A novel impedimetric biosensor based on graphene oxide/gold nanoplatform for detection of DNA arrays

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dc.authoridUstundag, Zafer/0000-0002-5550-106X
dc.authoridAtar, Necip/0000-0001-8779-1412
dc.contributor.authorGupta, Vinod Kumar
dc.contributor.authorYola, Mehmet Lutfi
dc.contributor.authorQureshi, Munewar Saeed
dc.contributor.authorSolak, Ali Osman
dc.contributor.authorAtar, Necip
dc.contributor.authorUstundag, Zafer
dc.date.accessioned2025-03-23T19:37:54Z
dc.date.available2025-03-23T19:37:54Z
dc.date.issued2013
dc.departmentSinop Üniversitesi
dc.description.abstractA highly sensitive method for detection of DNA hybridization was developed. This method was based on the modification of glassy carbon electrode with gold nanoparticles (AuNPs) involving p-aminothiophenol (ATP) functionalized graphene oxide (GO). This GO was used as a platform for impedimetric genosensing using 5'-TA GGG CCA CTT GGA CCT-(CH2)(3)-SH-3' single-stranded probe (ss-DNA), 5'-AGG TCC AAG TGG CCC TA-3'(target DNA), 5'-SH-C-6-TAG GGC CA-3'(non-complementary-1) and 5'-SH-C-6-TGC CCG TTA CG 3-' (non-complementary-2) oligonucleotide sequences. The film exhibited excellent properties for immobilizing DNA probes and sensing DNA hybridization. The DNA immobilization and hybridization on the film were studied by cyclic voltammetry (CV), and electrochemical impedance spectroscopy (EIS), and found that the charge transfer resistance (R-ct) of the electrode increased with the concentration of the target DNA hybridized with the ss-DNA. The linear detectionrange was from 1.0 x 10(-13) M to 1.0 x 10(-7) M and the detection limit was 1.10 x 10(-14) M (n = 6). Compared with the other electrochemical DNA biosensors, the proposed biosensor showed its own performance of simplicity, good stability, and high sensitivity. (C) 2013 Elsevier B. V. All rights reserved.
dc.description.sponsorshipResearch Fellowship Programme for Foreign Citizens of the Scientific and Technological Research Council of Turkey (TUBITAK)
dc.description.sponsorshipThe authors thank Research Fellowship Programme for Foreign Citizens of the Scientific and Technological Research Council of Turkey (TUBITAK) for financial support.
dc.identifier.doi10.1016/j.snb.2013.08.034
dc.identifier.endpage1211
dc.identifier.issn0925-4005
dc.identifier.scopus2-s2.0-84883543418
dc.identifier.scopusqualityN/A
dc.identifier.startpage1201
dc.identifier.urihttps://doi.org/10.1016/j.snb.2013.08.034
dc.identifier.urihttps://hdl.handle.net/11486/6043
dc.identifier.volume188
dc.identifier.wosWOS:000326345600161
dc.identifier.wosqualityQ1
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherElsevier Science Sa
dc.relation.ispartofSensors and Actuators B-Chemical
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WOS_20250323
dc.subjectGraphene oxide
dc.subjectGold nanoparticles
dc.subjectElectrochemical impedance spectroscopy
dc.subjectDNA nanobiosensor
dc.titleA novel impedimetric biosensor based on graphene oxide/gold nanoplatform for detection of DNA arrays
dc.typeArticle

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