DSpace Arşivi :: by Yazar "Tatar, Demet" değerine göre listeleniyor

Yazar "Tatar, Demet" seçeneğine göre listele

Listeleniyor 1 - 20 / 20

Antimicrobial activity of a novel Streptomyces sp. K430 isolated from Northern Cyprus soil.
(XVII. International Symposium on the Biology of Actinomycetes & Applications and Biotechnology of Actinomycetes., 2014) Saygın, Hayrettin; Veyisoğlu, Aysel; Tatar, Demet; Nigiz, Cengiz; Şahin, Nevzat
[Abstract Not Available]
Antimicrobial magnetic poly(GMA) microparticles: synthesis, characterization and lysozyme immobilization
(Walter De Gruyter Gmbh, 2021) Erol, Kadir; Tatar, Demet; Veyisoglu, Aysel; Tokatli, Ali
Micron-sized magnetic particles currently find a wide range of applications in many areas including biotechnology, biochemistry, colloid sciences and medicine. In this study, magnetic poly(glycidyl methacrylate) microparticles were synthesized by providing a polymerization around Fe(II)-Ni(II) magnetic double salt. Adsorption of lysozyme protein from aqueous systems was studied with these particles. Adsorption studies were performed with changing pH values, variable amount of adsorbent, different interaction times and lysozyme amounts. The adsorption capacity of the particles was investigated, and a value of about 95.6 mg lysozyme/g microparticle was obtained. The enzyme activity of the immobilized lysozyme was examined and found to be more stable and reusable compared to the free enzyme. The immobilized enzyme still showed 80% activity after five runs and managed to maintain 78% of its initial activity at the end of 60 days. Besides, in the antimicrobial analysis study for six different microorganisms, the minimum inhibitory concentration value of lysozyme immobilized particles was calculated as 125 tg/mL like free lysozyme. Finally, the adsorption interaction was found to be compatible with the Langmuir isotherm model. Accordingly, it can be said that magnetic poly(GMA) microparticles are suitable materials for lysozyme immobilization and immobilized lysozyme can be used in biotechnological studies.
Çorum Çevresindeki Yayla Topraklarından İzole Edilen Actinobacteria Üyelerinin Biyosentetik Genlerinin Taranması
(2021) Tatar, Demet; Veyisoğlu, Aysel
Bu çalışmada, Çorum (Kargı ve Osmancık) çevresindeki yaylalardan izole edilen aktinomiset izolatlarınınpoliketit sentaz ve ribozomal olmayan peptit sentetaz biyosentetik sistemlerinin varlığı moleküler tekniklerlearaştırılmıştır. Toplamda 15 tane Actinobacteria üyesinin sekonder metabolit biyosentetik genlerinin (PKS III/NRPS) varlığı PCR temelli incelenmiştir. Yapılan çalışmanın sonuçlarına göre, 15 tane aktinomiset izolatının 7tanesi PKS-I, 11 tanesi PKS-II ve 14 tanesinin NRPS gen bölgesine sahip olduğu bulunmuştur. Sonuçlardeğerlendirildiğinde izolatların nonribozomal peptid sentetaz (NRPS) gen kümelerini tip I-II poliketit sentazlar(PKS-I-II) gen kümelerinden daha fazla içerdiği görülmüştür. Ayrıca 5 izolatın üç gen kümesini bir aradabulundurduğu tespit edilmiştir. Sonuç olarak yaylalardan izole edilen aktinomisetlerin yüksek sekonder metabolitüretme kapasitesine sahip olduğu görülmüştür.
Detection of Bacterial Diversity of Various Habitats in Çorum Province and Its Criminalistics Contribution to Possible Crime Scene Studies
(2024) Balci, Esra; Tatar, Demet; Veyisoglu, Aysel; Tokatlı, Ali
Microorganisms are not homogeneously distributed in environments, soil systems are heterogeneous. Soil can be an important evidence value in forensic investigations. It is among the important evidences that contribute to the solution of forensic events in forensic sciences. Bacteria contained in the soil are microbiological evidences. Not all bacteria can be cultured by conventional methods and the amount of cultured bacteria remains limited. Metagenomic studies have been carried out for non-culturable Bacteria. The aim of this study is to perform DNA isolation from soil samples taken from Yeşil Lake (swamp), Faculty of Arts and Sciences garden, agricultural land, Sıklık (forest area) regions of Çorum Province in Türkiye and to determine bacterial diversity by metagenomic analysis of DNA isolated from soil samples. Density and differences of isolates according to habitats were determined. It is thought that the result of this study can shed light on previous crime scene studies in the determined habitats and will contribute to possible future crime scene studies and forensic science that may occur later.
