Histidine-epoxy-activated sepharose beads embedded poly (2-hydroxyethyl methacrylate) cryogels for pseudobiospecific adsorption of human immunoglobulin G

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dc.authoridCorman, Mehmet Emin/0000-0001-9062-4201
dc.authoridArmutcu, Canan/0000-0002-0920-2843
dc.contributor.authorCorman, Mehmet Emin
dc.contributor.authorArmutcu, Canan
dc.contributor.authorBereli, Nilay
dc.contributor.authorElkak, Assem
dc.date.accessioned2025-03-23T19:34:55Z
dc.date.available2025-03-23T19:34:55Z
dc.date.issued2017
dc.departmentSinop Üniversitesi
dc.description.abstractThe use of highly purified immunoglobulin became among the most powerful adopted strategies in therapeutic trials nowadays. Their role as immunomodulatory and anti-inflammatory agents has widened their scope of use. A novel continuous supermacroporous monolithic cryogels embedded with histidine-epoxy-activated-sepharose beads were synthetized as a new monolithic adsorbents for the separation of immunoglobulin G from human serum. The histidine-epoxy-activated-sepharose beads were embedded into the 2-hydroxyethyl methacrylate (HEMA) cryogels present in frozen aqueous solution inside a plastic syringe. The microstructure morphology of the cryogels was characterized by swelling measurement and scanning electron microscopy. The adsorption of human IgG on the histidine-epoxy-activated-sepharose beads pHEMA cryogels appeared to follow the Langmuir-Freundlich adsorption isotherm model. The maximum IgG adsorption was observed at 4 degrees C and pH 7.4 and was found to be 26.95mg/g of cryogel which is close to that obtained experimentally (24.49mg/g). The cryogels were used for several adsorption-desorption cycles without any negligible decrease in their adsorption capacity.
dc.identifier.doi10.1080/10601325.2017.1381921
dc.identifier.endpage922
dc.identifier.issn1060-1325
dc.identifier.issn1520-5738
dc.identifier.issue12
dc.identifier.scopus2-s2.0-85032020576
dc.identifier.scopusqualityQ2
dc.identifier.startpage915
dc.identifier.urihttps://doi.org/10.1080/10601325.2017.1381921
dc.identifier.urihttps://hdl.handle.net/11486/5768
dc.identifier.volume54
dc.identifier.wosWOS:000415990100007
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherTaylor & Francis Inc
dc.relation.ispartofJournal of Macromolecular Science Part A-Pure and Applied Chemistry
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WOS_20250323
dc.subjecthistidine-sepharose
dc.subjectpHEMA
dc.subjectcryogels
dc.subjectpseudobioaffinity
dc.subjecthuman IgG
dc.titleHistidine-epoxy-activated sepharose beads embedded poly (2-hydroxyethyl methacrylate) cryogels for pseudobiospecific adsorption of human immunoglobulin G
dc.typeArticle

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