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Öğe Actinobacteria with heavy metal tolerance from the Sinop coast: A molecular characterization approach(Ege Univ, Fac Fisheries, 2025) Veyisoglu, Aysel; Tatar, Demet; Tokatli, Ali; Duyar, Hunkar AvniThis study aimed to isolate and molecularly characterize marine actinobacteria tolerant to heavy metals from sediment samples collected from the Meydankap & imath; coast in Sinop, T & uuml;rkiye. Sediment samples were obtained from three different stations and cultured on five selective media, leading to the isolation of 54 actinobacteria strains. The highest isolation efficiency was achieved on R2A and SM3 media. The tolerance of the isolates to heavy metals, including lead (Pb), copper (Cu), zinc (Zn), mercury (Hg), and cadmium (Cd), was tested. The results revealed that all isolates were sensitive to Hg and Cd but exhibited high tolerance to Pb. Twelve isolates, showing the highest resistance to heavy metals, were selected for genomic DNA isolation. The 16S rRNA gene region was amplified using universal primers and sequenced. Sequence analysis identified the isolates as belonging to the genera Streptomyces, Actinomadura, Nocardia and Nonomuraea. The approximately 1500-bp 16S rRNA gene sequences were compared with the EzTaxon database, and the closest species were determined. The obtained sequences were deposited in the GenBank database. The results indicate that marine actinobacteria isolated from polluted sediments of the Black Sea possess significant potential for bioremediation and environmental biotechnology applications due to their resistance mechanisms to heavy metals.Öğe Antimicrobial activity of a novel Streptomyces sp. K430 isolated from Northern Cyprus soil.(XVII. International Symposium on the Biology of Actinomycetes & Applications and Biotechnology of Actinomycetes., 2014) Saygın, Hayrettin; Veyisoğlu, Aysel; Tatar, Demet; Nigiz, Cengiz; Şahin, Nevzat[Abstract Not Available]Öğe Antimicrobial magnetic poly(GMA) microparticles: synthesis, characterization and lysozyme immobilization(Walter De Gruyter Gmbh, 2021) Erol, Kadir; Tatar, Demet; Veyisoglu, Aysel; Tokatli, AliMicron-sized magnetic particles currently find a wide range of applications in many areas including biotechnology, biochemistry, colloid sciences and medicine. In this study, magnetic poly(glycidyl methacrylate) microparticles were synthesized by providing a polymerization around Fe(II)-Ni(II) magnetic double salt. Adsorption of lysozyme protein from aqueous systems was studied with these particles. Adsorption studies were performed with changing pH values, variable amount of adsorbent, different interaction times and lysozyme amounts. The adsorption capacity of the particles was investigated, and a value of about 95.6 mg lysozyme/g microparticle was obtained. The enzyme activity of the immobilized lysozyme was examined and found to be more stable and reusable compared to the free enzyme. The immobilized enzyme still showed 80% activity after five runs and managed to maintain 78% of its initial activity at the end of 60 days. Besides, in the antimicrobial analysis study for six different microorganisms, the minimum inhibitory concentration value of lysozyme immobilized particles was calculated as 125 tg/mL like free lysozyme. Finally, the adsorption interaction was found to be compatible with the Langmuir isotherm model. Accordingly, it can be said that magnetic poly(GMA) microparticles are suitable materials for lysozyme immobilization and immobilized lysozyme can be used in biotechnological studies.