Structural characterization of new zinc(ii) complexes with N2O2 chelating thiosemicarbazidato ligands; investigation of the relationship between their DNA interaction and in vitro antiproliferative activity towards human cancer cells

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dc.authoridULKUSEVEN, BAHRI/0000-0001-6342-1505
dc.authoridKaya, Busra/0000-0003-2706-172X
dc.contributor.authorKaya, Busra
dc.contributor.authorYilmaz, Zehra Kubra
dc.contributor.authorSahin, Onur
dc.contributor.authorAslim, Belma
dc.contributor.authorUlkuseven, Bahri
dc.date.accessioned2025-03-23T19:35:42Z
dc.date.available2025-03-23T19:35:42Z
dc.date.issued2020
dc.departmentSinop Üniversitesi
dc.description.abstractTwo new zinc(ii) complexes were synthesized by condensation of 4,4,4-trifluoro-1-(2-thienyl)-1,3-butanedione-S-methyl-thiosemicarbazone with salicylaldehyde or 4-methoxy-salicylaldehyde. Structures of the N2O2-chelate complexes, Zn1 and Zn2, were explicated by IR, H-1 NMR, ESI-MS and X-ray crystallography. Electronic spectral analysis was performed to assign the nature of the interaction between the complexes and calf thymus DNA (CT-DNA). DNA cleavage activities of the complexes were investigated by gel electrophoresis (pBR322 DNA). The results indicated that the complexes bind to CT-DNA via an intercalation mode and the binding of Zn2 is stronger than that of Zn1. Relatively high concentrations of the zinc complexes were found to encourage the cleavage of DNA from supercoiled form (Form I) to nicked circular form (Form II). The antiproliferative activity of the complexes was determined against human colorectal adenocarcinoma (HT-29) and human cervical carcinoma (HeLa) cell lines by MTT assay. The tests revealed that the complex Zn2 exhibited a higher antiproliferative effect than Zn1 on HT-29 and HeLa cells, analogical to the data from DNA interaction experiments. The studies demonstrated that complex Zn2 could be a good candidate as a chemotherapeutic drug targeting DNA.
dc.description.sponsorshipScientific Research Projects Coordination Unit of Istanbul University-Cerrahpasa
dc.description.sponsorshipThis work was supported by the Scientific Research Projects Coordination Unit of Istanbul University-Cerrahpasa. The authors acknowledge the Scientific and Technological Research Application and Research Center, Sinop University, Turkey, for the use of the Bruker D8-QUEST diffractometer.
dc.identifier.doi10.1039/d0nj02149k
dc.identifier.endpage9320
dc.identifier.issn1144-0546
dc.identifier.issn1369-9261
dc.identifier.issue22
dc.identifier.scopus2-s2.0-85086180025
dc.identifier.scopusqualityQ2
dc.identifier.startpage9313
dc.identifier.urihttps://doi.org/10.1039/d0nj02149k
dc.identifier.urihttps://hdl.handle.net/11486/5910
dc.identifier.volume44
dc.identifier.wosWOS:000540929200021
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherRoyal Soc Chemistry
dc.relation.ispartofNew Journal of Chemistry
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WOS_20250323
dc.subjectS-Substituted Isothiosemicarbazides
dc.subjectPbr322 Plasmid Cleavage
dc.subjectMinor-Groove Binders
dc.subjectCopper(Ii) Complexes
dc.subjectAntimicrobial Activity
dc.subjectMolecular Docking
dc.subjectNi(Ii) Complexes
dc.subjectZn(Ii) Complexes
dc.subjectCoordination Chemistry
dc.subjectNickel(Ii) Complexes
dc.titleStructural characterization of new zinc(ii) complexes with N2O2 chelating thiosemicarbazidato ligands; investigation of the relationship between their DNA interaction and in vitro antiproliferative activity towards human cancer cells
dc.typeArticle

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