Molecularly imprinted cryogel cartridges for the specific filtration and rapid separation of interferon alpha

Basit Öğe Kaydı
dc.authoridArmutcu, Canan/0000-0002-0920-2843
dc.authoridUzun, Lokman/0000-0002-3971-7725
dc.authoridCorman, Mehmet Emin/0000-0001-9062-4201
dc.contributor.authorCorman, Mehmet Emin
dc.contributor.authorArmutcu, Canan
dc.contributor.authorOzkara, Serpil
dc.contributor.authorUzun, Lokman
dc.contributor.authorDenizli, Adil
dc.date.accessioned2025-03-23T19:35:44Z
dc.date.available2025-03-23T19:35:44Z
dc.date.issued2015
dc.departmentSinop Üniversitesi
dc.description.abstractIn this study, we synthesised specific filtration cartridges with selective recognition sites for target molecules and used them to separate interferon alpha-2b from aqueous solutions. We combined molecular imprinting technology with cryogel to achieve specific and rapid filtration of interferon alpha-2b through the macroporous structure of a cryogel network. Recombinant interferon alpha-2b-imprinted poly(2-hydroxyethyl methacrylate-N-methacryloyl-L-tryptophan) P(HEMATrp)/alpha-2bIFN cryogels were synthesised via free-radical bulk polymerisation under partially frozen conditions. The interferon alpha-2b filtration conditions were subsequently optimised with respect to factors such as pH, initial concentration, temperature, centrifugation speed, salt concentration and type and the amount of precomplex incorporated. Selectivity experiments were conducted in respect to isoelectric point as well as size of the competitor proteins under both uncompetitive and competitive conditions. The relative selectivity coefficients of the specific filtration cartridge in respect to isoelectronic points for interferon/ IgG, interferon/HSA and interferon/insulin pairs were 3.72, 7.10 and 10.68 times greater than the coefficient of a non-imprinted [P(HEMATrp)] filtration cartridge, respectively. Similarly, the relative selectivity coefficients of the specific filtration cartridge in respect to competitor size for interferon/ lysozyme, interferon/myoglobin and interferon/carbonic anhydrase pairs were calculated as 7.58, 10.40 and 11.68 under uncompetitive conditions whereas those values under competitive conditions were calculated as 1.08, 1.05 and 1.34, respectively. The results indicated that specific filtration cartridges developed could repeatedly adsorb interferon alpha-2b with a short separation time without any significant decrease in the adsorption capacity even if competitive conditions were conducted.
dc.identifier.doi10.1039/c5ra07307c
dc.identifier.endpage45026
dc.identifier.issn2046-2069
dc.identifier.issue56
dc.identifier.scopus2-s2.0-84930227250
dc.identifier.scopusqualityQ1
dc.identifier.startpage45015
dc.identifier.urihttps://doi.org/10.1039/c5ra07307c
dc.identifier.urihttps://hdl.handle.net/11486/5923
dc.identifier.volume5
dc.identifier.wosWOS:000354965300034
dc.identifier.wosqualityQ2
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherRoyal Soc Chemistry
dc.relation.ispartofRsc Advances
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WOS_20250323
dc.subjectHydrophobic Interaction Chromatography
dc.subjectUltrafiltration Uf Membrane
dc.subjectNatural Organic-Matter
dc.subjectProtein Separation
dc.subjectHuman Serum
dc.subjectAdsorption
dc.subjectAffinity
dc.subjectPerformance
dc.subjectPolymers
dc.subjectNanoparticles
dc.titleMolecularly imprinted cryogel cartridges for the specific filtration and rapid separation of interferon alpha
dc.typeArticle

Dosyalar

Koleksiyon

WoS İndeksli Yayınlar Koleksiyonu
Scopus İndeksli Yayınlar Koleksiyonu