Öğe Çorum Çevresindeki Yayla Topraklarından İzole Edilen Actinobacteria Üyelerinin Biyosentetik Genlerinin Taranması(2021) Tatar, Demet; Veyisoğlu, AyselBu çalışmada, Çorum (Kargı ve Osmancık) çevresindeki yaylalardan izole edilen aktinomiset izolatlarınınpoliketit sentaz ve ribozomal olmayan peptit sentetaz biyosentetik sistemlerinin varlığı moleküler tekniklerlearaştırılmıştır. Toplamda 15 tane Actinobacteria üyesinin sekonder metabolit biyosentetik genlerinin (PKS III/NRPS) varlığı PCR temelli incelenmiştir. Yapılan çalışmanın sonuçlarına göre, 15 tane aktinomiset izolatının 7tanesi PKS-I, 11 tanesi PKS-II ve 14 tanesinin NRPS gen bölgesine sahip olduğu bulunmuştur. Sonuçlardeğerlendirildiğinde izolatların nonribozomal peptid sentetaz (NRPS) gen kümelerini tip I-II poliketit sentazlar(PKS-I-II) gen kümelerinden daha fazla içerdiği görülmüştür. Ayrıca 5 izolatın üç gen kümesini bir aradabulundurduğu tespit edilmiştir. Sonuç olarak yaylalardan izole edilen aktinomisetlerin yüksek sekonder metabolitüretme kapasitesine sahip olduğu görülmüştür.Öğe Çorum İli Çevresinde Bulunan Tıbbi Öneme Sahip Bazı Bitkilerin Metanol Ekstraktlarının Antimikrobiyal Aktivitelerinin Belirlenmesi(2025) Tatar, Demet; Duyar, Hünkar Avni; Veyisoglu, Aysel; Kuruçay, NerminAmaç: Bu çalışmada, Çorum ilinde geleneksel tedavi amacıyla kullanılan on tıbbi bitkinin metanol ekstraktlarının antimikrobiyal aktivitelerinin araştırılması amaçlanmıştır. Materyal ve Yöntem: Bitki örnekleri ilkbahar döneminde toplanmış, kurutulduktan sonra %70 metanol ile ekstraksiyon yapılmıştır. Elde edilen ekstraktlar disk difüzyon yöntemi ile üç Gram-pozitif bakteri (Bacillus subtilis, Staphylococcus aureus, Enterococcus faecalis), üç Gram-negatif bakteri (Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa) ve iki maya-küf türü (Candida albicans, Aspergillus niger) üzerinde test edilmiştir. Pozitif kontroller olarak Ampisilin, Polimiksin B ve Nistatin kullanılmıştır. Araştırma Bulguları: Sonuçlar, dokuz bitki ekstraktının K. pneumoniae üzerinde, tüm ekstraktların ise B. subtilis üzerinde farklı düzeylerde inhibisyon gösterdiğini ortaya koymuştur. Yedi ekstrakt E. faecalis üzerinde etkili olurken, yalnızca meşe palamudu ve iğde ekstraktları A. niger üzerinde sınırlı antifungal aktivite göstermiştir. Buna karşın, S. aureus, E. coli, P. aeruginosa ve C. albicans üzerinde herhangi bir antimikrobiyal etki gözlenmemiştir. Sonuç: Çalışma, Çorum yöresinde kullanılan bazı bitkilerin antimikrobiyal potansiyel taşıdığını göstermektedir. Özellikle B. subtilis ve E. faecalis üzerinde etkili olan ekstraktlar, doğal ürünlerin yeni antimikrobiyal ajanlar geliştirilmesinde değerlendirilebileceğini işaret etmektedir. İlerleyen çalışmalarda bu bitkilerden elde edilen aktif bileşenlerin izolasyonu ve karakterizasyonu önerilmektedir.Öğe Detection of Bacterial Diversity of Various Habitats in Çorum Province and Its Criminalistics Contribution to Possible Crime Scene Studies(2024) Balci, Esra; Tatar, Demet; Veyisoglu, Aysel; Tokatlı, AliMicroorganisms are not homogeneously distributed in environments, soil systems are heterogeneous. Soil can be an important evidence value in forensic investigations. It is among the important evidences that contribute to the solution of forensic events in forensic sciences. Bacteria contained in the soil are microbiological evidences. Not all bacteria can be cultured by conventional methods and the amount of cultured bacteria remains limited. Metagenomic studies have been carried out for non-culturable Bacteria. The aim of this study is to perform DNA isolation from soil samples taken from Yeşil Lake (swamp), Faculty of Arts and Sciences garden, agricultural land, Sıklık (forest area) regions of Çorum Province in Türkiye and to determine bacterial diversity by metagenomic analysis of DNA isolated from soil samples. Density and differences of isolates according to habitats were determined. It is thought that the result of this study can shed light on previous crime scene studies in the determined habitats and will contribute to possible future crime scene studies and forensic science that may occur later.