The effect of glutathione preconditioning on heme oxygenase-1 system established by whey protein feeding on hepatocellular injury in a rat hepatic normothermic i/r injury model

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dc.contributor.authorManukyan, Manuk N.
dc.contributor.authorYavuz, Yunus
dc.contributor.authorErbarut, Ipek
dc.contributor.authorOgunc, Ayliz Velioglu
dc.contributor.authorAtaizi-Celikel, Cigdem
dc.contributor.authorYalcin, A. Suha
dc.contributor.authorGulluoglu, Bahadir M.
dc.date.accessioned2025-03-23T19:48:10Z
dc.date.available2025-03-23T19:48:10Z
dc.date.issued2008
dc.departmentSinop Üniversitesi
dc.description.abstractPurpose: Oxygen radicals which are produced during reperfusion phase play a central role for hepatocellular damage during ischemia/reperfusion (I/R) injury. Antioxidant strategies appear a promising approach to prevent I/R injury. In the present study, the effect of glutathione (GSH) preconditioning established by whey protein feeding on hepatocellular injury markers was assessed in a rat hepatic normothermic I/R injury model. The interaction between intracellular GSH content and heme oxygenase-1 (HO-1) enzyme expression was also determined in order to understand the level of upgrading of HO-1 system in a high GSH content environment after preconditioning. Material and Methods: Livers of female Sprague-Dawley rats were subjected to 45/45 minutes of normothermic I/R injury. A group of rats were fed by standard chow for a three week duration before I/R procedure (CONTROL I/R; n=16), whereas another group was fed by whey protein for preconditioning (WHPR I/R; n=16) during the same period. Two SHAM groups were constituted accordingly but rats were not subjected to I/R injury (STD SHAM and WHEY SHAM; n=6 in each). Following 45 minutes of reperfusion, serum transaminase levels as well as GSH, malondialdehyde (MDA) levels, apoptotic index (AI) and HO-1 gene expression in liver tissue were determined. Half of the animals in both injury groups were surveilled for a week without sampling. Results: Intracellular GSH levels in WHEY SHAM group were significantly higher than those of STD SHAM group which indicated a successful preconditioning. Preconditioning by whey protein feeding prior to I/R injury (WHPR I/R) significantly ameliorated the increased levels of serum transaminase as well as liver MDA levels and AI in CONTROL I/R group. Survival of the rats in both injury groups were not different. Whey preconditioning also significantly increased GSH levels, whereas HO-1 expression was lower when compared to rats subjected to I/R injury without preconditioning. Conclusion: Feeding with whey protein successfully resulted high GSH content in rat liver. Whey preconditioning ameliorated hepatic I/R injury. HO-1 enzyme seems to have a less important role in antioxidant defense system when GSH production was induced prior to I/R injury.
dc.identifier.endpage144
dc.identifier.issn2564-6850
dc.identifier.issn2564-7032
dc.identifier.issue3
dc.identifier.scopusqualityQ3
dc.identifier.startpage137
dc.identifier.urihttps://hdl.handle.net/11486/7531
dc.identifier.volume24
dc.identifier.wosWOS:000420160600003
dc.identifier.wosqualityN/A
dc.indekslendigikaynakWeb of Science
dc.language.isotr
dc.publisherAves
dc.relation.ispartofTurkish Journal of Surgery
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.snmzKA_WOS_20250323
dc.subjectLiver
dc.subjectischemia/reperfusion
dc.subjectwhey
dc.subjectglutathione
dc.subjectheme oxygenase-1
dc.titleThe effect of glutathione preconditioning on heme oxygenase-1 system established by whey protein feeding on hepatocellular injury in a rat hepatic normothermic i/r injury model
dc.typeArticle

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