Diversity and antimicrobial activity of culturable actinobacteria isolated from the sediment of Sarikum Lake
(Taylor & Francis Ltd, 2021) Veyisoglu, Aysel; Tatar, Demet
In this study, actinobacteria isolated from Sarikum Lake sediment were characterized (Sinop-Turkey). A total of 48 actinobacteria were isolated using ten selective media with the dilution-plating method. The phylogenetic analysis according to 16S rRNA gene sequencing showed that 40% of the isolated strains belong to the widely distributed genus of Streptomyces, 36% belong to genus of Micromonospora, 24% of the isolates belong to rare genera such as Rhodococcus, Plantactinospora, Nonomuraea, Actinomadura and Streptosporangium. Most of the isolated strains belong to the genus Streptomyces (40%) and two isolates may be new species. All of the isolates were tested for antimicrobial activity; only 12 isolates exhibited antimicrobial activity. Nevertheless, 11 isolates were active against gram-positive, 5 were potential against gram-negative and no isolates had any effect against pathogenic fungi. All of the 48 isolates were analysed for genes encoding nonribosomal peptide synthetases (NRPS) and polyketide synthases (PKS). In all of the 48 isolates were detected NRPS sequences, PKS-I in 28 isolates and PKS-II in 22 isolates. PKS-I - PKS-II - NRPS genes were identified in 17 isolates.
Investigation of the antimicrobial activity of water and methanol extracts of Salvadora persica L. (Miswak) plant against some pathogenic microorganisms
(Gültekin ÖZDEMIR, 2024) Veyisoglu, Aysel; Balta, Yusuf Emre; Tatar, Demet
The toothbrush tree Salvadora persica L, also called miswak, belonging to the family Salvadoraceae, is one of the most important of the 182 plant species used as chewing sticks. It is widely used in many Asian, African and Middle Eastern countries. The roots, branches and stems of this plant have been used for oral hygiene and small miswak sticks have been used as toothpicks for oral hygiene. In this study, commercially purchased Salvadora persica, L. (Miswak) plant used in oral hygiene were tested against seven pathogenic bacteria (Bacillus cereus ATCC 10987, Bacillus subtilis ATCC 6623, Staphylococcus aureus ATCC 25923, Enterococcus faecalis ATCC 29212, Escherichia coli ATCC 25922, Klebsiella pneumoniae ATCC 70060, Pseudomonas aeruginosa ATCC 27853) and two fungi (Candida albicans ATCC 10231 and Aspergillus niger ATCC 16404) at eight different concentrations (200 mg/ml, 100 mg/ml, 50 mg/ml, 25 mg/ml, 12. 5 mg/ml, 6 mg/ml, 3 mg/ml and 1 mg/ml) were determined. While the aqueous extract did not show any antimicrobial activity against seven pathogens, the methanol extract showed activity against three pathogens. The methanol extract of S. persica showed antimicrobial activity against Bacillus cereus ATCC 10987, Bacillus subtilis ATCC 6623 and Klebsiella pneumoniae ATCC 70060 strains.