Öğe Determination of Amylolytic and Proteolytic Enzyme Activity Characteristics of Thermophilic Actinobacteria Isolated from Amasya- Terziköy Thermal Spring(2025) Tatar, Demet; Duyar, Hünkar Avni; Veyisoglu, Aysel; Yılmaz, Adem BarışActinobacteria represent a group of Gram-positive microorganisms characterized by high G+C content and are commonly found in both terrestrial and aquatic ecosystems. These bacteria are notable for their capacity to synthesize a wide range of bioactive compounds, including antibiotics and industrial enzymes. Their ability to withstand extreme environmental conditions allows them to colonize challenging habitats, such as thermal springs. These geothermal environments are critical not only for harboring microbial diversity but also for the discovery of novel bioactive substances with therapeutic potential. This study focused on isolating thermophilic actinobacteria from Terziköy Thermal Spring in Amasya, Türkiye, followed by phylogenetic analysis through 16S rRNA gene sequencing. The enzymatic capacities of the isolates—specifically amylase and protease production—were also assessed. Fourteen of the 21 isolates underwent 16S rRNA sequencing, resulting in the identification of eight Streptomyces, four Nocardia, one Actinomadura, and one Pseudonocardia species. Among these, seven isolates demonstrated amylase activity, and one displayed protease production. Notably, one Streptomyces isolate exhibited both enzymatic activities. These results highlight the phylogenetic diversity of thermophilic actinobacteria in geothermal environments and their potential as sources of thermostable enzymes for industrial applications.Öğe Determination of Amylolytic and Proteolytic Enzyme Activity Characteristics of Thermophilic Actinobacteria Isolated from Amasya-Terziköy Thermal Spring(Istanbul Univ Press, Fac Aquatic Sciences, 2025) Tatar, Demet; Veysioglu, Aysel; Tokatli, Ali; Duyar, Hunkar AvniActinobacteria represent a group of Gram-positive microorganisms characterized by high G+C content and are commonly found in both terrestrial and aquatic ecosystems. These bacteria are notable for their capacity to synthesize a wide range of bioactive compounds, including antibiotics and industrial enzymes. Their ability to withstand extreme environmental conditions allows them to colonize challenging habitats, such as thermal springs. These geothermal environments are critical not only for harboring microbial diversity but also for the discovery of novel bioactive substances with therapeutic potential. This study focused on isolating thermophilic actinobacteria from Terzik & ouml;y Thermal Spring in Amasya, T & uuml;rkiye, followed by phylogenetic analysis through 16S rRNA gene sequencing. The enzymatic capacities of the isolates-specifically amylase and protease production-were also assessed. Fourteen of the 21 isolates underwent 16S rRNA sequencing, resulting in the identification of eight Streptomyces, four Nocardia, one Actinomadura, and one Pseudonocardia species. Among these, seven isolates demonstrated amylase activity, and one displayed protease production. Notably, one Streptomyces isolate exhibited both enzymatic activities. These results highlightthe phylogenetic diversity ofthermophilic actinobacteria in geothermal environments and their potential as sources of thermostable enzymes for industrial applications.Öğe Determination of the Antimicrobial Potential of Naturally Grown Rosehip (Rosa canina L.) Fruit in the Giresun Region(2025) Tatar, Demet; Veyisoglu, AyselThe rise of antibiotic-resistant microorganisms has become a major global public health challenge, prompting the search for alternative antimicrobial agents. In this study, the antimicrobial activity of Rosa canina (rosehip) fruit extract, collected from the Alucra Plateau in Giresun, Türkiye, was evaluated under in vitro conditions. Methanolic extracts were tested against Gram-positive (Bacillus subtilis, Staphylococcus aureus) and Gram-negative (Escherichia coli, Enterobacter aerogenes) bacteria, as well as fungal strains (Candida albicans, Candida glabrata, Candida tropicalis) using the disk diffusion method. At a concentration of 30 mg/mL, the extract demonstrated significant inhibition zones against both bacterial and fungal pathogens. The most notable antifungal effects were observed against C. glabrata (20 mm) and C. tropicalis (16 mm). The absence of antimicrobial activity in the 25% DMSO control confirmed that the effects were solely attributable to the plant extract. These findings suggest that R. canina possesses promising natural antimicrobial properties and could serve as a complementary treatment option, particularly against multidrug-resistant pathogens.Öğe Diversity and antimicrobial activity of culturable actinobacteria isolated from the sediment of Sarikum Lake(Taylor & Francis Ltd, 2021) Veyisoglu, Aysel; Tatar, DemetIn this study, actinobacteria isolated from Sarikum Lake sediment were characterized (Sinop-Turkey). A total of 48 actinobacteria were isolated using ten selective media with the dilution-plating method. The phylogenetic analysis according to 16S rRNA gene sequencing showed that 40% of the isolated strains belong to the widely distributed genus of Streptomyces, 36% belong to genus of Micromonospora, 24% of the isolates belong to rare genera such as Rhodococcus, Plantactinospora, Nonomuraea, Actinomadura and Streptosporangium. Most of the isolated strains belong to the genus Streptomyces (40%) and two isolates may be new species. All of the isolates were tested for antimicrobial activity; only 12 isolates exhibited antimicrobial activity. Nevertheless, 11 isolates were active against gram-positive, 5 were potential against gram-negative and no isolates had any effect against pathogenic fungi. All of the 48 isolates were analysed for genes encoding nonribosomal peptide synthetases (NRPS) and polyketide synthases (PKS). In all of the 48 isolates were detected NRPS sequences, PKS-I in 28 isolates and PKS-II in 22 isolates. PKS-I - PKS-II - NRPS genes were identified in 17 isolates.Öğe Enhanced kinetic performance and stability of catalase immobilized on epoxy-functionalized kaolinite(Nature Portfolio, 2026) Erol, Kadir; Veyisoglu, Aysel; Tatar, Demet; Kocabas, Buket Bulut; Alacabey, Ihsan; Gokmese, EbruThe immobilization of catalase onto stable, reusable supports is crucial for efficient peroxide-based biocatalytic applications. In this study, catalase was immobilized for the first time onto epoxy-functionalized kaolinite particles prepared via surface silanization with (3-glycidyloxypropyl)trimethoxysilane. Structural and surface characterizations confirmed successful organosilane grafting while preserving the layered kaolinite framework. The modified support exhibited rapid enzyme uptake and a high immobilization capacity of approximately 300 mg g(-1). Kinetic analysis showed a substantial decrease in K-m from 57.3 mM (free catalase) to 21.6 mM after immobilization, indicating enhanced substrate affinity. In contrast, V-max decreased due to diffusion limitations typical of heterogeneous systems. Despite this, catalytic efficiency increased nearly 1.8-fold. Moreover, immobilized catalase demonstrated significantly improved operational reusability and long-term storage stability compared to the free enzyme. These results highlight silanized kaolinite as a robust, low-cost, and efficient mineral-based support for catalase immobilization, with strong potential for environmental and industrial biocatalytic applications.