Antimicrobial magnetic poly(GMA) microparticles: synthesis, characterization and lysozyme immobilization

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dc.authorid, kadir/0000-0001-9158-6091
dc.authoridTATAR, Demet/0000-0002-9317-3263
dc.authoridVeyisoglu, Aysel/0000-0002-1406-5513
dc.contributor.authorErol, Kadir
dc.contributor.authorTatar, Demet
dc.contributor.authorVeyisoglu, Aysel
dc.contributor.authorTokatli, Ali
dc.date.accessioned2025-03-23T19:30:13Z
dc.date.available2025-03-23T19:30:13Z
dc.date.issued2021
dc.departmentSinop Üniversitesi
dc.description.abstractMicron-sized magnetic particles currently find a wide range of applications in many areas including biotechnology, biochemistry, colloid sciences and medicine. In this study, magnetic poly(glycidyl methacrylate) microparticles were synthesized by providing a polymerization around Fe(II)-Ni(II) magnetic double salt. Adsorption of lysozyme protein from aqueous systems was studied with these particles. Adsorption studies were performed with changing pH values, variable amount of adsorbent, different interaction times and lysozyme amounts. The adsorption capacity of the particles was investigated, and a value of about 95.6 mg lysozyme/g microparticle was obtained. The enzyme activity of the immobilized lysozyme was examined and found to be more stable and reusable compared to the free enzyme. The immobilized enzyme still showed 80% activity after five runs and managed to maintain 78% of its initial activity at the end of 60 days. Besides, in the antimicrobial analysis study for six different microorganisms, the minimum inhibitory concentration value of lysozyme immobilized particles was calculated as 125 tg/mL like free lysozyme. Finally, the adsorption interaction was found to be compatible with the Langmuir isotherm model. Accordingly, it can be said that magnetic poly(GMA) microparticles are suitable materials for lysozyme immobilization and immobilized lysozyme can be used in biotechnological studies.
dc.identifier.doi10.1515/polyeng-2020-0191
dc.identifier.endpage154
dc.identifier.issn0334-6447
dc.identifier.issn2191-0340
dc.identifier.issue2
dc.identifier.scopus2-s2.0-85098848409
dc.identifier.scopusqualityQ3
dc.identifier.startpage144
dc.identifier.urihttps://doi.org/10.1515/polyeng-2020-0191
dc.identifier.urihttps://hdl.handle.net/11486/5044
dc.identifier.volume41
dc.identifier.wosWOS:000626330500007
dc.identifier.wosqualityQ3
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherWalter De Gruyter Gmbh
dc.relation.ispartofJournal of Polymer Engineering
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WOS_20250323
dc.subjectactivity
dc.subjectadsorption
dc.subjectenzyme immobilization
dc.subjectlysozyme
dc.subjectmagnetic microparticle
dc.titleAntimicrobial magnetic poly(GMA) microparticles: synthesis, characterization and lysozyme immobilization
dc.typeArticle

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