Isolation, Molecular Characterization and Determination of Antagonistic Properties of Alkalitolerant Streptomyces Members from Van Lake-Çarpanak Island Soil
(2024) Veyisoglu, Aysel; Tatar, Demet; Duyar, Hünkar Avni; Tokatlı, Ali; Tokatlı, Ali
Çarpanak Island is one of the islands in Lake Van, the easternmost and largest lake in Türkiye. In this study, soil samples taken from three different locations of Çarpanak island, Lake Van, were inoculated into 4 different selective isolation media using the dilution plate technique, and 42 actinomycete isolates were stocked. PCR amplification of the 16S rRNA gene of 13 isolates selected according to their morphological differences was performed with 27F and 1525R primers. Phylogenetic trees were constructed with the neighbor-joining algorithm in the MEGA 7.0 software. According to 16S rRNA gene sequence analysis, it was determined that 13 isolates belonged to the genus Streptomyces. The antimicrobial activities of 13 isolates against 8 pathogens and the ability of these isolates to produce lipase, amylase, protease and pectinase were determined. Except for CA43 and CA62 isolates, other Streptomyces isolates have the ability to produce lipase. Other Streptomyces isolates except CA59 and CA94 can synthesize amylase. In addition, 3 of the 13 Streptomyces isolates, CA40, CA61 and CA94, do not have the ability to synthesize protease. No isolate is capable of producing pectinase enzyme. As a result of this study, it was observed that most of the Streptomyces isolates had higher lipase enzyme production abilities than other enzyme groups.
Kargi ve Osmancik Çevresindeki Yayla Topraklarinin Aktinomiset Çesitliligi
(Bilecik Seyh Edebali Üniversitesi, 2020) Tatar, Demet; Veyisoglu, Aysel
Bu çalismada Çorum iline bagli Kargi (Eginönü), Abdullah ve Osmancik-Baspinar Karaca yaylalarindan alinan toprak örneklerinden izole edilen aktinomiset suslarinin 16S rRNA gen bölgesi analizlerine göre aktinomiset çesitliliginin saptanmasi amaçlandi. Dilüsyon plak tekniginin uygulandigi izolasyon çalismasinda 5 farkli seçici besiyeri kullanildi. Izolatlarin bes farkli kültür ortaminda kültürel karakterizasyonlari yapildi. Test izolatlarinin 16S rRNA gen bölgesinin PZR amplifikasyonu 27F ve 1525R primerleri ile gerçeklestirildi. Filogenetik agaçlar, MEGA 7.0 yazilim programinda neighbor-joining algoritmasiyla olusturuldu. 5 farkli izolasyon besiyerinden toplam 15 mikroorganizma seçildi ve 16S rRNA gen dizi analizine göre 15 izolatin, altisi Nocardia cinsi üyesi, besi Streptomyces cinsi üyesi, biri Haloactinopolyspora cinsi üyesi, biri Micromonospora cinsi üyesi, biri Nocardiopsis cinsi üyesi ve birinin de Rhodococcus cinsi üyesi oldugu tespit edildi.
Kargı ve Osmancık Çevresindeki Yayla Topraklarının Aktinomiset Çeşitliliği
(2020) Tatar, Demet; Veyisoglu, Aysel
Bu çalışmada Çorum iline bağlı Kargı (Eğinönü), Abdullah ve Osmancık-Başpınar Karaca yaylalarından alınan toprak örneklerinden izole edilen aktinomiset suşlarının 16S rRNA gen bölgesi analizlerine göre aktinomiset çeşitliliğinin saptanması amaçlandı. Dilüsyon plak tekniğinin uygulandığı izolasyon çalışmasında 5 farklı seçici besiyeri kullanıldı. İzolatların beş farklı kültür ortamında kültürel karakterizasyonları yapıldı. Test izolatlarının 16S rRNA gen bölgesinin PZR amplifikasyonu 27F ve 1525R primerleri ile gerçekleştirildi. Filogenetik ağaçlar,MEGA 7.0 yazılım programında neighbor-joining algoritmasıyla oluşturuldu. 5 farklı izolasyon besiyerinden toplam 15 mikroorganizma seçildi ve 16S rRNA gen dizi analizine göre 15 izolatın, altısı Nocardia cinsi üyesi, beşi Streptomyces cinsi üyesi, biri Haloactinopolyspora cinsi üyesi, biri Micromonospora cinsi üyesi, biriNocardiopsis cinsi üyesi ve birinin de Rhodococcus cinsi üyesi olduğu tespit edildi.