Öğe Investigation of the antimicrobial activity of water and methanol extracts of Salvadora persica L. (Miswak) plant against some pathogenic microorganisms(Gültekin ÖZDEMIR, 2024) Veyisoglu, Aysel; Balta, Yusuf Emre; Tatar, DemetThe toothbrush tree Salvadora persica L, also called miswak, belonging to the family Salvadoraceae, is one of the most important of the 182 plant species used as chewing sticks. It is widely used in many Asian, African and Middle Eastern countries. The roots, branches and stems of this plant have been used for oral hygiene and small miswak sticks have been used as toothpicks for oral hygiene. In this study, commercially purchased Salvadora persica, L. (Miswak) plant used in oral hygiene were tested against seven pathogenic bacteria (Bacillus cereus ATCC 10987, Bacillus subtilis ATCC 6623, Staphylococcus aureus ATCC 25923, Enterococcus faecalis ATCC 29212, Escherichia coli ATCC 25922, Klebsiella pneumoniae ATCC 70060, Pseudomonas aeruginosa ATCC 27853) and two fungi (Candida albicans ATCC 10231 and Aspergillus niger ATCC 16404) at eight different concentrations (200 mg/ml, 100 mg/ml, 50 mg/ml, 25 mg/ml, 12. 5 mg/ml, 6 mg/ml, 3 mg/ml and 1 mg/ml) were determined. While the aqueous extract did not show any antimicrobial activity against seven pathogens, the methanol extract showed activity against three pathogens. The methanol extract of S. persica showed antimicrobial activity against Bacillus cereus ATCC 10987, Bacillus subtilis ATCC 6623 and Klebsiella pneumoniae ATCC 70060 strains.Öğe Investigation of the Antimicrobial Effects of Traditionally Prepared Vinegars from Plantago major L. and Hypericum perforatum L. Collected in Ordu Province on Selected Pathogenic Microorganisms(2025) Tatar, Demet; Veyisoglu, Aysel; Tepeköy, İremIn this study, the antimicrobial effects of traditionally fermented vinegars produced from Plantago major L. (common plantain) and Hypericum perforatum L. (St. John’s Wort), collected from the Ünye district of Ordu Province, Türkiye, were evaluated. The vinegar samples were tested against 11 pathogenic microorganisms, including Gram-positive and Gram-negative bacteria as well as Candida species, using the agar disk diffusion method. The results showed that both types of vinegar exhibited significant antimicrobial activity, particularly at undiluted (1:1) and 1/2 dilutions. The highest antibacterial effect for P. major vinegar was observed against Proteus vulgaris (15.33±0.33 mm). Antifungal activity, however, was generally lower compared to bacterial inhibition. The pH values of P. major and H. perforatum vinegars were measured as 2.62 and 2.72, respectively, indicating strong acidity, which likely contributes to their antimicrobial properties. These findings suggest that vinegars produced from medicinal plants through traditional fermentation methods have the potential to serve as natural antimicrobial agents. Further biochemical studies are recommended to explore their potential applications in pharmaceutical and food safety fields.Öğe Isolation, Molecular Characterization and Determination of Antagonistic Properties of Alkalitolerant Streptomyces Members from Van Lake-Çarpanak Island Soil(2024) Veyisoglu, Aysel; Tatar, Demet; Duyar, Hünkar Avni; Tokatlı, Ali; Tokatlı, AliÇarpanak Island is one of the islands in Lake Van, the easternmost and largest lake in Türkiye. In this study, soil samples taken from three different locations of Çarpanak island, Lake Van, were inoculated into 4 different selective isolation media using the dilution plate technique, and 42 actinomycete isolates were stocked. PCR amplification of the 16S rRNA gene of 13 isolates selected according to their morphological differences was performed with 27F and 1525R primers. Phylogenetic trees were constructed with the neighbor-joining algorithm in the MEGA 7.0 software. According to 16S rRNA gene sequence analysis, it was determined that 13 isolates belonged to the genus Streptomyces. The antimicrobial activities of 13 isolates against 8 pathogens and the ability of these isolates to produce lipase, amylase, protease and pectinase were determined. Except for CA43 and CA62 isolates, other Streptomyces isolates have the ability to produce lipase. Other Streptomyces isolates except CA59 and CA94 can synthesize amylase. In addition, 3 of the 13 Streptomyces isolates, CA40, CA61 and CA94, do not have the ability to synthesize protease. No isolate is capable of producing pectinase enzyme. As a result of this study, it was observed that most of the Streptomyces isolates had higher lipase enzyme production abilities than other enzyme groups.