Two-Way Bacterial Detoxifiers Obtained from Naturally Boron-Rich Sediments in Türkiye: a Study on Intracellular Boron Accumulation and Arsenite Biotransformation

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dc.contributor.authorAvsar, Cumhur
dc.date.accessioned2026-04-25T14:20:06Z
dc.date.available2026-04-25T14:20:06Z
dc.date.issued2026
dc.departmentSinop Üniversitesi
dc.description.abstractBoron and arsenic are elements that can acquire harmful quantities as a result of both natural and human activity. This research assessed boron tolerance, intracellular boron accumulation, resistance to arsenite [As(III)] and arsenate [As(V)], arsenite transformation capabilities, and genetic characterization of 31 strains taken from Turkey, a country noted for its high boron concentration. Experimental testing indicated that the majority of the strains exhibited tolerance to boron dosages of 150-200 mM, while a minority tolerated levels of 250-300 mM. In the intracellular boron accumulation experiments, strains 8 (1.32 +/- 0.05 mg/L) and 31 (0.94 +/- 0.03 mg/L) from the Lysinibacillus genus displayed high activity. The MIC and MBC techniques for both arsenite and arsenate were applied to assess the resistance state. Furthermore, five species, including strain 8 from the genus Lysinibacillus, were discovered to oxidize As(III) to As(V). Furthermore, aioA, a gene implicated in arsenite oxidation, was tested via PCR; however, it was not discovered in any of the strains. Nonetheless, the transformation of As(III) was phenotypically verified using a colorimetric approach. Phylogenetic analysis based on 16S rRNA and AFLP data was also performed and indicated that most bacteria belonged to the genera Lysinibacillus and Bacillus, while a few were firmly connected to Enterobacter and Enterococcus. The findings suggested that strain 8, a member of Lysinibacillus spp., is a promising candidate indigenous strain for bioremediation purposes to detoxify settings abundant in boron minerals from excess boron and arsenic.
dc.description.sponsorshipSinop University Scientific Research Project Coordination Unit [FEF-1901-24-004]
dc.description.sponsorshipThis research has been supported by Sinop University Scientific Research Project Coordination Unit. Project Number: FEF-1901-24-004, 2024-2025.
dc.identifier.doi10.1080/01490451.2025.2573813
dc.identifier.endpage338
dc.identifier.issn0149-0451
dc.identifier.issn1521-0529
dc.identifier.issue3
dc.identifier.orcid0000-0002-4095-0022
dc.identifier.scopus2-s2.0-105019066169
dc.identifier.scopusqualityQ1
dc.identifier.startpage326
dc.identifier.urihttps://doi.org/10.1080/01490451.2025.2573813
dc.identifier.urihttps://hdl.handle.net/11486/8364
dc.identifier.volume43
dc.identifier.wosWOS:001593201100001
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.institutionauthorAvsar, Cumhur
dc.language.isoen
dc.publisherTaylor & Francis Inc
dc.relation.ispartofGeomicrobiology Journal
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WOS_20260420
dc.subjectLysinibacillus spp
dc.subjectbioremediation
dc.subjectarsenite oxidation
dc.subjectheavy metal detoxification
dc.titleTwo-Way Bacterial Detoxifiers Obtained from Naturally Boron-Rich Sediments in Türkiye: a Study on Intracellular Boron Accumulation and Arsenite Biotransformation
dc.typeArticle

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