Microvirga makkahensis sp nov., and Microvirga arabica sp nov., isolated from sandy arid soil
(Springer, 2016) Veyisoglu, Aysel; Tatar, Demet; Saygin, Hayrettin; Inan, Kadriye; Cetin, Demet; Guven, Kiymet; Tuncer, Munir
The taxonomic positions of two Gram-negative strains, SV1470(T) and SV2184P(T), isolated from arid soil samples, were determined using a polyphasic approach. Analysis of the 16S rRNA gene and the concatenated sequences of three housekeeping gene loci (dnaK, rpoB and gyrB) confirmed that the strains belong to the genus Microvirga. Strain SV1470(T) was found to be closely related to Microvirga vignae BR3299(T) (98.8 %), Microvirga flocculans TFBT (98.3 %) and Microvirga lupini Lut6(T) (98.2 %), whilst similarity to other type strains of the genus ranged from 97.8 to 96.3 %; strain SV2184P(T) was found to be closely related to Microvirga aerilata 5420S-16(T) (98.0 %), Microvirga zambiensis WSM3693(T) (97.8 %) and M. flocculans ATCC BAA-817(T) (97.4 %), whilst similarity to other type strains of the genus ranged from 97.2 to 95.9 %. The G + C content of the genomic DNA was determined to be 61.5 mol % for strain SV1470(T) and 62.1 mol % for strain SV2184P(T). Both strains were found to have the same quinone system, with Q-10 as the major ubiquinone. The polar lipid profile of strain SV1470(T) was found to consist of phosphatidylcholine, phosphatidylglycerol, phosphatidylethanolamine, one unidentified phospholipid and one unidentified aminolipid, while that of strain SV2184P(T) consisted of phosphatidylcholine, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylmethylethanolamine, one unidentified aminolipid, one unidentified aminophospholipid and two unidentified phospholipids. DNA-DNA relatedness studies showed that the two strains belong to different genomic species. The strains were also distinguished using a combination of phenotypic properties. Based on the genotypic and phenotypic data, the novel species Microvirga makkahensis sp. nov. (type strain SV1470(T) = DSM 25394(T) = KCTC 23863(T) = NRRL-B 24875(T)) and Microvirga arabica sp. nov. (type strain SV2184P(T) = DSM 25393(T) = KCTC 23864(T) = NRRL-B 24874(T)) are proposed.
Nijerya Toprağından Yeni Bir Lechevalieria Türünün Polifazik Yöntemlerle Karakterizasyonu
(21. Ulusal Biyoloji Kongresi, 03–07 Eylül 2012, Ege Üniversitesi, İzmir, Türkiye, 2012) Çamaş, Mustafa; Veyisoğlu, Aysel; Tatar, Demet; Saygın, Hayrettin; Sazak, Anıl; Şahin, Nevzat
[Abstract Not Available]
Phytomonospora cypria sp nov., isolated from soil
(Springer, 2015) Sahin, Nevzat; Veyisoglu, Aysel; Tatar, Demet; Saygin, Hayrettin; Cetin, Demet; Guven, Kiymet; Klenk, Hans-Peter
A Gram-stain positive actinobacterial strain, designated KT1403(T), was isolated from a soil sample, collected from Karpaz, Magusa, Northern Cyprus, and characterised using a polyphasic approach. Morphological characteristics and chemotaxonomic data indicated that the strain belongs to the genus Phytomonospora. The cell wall of the novel strain contained meso-diaminopimelic acid and galactose, glucose and mannose as the major sugars in whole cell hydrolysates. The polar lipids in the cell membrane were identified as diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, an unidentified aminophospholipid and three unidentified glycolipids. The predominant menaquinones were MK-10(H-6) and MK-10(H-4). The major fatty acids were found to be iso C-15:0 , anteiso C-15:0 and anteiso C-17:0. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain KT1403(T) belongs to the genus Phytomonospora with a sequence similarity of 99.73 % with Phytomonospora endophytica, the type species of the genus. DNA-DNA hybridization further differentiated strain KT1403(T) from its near phylogenetic neighbour, P. endophytica DSM 45386(T) (29.0 +/- A 2.2 % DNA relatedness). Therefore, it is proposed that strain KT1403(T) represents a novel species of the genus Phytomonospora, for which the name Phytomonospora cypria sp. nov. is proposed. The type strain is KT1403(T) (=KCTC 29479(T) = DSM 46767(T)).
Polyphasic characterization of a novel Streptomyces sp. K427 isolated from arid soil.
