THE GENETIC HETEROGENEITY OF FACULTATIVE ALKALIPHILIC BACILLUS SPECIES ISOLATED FROM SODA LAKE

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dc.authoridAvsar, cumhur/0000-0002-4095-0022
dc.contributor.authorAvsar, Cumhur
dc.contributor.authorYegin, Zeynep
dc.contributor.authorCivek, Seyhan
dc.contributor.authorBerber, Ismet
dc.date.accessioned2025-03-23T19:47:21Z
dc.date.available2025-03-23T19:47:21Z
dc.date.issued2016
dc.departmentSinop Üniversitesi
dc.description.abstractIn this study, the genotypic diversity of 26 Bacillus strains, including 24 facultative alkaliphilic isolates from the waters and surrounding soils of Lake Van, Turkey and 2 reference strains were investigated. Plasmid profiling and RAPD-PCR were used to determine the genetic variability of the isolates. The plasmid profiling analysis confirmed all the isolates had plasmids and 20 isolates were allocated into three major groups excluding the ones with unique plasmids. A total of six oligonucleotide primers were used for RAPD amplification, which generated a total of 505 fragments, all of them were polymorphic. A numerical analysis of the DNA band profiles with 6 different primers was generated by unweighted pair group method with arithmetic mean (UPGMA). The genetic distances among the isolates using the primers M13-10, M13-14, OPL3, OPL12, RAPD12, and RPO2 were at percentage similarities between 16 to 100%, 12 to 100%, 28 to 94%, 4 to 100%, 31 to 100% and 8 to 80% respectively. The results of the study suggested that RAPD-PCR technique can provide rapid and efficient approach to solve taxonomic difficulties among the facultative alkaliphilic Bacillus isolates at the strain level.
dc.identifier.endpage4110
dc.identifier.issn1018-4619
dc.identifier.issn1610-2304
dc.identifier.issue10
dc.identifier.scopusqualityN/A
dc.identifier.startpage4103
dc.identifier.urihttps://hdl.handle.net/11486/7342
dc.identifier.volume25
dc.identifier.wosWOS:000386746100030
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.language.isoen
dc.publisherParlar Scientific Publications (P S P)
dc.relation.ispartofFresenius Environmental Bulletin
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WOS_20250323
dc.subjectBacillus spp.
dc.subjectRAPD-PCR
dc.subjectLake Van
dc.subjectdendrogram
dc.subjectheterogeneity
dc.titleTHE GENETIC HETEROGENEITY OF FACULTATIVE ALKALIPHILIC BACILLUS SPECIES ISOLATED FROM SODA LAKE
dc.typeArticle

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