A novel and sensitive electrochemical DNA biosensor based on Fe@Au nanoparticles decorated graphene oxide

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dc.authoridAtar, Necip/0000-0001-8779-1412
dc.contributor.authorYola, Mehmet Lutfi
dc.contributor.authorEren, Tanju
dc.contributor.authorAtar, Necip
dc.date.accessioned2025-03-23T19:41:44Z
dc.date.available2025-03-23T19:41:44Z
dc.date.issued2014
dc.departmentSinop Üniversitesi
dc.description.abstractA novel and sensitive electrochemical biosensor for selective determination of DNA was developed based on Fe@Au nanoparticles (Fe@AuNPs) involving 2-aminoethanethiol (AET) functionalized graphene oxide (GO) (Fe@AuNPs-AETGO). Firstly, 5 '-TA CCG GGT GCT CGA GCC-(CH2)(3)-SH-3 ' single-stranded probe (ss-DNA) was immobilized on Fe@AuNPs-AETGO nanocomposite to form ssDNA-Fe@AuNPs-AETGO. Square wave voltammetry (SWV) was applied to monitor the DNA hybridization by basic blue 41 (BB41) as an electrochemical indicator. The DNA immobilization and hybridization on the film were studied by cyclic voltammetry (CV), SWV and electrochemical impedance spectroscopy (EIS). Under optimum conditions, the peak currents of BB41 were linear with the logarithm of the concentrations of complementary DNA (5 '-AT GGC CCA CGA GCT CGA-(CH2)(3)-SH-3) from 1.0 x 10(-14) to 1.0 x 10(-8) M with a detection limit of 2.0 x 10(-15) M. The biosensor also showed high selectivity to one-base, two-base and three-base mismatched DNA. Compared with the other electrochemical DNA biosensors, the proposed biosensor showed its own performance of simplicity, good stability and high sensitivity. (C) 2014 Elsevier Ltd. All rights reserved.
dc.identifier.doi10.1016/j.electacta.2014.01.074
dc.identifier.endpage47
dc.identifier.issn0013-4686
dc.identifier.issn1873-3859
dc.identifier.scopus2-s2.0-84894124482
dc.identifier.scopusqualityQ1
dc.identifier.startpage38
dc.identifier.urihttps://doi.org/10.1016/j.electacta.2014.01.074
dc.identifier.urihttps://hdl.handle.net/11486/6629
dc.identifier.volume125
dc.identifier.wosWOS:000335424300005
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherPergamon-Elsevier Science Ltd
dc.relation.ispartofElectrochimica Acta
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WOS_20250323
dc.subjectGraphene oxide
dc.subjectFe@Au nanoparticles
dc.subjectSquare wave voltammetry
dc.subjectElectrochemical impedance spectroscopy
dc.subjectDNA biosensor
dc.titleA novel and sensitive electrochemical DNA biosensor based on Fe@Au nanoparticles decorated graphene oxide
dc.typeArticle

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