(XVII. International Symposium on the Biology of Actinomycetes & Applications and Biotechnology of Actinomycetes., 2014) Veyisoğlu, Aysel; Tatar, Demet; Saygın, Hayrettin; Nigiz, Cengiz; Şahin, Nevzat
[Abstract Not Available]
Saccharopolyspora hattusasensis sp nov., isolated from soil
(Springer, 2017) Veyisoglu, Aysel; Saygin, Hayrettin; Tatar, Demet; Bektas, Kadriye Inan; Sahin, Nevzat
A Saccharopolyspora strain, designated CR3506(T), isolated from a soil sample collected from Sungurlu, Corum, Turkey, was examinated using a polyphasic approach. Phylogenetic analysis based on an almost-complete 16S rRNA gene sequence analysis showed that the strain is closely related to the type strains of Saccharopolyspora spinosa NRRL 18395(T) (99.1%), Saccharopolyspora phatthalungensis NRRL B-24798(T) (98.4%) and Saccharopolyspora shandongensis 88(T) (98.1%); low levels of DNA-DNA relatedness were found between the isolate and S. spinosa and S. phatthalungensis (< 50%). Strain CR3506(T) was found to have chemotaxonomic and phylogenetic properties consistent with its classification in the genus Saccharopolyspora. The strain contained meso-diaminopimelic acid as the diagnostic diamino acid. Whole-cell hydrolysates contained arabinose and galactose. The polar lipids were identified as phosphatidylmethylethanolamine, phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylcholine, phosphatidylglycerol and phosphatidylinositol. The predominant menaquinones (> 10%) were MK-9(H-4) and MK-8(H-4). Major fatty acids were (> 10%) iso-C-16:0, C(15:0)3OH, C-18:0 and iso-C-15:0. Further, the morphological, physiological and biochemical characteristics of strain CR3506(T) are distinct from S. spinosa and other species of the genus Saccharopolyspora with which this strain has high 16S rRNA gene sequence similarity (98.0-98.5%). Strain CR3506(T) has antimicrobial activity against Bacillus subtilis NRRL B-209, Citrobacter freundi NRRL B-2643 and Staphylococcus aureus ATCC 29213. Consequently, it is proposed that strain CR3506(T) represents a novel Saccharopolyspora species for which the name Saccharopolyspora hattusasensis sp. nov. is proposed. The type strain is CR3506(T) (=KCTC 29104(T) = DSM 45715(T)).
Saccharopolyspora soli sp. nov., isolated from Northern Cyprus soil
(Springer, 2022) Veyisoglu, Aysel; Tatar, Demet; Saygin, Hayrettin; Cetin, Demet; Guven, Kiymet; Sahin, Nevzat
A novel Gram-stain positive, aerobic, non-motile actinobacterium, designated strain K220(T), was isolated from soil collected from Cape Andreas (Zafer Burnu), Northern Cyprus, and subjected to a polyphasic taxonomic approach. The organism was shown to have phylogenetic, chemotaxonomic, cultural and morphological properties consistent with its classification in the genus Saccharopolyspora. 16S rRNA gene sequence analysis of strain K220(T) showed that it is closely related to the type strains of Saccharopolyspora maritima 3SS5-12 (T), Saccharopolyspora kobensis JCM 9109 (T) and Saccharopolyspora hirsuta ATCC 27875 (T) with 97.6, 97.5 and 97.0% sequence similarity, respectively. In silico DNA-DNA hybridization and average nucleotide identity values between strain K220(T) and type strains of the genus Saccharopolyspora with publicly available genomes were 22.1-31.2% and 76.0-83.16%, respectively. The DNA G + C content of strain K220(T) was 68.3 mol%. The genome of strain K220(T) has genes associated with 24 biosynthetic gene clusters. The strain contained MK-9(H-4) and iso-C-16: 0 as the predominant respiratory quinone and fatty acid, respectively. The major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine. Based on evidence collected from the genotypic, phenotypic and phylogenetic analyses, strain K220(T) is considered to represent a novel species in the genus Saccharopolyspora, for which the name Saccharopolyspora soli sp. nov. is proposed. The type strain is K220(T) (= JCM 33912(T) = KCTC 49395(T)).
