Enhanced biodiesel properties of Auxenochlorella sp. using chemical mutagenesis and Tralkoxydim

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dc.authoridAltinbas, Mahmut/0000-0003-3946-741X
dc.authoridPolat, Ece/0000-0001-7173-9126
dc.contributor.authorPolat, Ece
dc.contributor.authorAltinbas, Mahmut
dc.date.accessioned2025-03-23T19:34:44Z
dc.date.available2025-03-23T19:34:44Z
dc.date.issued2023
dc.departmentSinop Üniversitesi
dc.description.abstractMutagenesis in microbial strains might result in robust strains with enhanced biofuel properties. Besides that, oleaginous microalgae, Auxenochlorella sp. with enhanced biomass and lipid content, can be a strong candidate for biodiesel production. In this context, a chemical mutagen called ethyl methane sulfonate (EMS) was screened at different concentrations. Considering that a lower survival rate can result in a higher mutation rate, an EMS concentration of 0.25M with 13 mins exposure time was selected as the optimum mutation condition. An acetyl-coenzyme A carboxylase (ACCase) inhibitor herbicide, tralkoxydim, was used as a selective environment to choose the mutant strains. Using an ACCase inhibitor herbicide would maintain a more desirable mutant strain that had the enhanced characteristics of biodiesel. Multi-scale comparisons between selected mutant strains and a wild strain revealed that by the time there was a remarkable decrease of 12.6% in the lipid content of the wild strain under the addition of Tralkoxydim (34.8%) compared to 39.8% in wild type, the lipid contents of the mutant strains remained higher. A remarkable increase by 1.50-fold and 1.33-fold in biodiesel fuel properties for Tralkoxydim-treated strains emphasizes that the ACCase synthesis pathway for lipid production is influenced by the stress factor that reaches the European biodiesel standards.
dc.identifier.doi10.1080/15435075.2022.2149269
dc.identifier.endpage1148
dc.identifier.issn1543-5075
dc.identifier.issn1543-5083
dc.identifier.issue11
dc.identifier.scopus2-s2.0-85142633226
dc.identifier.scopusqualityQ2
dc.identifier.startpage1137
dc.identifier.urihttps://doi.org/10.1080/15435075.2022.2149269
dc.identifier.urihttps://hdl.handle.net/11486/5720
dc.identifier.volume20
dc.identifier.wosWOS:000889307100001
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherTaylor & Francis Inc
dc.relation.ispartofInternational Journal of Green Energy
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WOS_20250323
dc.subjectBiodiesel
dc.subjectAuxenochlorella sp
dc.subjectAcetyl coenzyme a carboxylase
dc.subjectfatty acid profile
dc.subjectTralkoxydim
dc.titleEnhanced biodiesel properties of Auxenochlorella sp. using chemical mutagenesis and Tralkoxydim
dc.typeArticle

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