Öğe Kargı ve Osmancık Çevresindeki Yayla Topraklarının Aktinomiset Çeşitliliği(2020) Tatar, Demet; Veyisoglu, AyselBu çalışmada Çorum iline bağlı Kargı (Eğinönü), Abdullah ve Osmancık-Başpınar Karaca yaylalarından alınan toprak örneklerinden izole edilen aktinomiset suşlarının 16S rRNA gen bölgesi analizlerine göre aktinomiset çeşitliliğinin saptanması amaçlandı. Dilüsyon plak tekniğinin uygulandığı izolasyon çalışmasında 5 farklı seçici besiyeri kullanıldı. İzolatların beş farklı kültür ortamında kültürel karakterizasyonları yapıldı. Test izolatlarının 16S rRNA gen bölgesinin PZR amplifikasyonu 27F ve 1525R primerleri ile gerçekleştirildi. Filogenetik ağaçlar,MEGA 7.0 yazılım programında neighbor-joining algoritmasıyla oluşturuldu. 5 farklı izolasyon besiyerinden toplam 15 mikroorganizma seçildi ve 16S rRNA gen dizi analizine göre 15 izolatın, altısı Nocardia cinsi üyesi, beşi Streptomyces cinsi üyesi, biri Haloactinopolyspora cinsi üyesi, biri Micromonospora cinsi üyesi, biriNocardiopsis cinsi üyesi ve birinin de Rhodococcus cinsi üyesi olduğu tespit edildi.Öğe Microvirga makkahensis sp nov., and Microvirga arabica sp nov., isolated from sandy arid soil(Springer, 2016) Veyisoglu, Aysel; Tatar, Demet; Saygin, Hayrettin; Inan, Kadriye; Cetin, Demet; Guven, Kiymet; Tuncer, MunirThe taxonomic positions of two Gram-negative strains, SV1470(T) and SV2184P(T), isolated from arid soil samples, were determined using a polyphasic approach. Analysis of the 16S rRNA gene and the concatenated sequences of three housekeeping gene loci (dnaK, rpoB and gyrB) confirmed that the strains belong to the genus Microvirga. Strain SV1470(T) was found to be closely related to Microvirga vignae BR3299(T) (98.8 %), Microvirga flocculans TFBT (98.3 %) and Microvirga lupini Lut6(T) (98.2 %), whilst similarity to other type strains of the genus ranged from 97.8 to 96.3 %; strain SV2184P(T) was found to be closely related to Microvirga aerilata 5420S-16(T) (98.0 %), Microvirga zambiensis WSM3693(T) (97.8 %) and M. flocculans ATCC BAA-817(T) (97.4 %), whilst similarity to other type strains of the genus ranged from 97.2 to 95.9 %. The G + C content of the genomic DNA was determined to be 61.5 mol % for strain SV1470(T) and 62.1 mol % for strain SV2184P(T). Both strains were found to have the same quinone system, with Q-10 as the major ubiquinone. The polar lipid profile of strain SV1470(T) was found to consist of phosphatidylcholine, phosphatidylglycerol, phosphatidylethanolamine, one unidentified phospholipid and one unidentified aminolipid, while that of strain SV2184P(T) consisted of phosphatidylcholine, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylmethylethanolamine, one unidentified aminolipid, one unidentified aminophospholipid and two unidentified phospholipids. DNA-DNA relatedness studies showed that the two strains belong to different genomic species. The strains were also distinguished using a combination of phenotypic properties. Based on the genotypic and phenotypic data, the novel species Microvirga makkahensis sp. nov. (type strain SV1470(T) = DSM 25394(T) = KCTC 23863(T) = NRRL-B 24875(T)) and Microvirga arabica sp. nov. (type strain SV2184P(T) = DSM 25393(T) = KCTC 23864(T) = NRRL-B 24874(T)) are proposed.