Screening of Acidophilic Actinobacteria That Show Activity against Paddy Pest Fungi
(2021) Veyisoglu, Aysel; Tatar, Demet
This study aimed to isolate and identify acidophilic actinobacteria. Acidophilic actinobacteria isolates were had from a paddy field soil in Osmancık placed near Çorum province in Turkey. The dilution plate technique on seven selective media with pH 5.5 was used for isolation. 16S rRNA gene PCR amplification of acidophilic actinobacteria was performed. Three different algorithms were used in the phylogenetic analyzes made with MEGA 7.0 software. Twenty-two isolates were obtained from seven selective media, and according to 16S rRNA gene sequence analysis of 22 isolates, twenty-one Streptomyces isolates and one Rhodococcus isolate were identified. The antifungal activities of isolated acidophilic actinobacteria against Fusarium moniliforme and Rhizoctonia solani, the rice pathogenic fungi were evaluated. The isolates with antifungal activity have the potential to be used as biological control agents against rice pathogens.
Streptomyces boncukensissp. nov., isolated from saltern soil
(Springer, 2021) Tatar, Demet; Veyisoglu, Aysel; Saygin, Hayrettin; Sahin, Nevzat
A novel Gram-stain positive, aerobic, non-motile Actinobacterium, designated strain SB3404(T), was isolated from saltern soil collected from Boncuk Saltern, Sungurlu-corum, Turkey, and subjected to a polyphasic taxonomic approach. The organism has shown to have phylogenetic, chemotaxonomic, cultural and morphological properties consistent with its classification in the genusStreptomyces. 16S rRNA gene sequence analysis of strain SB3404(T)showed that it is closely related toStreptomyces albusNBRC 13014(T)(97.2% sequence similarity),Streptomyces xishensisYIM M 10378(T)(96.7%) andStreptomyces abyssalisYIM M 10400(T)(96.5%). The cell wall of the strain containedLL-diaminopimelic acid and the cell-wall sugars were glucose, mannose and ribose. The predominant menaquinones were identified as MK-9(H-8) and MK-9(H-6). The major cellular fatty acids were found to beiso-C-16:0,anteiso-C(17:0)andanteiso-C-15:0. Consequently, strain SB3404(T)is considered to represent a novel species in the genusStreptomyces, for which the nameStreptomyces boncukensissp. nov. is proposed. The type strain is SB3404(T)(= KCTC 49371(T) = JCM 34018(T)).
Streptomyces coryli sp. nov., isolated from hazelnut orchard soil
(Microbiology Soc, 2020) Saygin, Hayrettin; Veyisoglu, Aysel; Tatar, Demet; Nigiz, Cengiz; Tokatli, Ali; Sahin, Nevzat
A novel actinobacteria, isolate A7024(T), was isolated from commercial hazelnut orchard soil sample which was collected at Duzce, West Black Sea region, Turkey. A polyphasic taxonomic study was carried out to determine the status of this isolate. The phylogenetic tree reconstructed using the neighbour-joining algorithm based on 165 rRNA gene sequences indicated that isolate A7024(T) was positioned within the members of the genus Streptomyces with the highest sequence similarity (97.7%) to Streptomyces cadmiisoli ZFG47(T). The organism formed an extensively branched substrate and aerial hyphae which generated irregular rod-shaped spores with smooth-surfaces. The cell wall of strain A7024(T) contained LL-diaminopimelic. Glucose, mannose and ribose were detected as whole-cell sugars. Its polar lipids were diphosphatidylglycerol. phosphatidylethanol-amine, phosphatidylinositol, three unidentified phospholipids and three unidentified glycolipids. Major menaquinones were MK-9(H-6) and MK-9(H-4). The major cellular fatty acids were iso-C-16:0, anteiso-C-1(5:0) and anteiso-C-1(7:)0. Strain A7024(T) had a genome size of 9.0 Mb with a genome G+C content of 71.5 mol%. The low level of 16S rRNA gene similarity, 19.3 +/- 2.3% digital DNA-DNA-hybridization and 76.94% average nucleotide identity values, as well as some different phenotypic characteristics allowed the strain to be distinguished from the closely related type strains. Therefore, it is concluded that strain A7024(T) represents a novel species of the genus of Streptomyces, for which the name Streptomyces coryli sp. nov. is proposed. The type strain is A7024(T) (=DSM 42066(T)=KCTC 29102(T)=NRRL B-24888(T)).
