IN VITRO EFFECTS OF ANTIOXIDANT AND PROAPOPTOTIC ACTIVITIES OF THYMOQUINONE IRON COMPLEX

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dc.authoridAlizade, Ares/0000-0003-0334-8152
dc.contributor.authorOzbolat, Guluzar
dc.contributor.authorAlizade, Ares
dc.date.accessioned2025-03-23T19:26:44Z
dc.date.available2025-03-23T19:26:44Z
dc.date.issued2022
dc.departmentSinop Üniversitesi
dc.description.abstractIntroduction: This study aimed to investigate the proapoptotic and antioxidant effects of the Thymoquinone (TQ) iron complex on the SW480 cell line. This study investigates the proapoptotic and antioxi-dant effects of the TQ iron complex on the SW480 cell line. Material and Methods: The SW480 cells were routinely cultured in a medium for 48 h. and incubated at 37 degrees C in a 5% CO2 in the air. After the incubation period, the cells were washed with buffer, and 100 ml of the denaturing lysis buffer per 0.5 was added to 2x10(7) cells for 15 min, and supernatants were tak-en. ELISA test was used to examine the expression and activity of GADD153, Wee1, cleaved Caspase-3, Bax, GRP78, and Bcl-2 proteins in SW480 cells. In this study, to measure activities of total antioxidant capacity (TAS), catalase (CAT), total oxidant capacity (TOS), and superox-ide dismutase (SOD) activities were investigated by the ELISA method in cell lines SW480 treated with the TQ iron complex. Results: ELISA test results indicated that the activities of apoptotic proteins Bax, Wee1 Caspase-3, GADD153, GRP78, and Bcl-2 in human SW480 cell lines were significantly increased in the 48-hour treatment. Conclusion: Our results of this study demonstrated that in untreated cultures, high TAS, SOD and CAT ac-tivities were found in SW480 cell lines than in control cell lines.
dc.identifier.doi10.30621/jbachs.998974
dc.identifier.endpage85
dc.identifier.issn2458-8938
dc.identifier.issn2564-7288
dc.identifier.issue2
dc.identifier.scopusqualityN/A
dc.identifier.startpage81
dc.identifier.trdizinid1275086
dc.identifier.urihttps://doi.org/10.30621/jbachs.998974
dc.identifier.urihttps://search.trdizin.gov.tr/tr/yayin/detay/1275086
dc.identifier.urihttps://hdl.handle.net/11486/4771
dc.identifier.volume6
dc.identifier.wosWOS:000809667400010
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakTR-Dizin
dc.language.isoen
dc.publisherDokuz Eylul Univ Inst Health Sciences
dc.relation.ispartofJournal of Basic and Clinical Health Sciences
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/openAccess
dc.snmzKA_WOS_20250323
dc.subjectTQ
dc.subjectiron chelation
dc.subjectbax
dc.subjectcaspase-3
dc.subjectbcl-2
dc.subjectiron
dc.subjectdrug
dc.titleIN VITRO EFFECTS OF ANTIOXIDANT AND PROAPOPTOTIC ACTIVITIES OF THYMOQUINONE IRON COMPLEX
dc.typeArticle

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