Rapid Analysis of Polycyclic Aromatic Hydrocarbons in Water Samples Using an Automated On-line Two-Dimensional Liquid Chromatography

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dc.authoridArmutcu, Canan/0000-0002-0920-2843
dc.authoridCorman, Mehmet Emin/0000-0001-9062-4201
dc.authoridUzun, Lokman/0000-0002-3971-7725
dc.authoridKarasu, Tunca/0000-0001-5564-5883
dc.contributor.authorArmutcu, Canan
dc.contributor.authorOzgur, Erdogan
dc.contributor.authorKarasu, Tunca
dc.contributor.authorBayram, Engin
dc.contributor.authorUzun, Lokman
dc.contributor.authorCorman, Mehmet Emin
dc.date.accessioned2025-03-23T19:44:01Z
dc.date.available2025-03-23T19:44:01Z
dc.date.issued2019
dc.departmentSinop Üniversitesi
dc.description.abstractTwo-dimensional HPLC (2D-HPLC) recently has received great attention due to providing high resolving power and higher peak capacities than that of 1D-HPLC, especially dealing with a wide spectrum of sample matrices containing several components. In this work, an on-line heart-cutting two-dimensional liquid chromatography (2D-LC) method was developed using monolithic columns coupled with reversed-phase liquid chromatography (RPLC). 2D-HPLC was successfully carried out using affinity-based monolithic columns at first dimension (20 cm x 4.6 mm I.D.) followed by a Pinnacle II PAH column (50 mm x 4.6 mm I.D.) at the second dimension. Furthermore, good linearity was observed for the correlation of the benzo[a]pyrene (BaP) molecule against the peak areas (R-2 = 0.994) in the concentration range of 0.01-1.0 ng/mL in 30 min. The limit of detection and the limit of quantification were found to be 4.0 pg/mL and 12.0 pg/mL, respectively. Both the intra-day and inter-day precision at 0.01 and 0.1 ng/mL spiked concentrations were below than 2.35% RSD whereas the means of the recovery data of the BaP from the water samples were found to be in the range of 93.71-98.65%. These results demonstrate that the 2D-HPLC system, developed by the combination of the P(HEMA-MAPA) column and Pinnacle II PAH column, is reliable, stable, and well qualified in the extraction of polycyclic aromatic hydrocarbons from the water samples.
dc.description.sponsorshipScientific and Technological Research Council of Turkey [TUBITAK-KBAG-214Z236]
dc.description.sponsorshipThis study was supported by the Scientific and Technological Research Council of Turkey with grant number TUBITAK-KBAG-214Z236. L.U. would like to thank Turkish Academy of Sciences (TUBA-GEBIP) for their invaluable support.
dc.identifier.doi10.1007/s11270-019-4306-7
dc.identifier.issn0049-6979
dc.identifier.issn1573-2932
dc.identifier.issue10
dc.identifier.scopus2-s2.0-85074193896
dc.identifier.scopusqualityQ2
dc.identifier.urihttps://doi.org/10.1007/s11270-019-4306-7
dc.identifier.urihttps://hdl.handle.net/11486/6850
dc.identifier.volume230
dc.identifier.wosWOS:000492941400002
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherSpringer International Publishing Ag
dc.relation.ispartofWater Air and Soil Pollution
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WOS_20250323
dc.subjectTwo-dimensional high-performance liquid chromatography
dc.subjectbenzo[a]pyrene (BaP)
dc.subjectmonolithic column
dc.subjectwater samples
dc.titleRapid Analysis of Polycyclic Aromatic Hydrocarbons in Water Samples Using an Automated On-line Two-Dimensional Liquid Chromatography
dc.typeArticle

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