Synthesis and characterization of 4-hydroxy-3-((2-hydroxy-3-methoxyben-zylidene)amino)benzene-sulfonic acid and its metal complexes and their antimicrobial activity
(Elsevier, 2025) Yavuz, Senol; Kose, Dursun Ali; Ozkinali, Sevil; Tatar, Demet; Veyisoglu, Aysel
Schiff base was obtained from the reaction of vanillin with 3-amino-4-hydroxybenzenesulfonic acid. Metal complexes were synthesized using (Ni(NO3)2, Co(NO3)2, Mn(NO3)2, Cu(NO3)2, Zn(NO3)2) metal salts and the structures of the compounds were elucidated by spectroscopic methods. The structures of the metal complexes were also characterized by elemental analysis, FT-IR, thermogravimetric analysis and magnetic susceptibility analysis and their antimicrobial activities were investigated. The antimicrobial activities of the obtained coordination compounds were investigated against three Gram-positive (Bacillus subtilis ATCC 6633; Staphylococcus aureus ATCC 25923; Enterococcus faecalis ATCC 29212) three Gram-negative bacterial (Escherichia coli ATCC 25922; Klebsiella pneumonia ATCC 70060; Pseudomonas aeruginosa ATCC 27853) strains and two fungal (Aspergillus niger ATCC 16404; Candida albicans ATCC 1023) strains, and the minimum inhibitory concentration (MIC) values were determined. According to the MIC values, the SC-Ni complex exhibited the highest antimicrobial activity, while the SC-Cu complex showed the lowest.
TİCARİ OLARAK SATILAN Spirulina platensis’İN ÜÇ FARKLI ÇÖZÜCÜ İLE ELDE EDİLEN EKSTRAKTLARININ SEÇİLMİŞ PATOJEN BAKTERİ VE MANTARLARA KARŞI ANTİMİKROBİYAL AKTİVİTELERİNİN BELİRLENMESİ
(2024) Veyisoglu, Aysel; Yeşilyer, Abdulğani Abdulğani; Tatar, Demet
Spirulina cinsi üyelerinin, diğer birçok siyanobakteri türü gibi çok sayıda antimikrobiyal madde üretme potansiyeline sahip olduğu bilinmektedir. Çalışmamızda, ticari olarak satılan toz formundaki Spirulina platensis’in üç farklı çözücü (aseton, hekzan, metanol) kullanılarak elde edilen ekstraktlarının yedi bakteri suşu (Bacillus cereus ATCC 10987, Bacillus subtilis ATCC 6623, Staphylococcus aureus ATCC 25923, Enterococcus faecalis ATCC 29212, Escherichia coli ATCC 25922, Klebsiella pneumoniae ATCC 70060, Pseudomonas aeruginosa ATCC 27853) ve iki fungus türüne (Candida albicans ATCC 10231, Aspergillus niger ATCC 16404) karşı beş farklı konsantrasyonda (30 mg/ml, 15 mg/ml, 6 mg/ml, 3 mg/ml ve 1 mg/ml) disk difüzyon yöntemi kullanılarak antimikrobiyal aktivitesinin belirlenmesi amaçlanmıştır.Sonuçlarımıza göre, kullanılan çözücüler arasında en iyi sonucu metanolden elde edilen ekstraktın verdiği görülmüştür. Metanolden elde edilen ekstrakt Bacillus cereus ATCC 10987, Bacillus subtilis ATCC 6623, Escherichia coli ATCC 25922, Klebsiella pneumoniae ATCC 70060 ve Pseudomonas aeruginosa ATCC 27853 suşlarına karşı antimikrobiyal aktivite gösterirken, hekzandan elde edilen ekstrakt Bacillus cereus ATCC 10987 ve Bacillus subtilis ATCC 6623 suşlarına karşı aktivite göstermiş, asetondan elde edilen ekstrakt ise sadece Pseudomonas aeruginosa ATCC 27853 suşuna karşı antimikrobiyal aktivite göstermiştir. Metanol, aseton ve hekzandan elde edilen ekstraktlar kullanılan funguslara karşı aktivite göstermemiştir.

Yazar "Tatar, Demet" seçeneğine göre listele

Sayfa Başına Sonuç

Sıralama seçenekleri