response in plants. Studies identifying genes induced or suppressed by BTH are limited. Only few genes responding to BTH are determined. The focus of this preliminary study is to identify the gene products affected by BTH in wheat. A 2D-polyacrylamide gel electrophoresis (PAGE) analysis was carried out with BTH treated Triticum aestivum cv. Gerek-79 and mock treated samples. Following 2D-PAGE image analysis, the selected differentially expressed protein spots were identified by nanoLC-ESI-MS/MS. Among the 26 protein spots distinguished, five were found to be increased upon BTH treatment, another set of seven spots were absent in the control sample.Thus, they were apparent only in the gel of the BTH treated sample (+BTH), whereas five protein spots disappeared in the gel of the BTH treated plants (-BTH). Up-regulation of some proteins such as OEE2 (oxygen evolving enhancer protein) and COR (cold-responsive) LEA (late embryogenesis abundant)/RAB (responsive to abscisic acid, ABA) and down-regulation of some proteins such as RuBisCo LSU (large subunit), fructose 1, 6-biphosphate aldolase (AldP), methyl binding domain protein 6 (MBD6), and 3-isopropylmalate dehydrogenase are shown for the first time in BTH treatment of wheat.
dc.description.sponsorshipMETU; State Planning Organization of Turkey [DPT2004K120750]; TUBITAK (The Scientific and Technological Council of Turkey)
dc.description.sponsorshipThe authors are grateful to Prof. Dr. Ayse Ogan of Marmara University for allowing Dr. Gunel to use image analysing system; to METU Research Funds, to State Planning Organization of Turkey (DPT2004K120750) for grant supports; and to TUBITAK (The Scientific and Technological Council of Turkey) for the foreign graduate student scholarship to Adnan A.S. Al-Asbahi. The corresponding author is grateful to the president of Sinop University, Prof. Dr. Sevket Buyukhatipoglu, and president of Ahi Evran University, Prof. Dr. Selahattin Salman, for allowing Dr. Gunel's visits to METU. A special thank goes to Dr. Christof Rampitsch of Proteomics Department, Cereal Research Center, AAFC, Canada, for careful reading of the manuscript.
dc.identifier.doi10.1007/BF03356440
dc.identifier.endpage190
dc.identifier.issn1861-3829
dc.identifier.issn1861-3837
dc.identifier.issue5-6
dc.identifier.scopus2-s2.0-84871752390
dc.identifier.scopusqualityQ1
dc.identifier.startpage182
dc.identifier.urihttps://doi.org/10.1007/BF03356440
dc.identifier.urihttps://hdl.handle.net/11486/7269
dc.identifier.volume119
dc.identifier.wosWOS:000319033900004
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherSpringer Heidelberg
dc.relation.ispartofJournal of Plant Diseases and Protection
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WOS_20250323
dc.subjectBenzo-(1,2,3)-thiadiazole-7-carbothioic acid-S-methyl ester (BTH)
dc.subject2D-PAGE
dc.subjectnanoLC-ESI-MS/MS analysis
dc.subjectproteomics
dc.subjectwheat
dc.titleIdentification of differentially expressed proteins in wheat after benzothiadiazole treatment
dc.typeArticle

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