Polymeric nanoparticles tryptophan-graft-p(HEMA): a study on synthesis, characterization, and toxicity

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dc.authoridAkgol, Sinan/0000-0002-8528-1854
dc.authoridGUNER, ADEM/0000-0003-3295-3538
dc.authoridKarabay Yavasoglu, N.Ulku/0000-0002-7483-0184
dc.contributor.authorGuler, Cem
dc.contributor.authorGulcemal, Suleyman
dc.contributor.authorGuner, Adem
dc.contributor.authorAkgol, Sinan
dc.contributor.authorYavasoglu, N. Ulku Karabay
dc.date.accessioned2025-03-23T19:44:38Z
dc.date.available2025-03-23T19:44:38Z
dc.date.issued2023
dc.departmentSinop Üniversitesi
dc.description.abstractPoly-hydroxyethyl methacrylate [p(HEMA)] is one of the most widely used polymers in different biomedical applications because it is a biocompatible and a biodegradable material. Tryptophan (Trp) is a biocompatible, antioxidant, and anti-inflammatory amino acid. Trp modification contributes to the more effective use of nanoparticles in cancer therapy. The aim of this study was to synthesize polymeric nanoparticles tryptophan-graft-poly(HEMA) [Trp-g-p(HEMA)] and assess characterization and toxicity/biocompatibility potential of it in terms of using a drug carrier. The nanoparticles were synthesized with surfactant-free emulsion polymerization and grafting technique and the grafting efficiency was found as 78.65 +/- 2.48%. The characterization of the nanoparticles was performed by FT-IR spectroscopy, zeta analysis, scanning electron microscopy, atomic force microscopy, and swelling test. The nanopolymers had the spectra from 750 to 4000 cm(-1) and characteristic peaks of stretching bands, 164.1 +/- 29.2 nm average size, - 10.2 +/- 8.7 mV surface charge, smooth surface, and nearly spherical shape. The swelling ratios of them were estimated as 79.52 +/- 0.86% in d.w. and 93.33 +/- 2.32% in PBS at 25 degrees C, 35.71 +/- 0.62% in d.w., and 42.86 +/- 0.64% in PBS at 37 degrees C. The nanoparticles did not induce cytotoxicity, oxidative stress generation, and genotoxicity on human healthy lymphocyte cells. Trp-g-p(HEMA) had hemocompatible properties. We found no irritant effect in the HET-CAM test. The acute oral LD50 value of the nanopolymers was > 2000 mg/kg body weight on BALB/c mice. We announce that the polymeric nanoparticles Trp-g-p(HEMA) is a biocompatible material and has potential to use as a drug carrier for oral, intravenous, and ocular administrations.
dc.description.sponsorshipEge University, Scientific Research Project [2017/FEN/028]
dc.description.sponsorshipThis study was supported by Ege University, Scientific Research Project (Project Number is 2017/FEN/028).
dc.identifier.doi10.1007/s00289-022-04607-2
dc.identifier.endpage10996
dc.identifier.issn0170-0839
dc.identifier.issn1436-2449
dc.identifier.issue10
dc.identifier.scopus2-s2.0-85142701300
dc.identifier.scopusqualityQ1
dc.identifier.startpage10973
dc.identifier.urihttps://doi.org/10.1007/s00289-022-04607-2
dc.identifier.urihttps://hdl.handle.net/11486/6985
dc.identifier.volume80
dc.identifier.wosWOS:000889039200008
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherSpringer
dc.relation.ispartofPolymer Bulletin
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WOS_20250323
dc.subjectTryptophan-graft-p(HEMA)
dc.subjectPolymeric nanoparticle
dc.subjectCytotoxicity
dc.subjectGenotoxicity
dc.subjectHemolysis
dc.subjectDrug carrier
dc.titlePolymeric nanoparticles tryptophan-graft-p(HEMA): a study on synthesis, characterization, and toxicity
dc.typeArticle

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