Öğe Nijerya Toprağından Yeni Bir Lechevalieria Türünün Polifazik Yöntemlerle Karakterizasyonu(21. Ulusal Biyoloji Kongresi, 03–07 Eylül 2012, Ege Üniversitesi, İzmir, Türkiye, 2012) Çamaş, Mustafa; Veyisoğlu, Aysel; Tatar, Demet; Saygın, Hayrettin; Sazak, Anıl; Şahin, Nevzat[Abstract Not Available]Öğe Phytomonospora cypria sp nov., isolated from soil(Springer, 2015) Sahin, Nevzat; Veyisoglu, Aysel; Tatar, Demet; Saygin, Hayrettin; Cetin, Demet; Guven, Kiymet; Klenk, Hans-PeterA Gram-stain positive actinobacterial strain, designated KT1403(T), was isolated from a soil sample, collected from Karpaz, Magusa, Northern Cyprus, and characterised using a polyphasic approach. Morphological characteristics and chemotaxonomic data indicated that the strain belongs to the genus Phytomonospora. The cell wall of the novel strain contained meso-diaminopimelic acid and galactose, glucose and mannose as the major sugars in whole cell hydrolysates. The polar lipids in the cell membrane were identified as diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, an unidentified aminophospholipid and three unidentified glycolipids. The predominant menaquinones were MK-10(H-6) and MK-10(H-4). The major fatty acids were found to be iso C-15:0 , anteiso C-15:0 and anteiso C-17:0. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain KT1403(T) belongs to the genus Phytomonospora with a sequence similarity of 99.73 % with Phytomonospora endophytica, the type species of the genus. DNA-DNA hybridization further differentiated strain KT1403(T) from its near phylogenetic neighbour, P. endophytica DSM 45386(T) (29.0 +/- A 2.2 % DNA relatedness). Therefore, it is proposed that strain KT1403(T) represents a novel species of the genus Phytomonospora, for which the name Phytomonospora cypria sp. nov. is proposed. The type strain is KT1403(T) (=KCTC 29479(T) = DSM 46767(T)).Öğe Polyphasic characterization of a novel Streptomyces sp. K427 isolated from arid soil.(XVII. International Symposium on the Biology of Actinomycetes & Applications and Biotechnology of Actinomycetes., 2014) Veyisoğlu, Aysel; Tatar, Demet; Saygın, Hayrettin; Nigiz, Cengiz; Şahin, Nevzat[Abstract Not Available]Öğe Saccharopolyspora hattusasensis sp nov., isolated from soil(Springer, 2017) Veyisoglu, Aysel; Saygin, Hayrettin; Tatar, Demet; Bektas, Kadriye Inan; Sahin, NevzatA Saccharopolyspora strain, designated CR3506(T), isolated from a soil sample collected from Sungurlu, Corum, Turkey, was examinated using a polyphasic approach. Phylogenetic analysis based on an almost-complete 16S rRNA gene sequence analysis showed that the strain is closely related to the type strains of Saccharopolyspora spinosa NRRL 18395(T) (99.1%), Saccharopolyspora phatthalungensis NRRL B-24798(T) (98.4%) and Saccharopolyspora shandongensis 88(T) (98.1%); low levels of DNA-DNA relatedness were found between the isolate and S. spinosa and S. phatthalungensis (< 50%). Strain CR3506(T) was found to have chemotaxonomic and phylogenetic properties consistent with its classification in the genus Saccharopolyspora. The strain contained meso-diaminopimelic acid as the diagnostic diamino acid. Whole-cell hydrolysates contained arabinose and galactose. The polar lipids were identified as phosphatidylmethylethanolamine, phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylcholine, phosphatidylglycerol and phosphatidylinositol. The predominant menaquinones (> 10%) were MK-9(H-4) and MK-8(H-4). Major fatty acids were (> 10%) iso-C-16:0, C(15:0)3OH, C-18:0 and iso-C-15:0. Further, the morphological, physiological and biochemical characteristics of strain CR3506(T) are distinct from S. spinosa and other species of the genus Saccharopolyspora with which this strain has high 16S rRNA gene sequence similarity (98.0-98.5%). Strain CR3506(T) has antimicrobial activity against Bacillus subtilis NRRL B-209, Citrobacter freundi NRRL B-2643 and Staphylococcus aureus ATCC 29213. Consequently, it is proposed that strain CR3506(T) represents a novel Saccharopolyspora species for which the name Saccharopolyspora hattusasensis sp. nov. is proposed. The type strain is CR3506(T) (=KCTC 29104(T) = DSM 45715(T)).
