ANALYSES OF parC AND gyrA MUTATIONS IN CIPROFLOXACIN-RESISTANT AND SUSCEPTIBLE pseudomonas aeruginosa ISOLATED FROM SOIL BY PCR-RFLP AND SSCP METHOD

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dc.authoridAvsar, cumhur/0000-0002-4095-0022
dc.contributor.authorAvsar, Cumhur
dc.contributor.authorAras, E. Sumer
dc.date.accessioned2025-03-23T19:30:08Z
dc.date.available2025-03-23T19:30:08Z
dc.date.issued2018
dc.departmentSinop Üniversitesi
dc.description.abstractThe aims of this study were to assess the prevalence of gyrA and parC mutations in ciprofloxacin-resistant and susceptible Pseudomonas aeruginosa isolated from soil and to evaluate the suitability of the restriction fragment length polymorphism (RFLP) and single-strand conformation polymorphism (SSCP) techniques as screening methods for molecular characterizations of ciprofloxacin resistance. From the examined 21 P. aeruginosa isolates 9 strains were resistant to ciprofloxacin. These 21 P. aeruginosa isolates and one control strain were analyzed for alterations in the ciprofloxacin resistance determining region of gyrA and parC by RFLP and SSCP analyses. The PCR reaction confirmed the presence of the gyrA and parC genes in all of the strains. PCR-RFLP analyses with SacII for gyrA and Hinfl for parC were performed as a screening method. We found that 18 and 17 out of 22 isolates have SacII and Hinfl restriction site and 4 and 5 strains did not have the site recognized by these enzymes, respectively. Seven for gyrA and fourteen for parC PCR products were electrophoresed for SSCP. By SSCP analysis, 21 (in parC) and 15 (in gyrA) different band patterns were detected, and each pattern corresponded to a distinct mutation. As a result, the RFLP and SSCP methods are suitable for a molecular screening of ciprofloxacin resistant and susceptible P. aeruginosa isolates. SSCP analysis can also provide advantage for the detection of novel and multiple mutations. In addition, we can say that environmental monitoring followed by clinical surveillance can be successful in uncovering previously unrecognized cases of infection.
dc.identifier.doi10.15414/jmbfs.2018.7.5.495-498
dc.identifier.endpage498
dc.identifier.issn1338-5178
dc.identifier.issue5
dc.identifier.scopus2-s2.0-85045082781
dc.identifier.scopusqualityN/A
dc.identifier.startpage495
dc.identifier.urihttps://doi.org/10.15414/jmbfs.2018.7.5.495-498
dc.identifier.urihttps://hdl.handle.net/11486/5024
dc.identifier.volume7
dc.identifier.wosWOS:000455216600010
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.indekslendigikaynakScopus
dc.language.isoen
dc.publisherSlovak Univ Agriculture Nitra
dc.relation.ispartofJournal of Microbiology Biotechnology and Food Sciences
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WOS_20250323
dc.subjectgyrA
dc.subjectparC
dc.subjectPCR-RFLP
dc.subjectPseudomonas aeruginosa
dc.subjectSSCP
dc.titleANALYSES OF parC AND gyrA MUTATIONS IN CIPROFLOXACIN-RESISTANT AND SUSCEPTIBLE pseudomonas aeruginosa ISOLATED FROM SOIL BY PCR-RFLP AND SSCP METHOD
dc.typeArticle

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